Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   MW_RS08230 Genome accession   NC_003923
Coordinates   1670747..1671433 (-) Length   228 a.a.
NCBI ID   WP_001793467.1    Uniprot ID   A0A641AAY3
Organism   Staphylococcus aureus MW2     
Function   dsDNA binding to the cell surface   
DNA binding and uptake

Function

ComEA is anchored to the membrane via an N-terminal membrane-spanning domain. Its C-terminus is located outside the membrane and is required for DNA binding and transport.


Genomic Context

Location: 1665747..1676433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW_RS08210 (MW1537) rpsT 1666661..1666912 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  MW_RS08215 (MW1538) holA 1666957..1667931 (-) 975 WP_001282568.1 DNA polymerase III subunit delta -
  MW_RS08220 (MW1539) comEC 1667988..1670189 (-) 2202 WP_001566670.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MW_RS08225 (MW1540) - 1670194..1670655 (-) 462 WP_000439693.1 ComE operon protein 2 -
  MW_RS08230 (MW1541) comEA 1670747..1671433 (-) 687 WP_001793467.1 ComEA family DNA-binding protein Machinery gene
  MW_RS08235 (MW1542) - 1671473..1672189 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  MW_RS08240 (MW1543) rsfS 1672192..1672545 (-) 354 WP_001088022.1 ribosome silencing factor -
  MW_RS08245 (MW1544) yqeK 1672546..1673130 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MW_RS08250 (MW1545) nadD 1673120..1673689 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  MW_RS08255 (MW1546) yhbY 1673692..1673982 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  MW_RS08260 (MW1547) aroE 1673986..1674792 (-) 807 WP_000666748.1 shikimate dehydrogenase -
  MW_RS08265 (MW1548) yqeH 1674806..1675906 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH comEA positive effect
  sigH comGD positive effect
  comK/comK1 comGD positive effect
  sigH dprA positive effect
  comK/comK1 dprA positive effect
  sigH comGA positive effect
  comK/comK1 comGA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  sigH comGF positive effect
  comK/comK1 comGF positive effect
  sigH comEC positive effect
  comK/comK1 comEC positive effect
  sigH comGB positive effect
  comK/comK1 comGB positive effect
  comK/comK1 comEA positive effect
  sigH comGC positive effect
  comK/comK1 comGC positive effect
  sigH coiA positive effect
  comK/comK1 coiA positive effect
  sigH comGE positive effect
  comK/comK1 comGE positive effect

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25786.50 Da        Isoelectric Point: 9.6753

>NTDB_id=36 MW_RS08230 WP_001793467.1 1670747..1671433(-) (comEA) [Staphylococcus aureus MW2]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=36 MW_RS08230 WP_001793467.1 1670747..1671433(-) (comEA) [Staphylococcus aureus MW2]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATAATG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A641AAY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

97.368

100

0.974
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

57.377

77.215

0.443
  comEA Bacillus subtilis subsp. subtilis str. 168

37.273

100

0.4
  comEA Lactococcus lactis subsp. cremoris KW2

37.037

99.083

0.367
  comEA Legionella pneumophila strain ERS1305867

38.384

94.286

0.362
  comEA Legionella pneumophila str. Paris

38.384

94.286

0.362

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]