Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   MW_RS08220 Genome accession   NC_003923
Coordinates   1667988..1670189 (-) Length   733 a.a.
NCBI ID   WP_001566670.1    Uniprot ID   -
Organism   Staphylococcus aureus MW2     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComEC is essential for transformation, and is proposed to form a channel for passage of exogenous DNA into recipient cells


Genomic Context

Location: 1662988..1675189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW_RS08205 (MW1536) lepA 1664492..1666315 (-) 1824 WP_000368337.1 translation elongation factor 4 -
  MW_RS08210 (MW1537) rpsT 1666661..1666912 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  MW_RS08215 (MW1538) holA 1666957..1667931 (-) 975 WP_001282568.1 DNA polymerase III subunit delta -
  MW_RS08220 (MW1539) comEC 1667988..1670189 (-) 2202 WP_001566670.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MW_RS08225 (MW1540) - 1670194..1670655 (-) 462 WP_000439693.1 ComE operon protein 2 -
  MW_RS08230 (MW1541) comEA 1670747..1671433 (-) 687 WP_001793467.1 ComEA family DNA-binding protein Machinery gene
  MW_RS08235 (MW1542) - 1671473..1672189 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  MW_RS08240 (MW1543) rsfS 1672192..1672545 (-) 354 WP_001088022.1 ribosome silencing factor -
  MW_RS08245 (MW1544) yqeK 1672546..1673130 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MW_RS08250 (MW1545) nadD 1673120..1673689 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  MW_RS08255 (MW1546) yhbY 1673692..1673982 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  MW_RS08260 (MW1547) aroE 1673986..1674792 (-) 807 WP_000666748.1 shikimate dehydrogenase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH comEC positive effect
  sigH comGD positive effect
  comK/comK1 comGD positive effect
  sigH dprA positive effect
  comK/comK1 dprA positive effect
  sigH comGA positive effect
  comK/comK1 comGA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  sigH comGF positive effect
  comK/comK1 comGF positive effect
  comK/comK1 comEC positive effect
  sigH comGB positive effect
  comK/comK1 comGB positive effect
  sigH comEA positive effect
  comK/comK1 comEA positive effect
  sigH comGC positive effect
  comK/comK1 comGC positive effect
  sigH coiA positive effect
  comK/comK1 coiA positive effect
  sigH comGE positive effect
  comK/comK1 comGE positive effect

Sequence

Protein


Download         Length: 733 a.a.        Molecular weight: 84904.95 Da        Isoelectric Point: 10.0384

>NTDB_id=38 MW_RS08220 WP_001566670.1 1667988..1670189(-) (comEC) [Staphylococcus aureus MW2]
MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQ
VIQIQRQGSDTYKGRLSLKNEIYPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYKPLKRLNLPLFVIKGIT
IIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQ
QLSKLQSLFIITFIAQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFH
DWLLDLFTRIKQSHFSVTKFNDWIFIIFIISVYYIFWLLAKRKYILVTVWTIIILTLLITFPTNSHHKITMLNVGQGDSI
LYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIEIYNKG
YSSNTLMLLSKLSHKYNIKLIDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLL
KKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNL
KVDSNSYGNASGL

Nucleotide


Download         Length: 2202 bp        

>NTDB_id=38 MW_RS08220 WP_001566670.1 1667988..1670189(-) (comEC) [Staphylococcus aureus MW2]
TTGCTGTATGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTCTCTACATTTCTTTTCATTTT
ACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTATGCCCCTATTTCTCTCTTTTTAATCATTTTCTCCTCATGGT
ATTTACATTATTCACAACAAGCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAATGAAATATATCCTTTCTTTTT
AACAAATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGTCGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGACA
ATAAGTTTGTAACTCTTAAATTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
CATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATTATGGCATTGATTACTGGTGA
CGTAAAAGAAATTAATGAGCAATTTAAGGAACGTGTTAAAGAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGC
ATATAGCTGCAATTGTATTCTTAATTTACAAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
ATCATTGTATTAGCTTTATTTGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCTATAATAATGACAACTCTTGT
ACTGCTTATTACTAAGCAAATTAAAATAAAGGGTATTCAGCTATTAGCATTTGCATTTATAATTATGTTTATTTTAAATC
CACTAGTTGTTTATGATATTGGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTTATCGTTGCCATTCCAAACTT
TCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATTTTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTA
TTTTATTCTTTATTACAAGTCATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
GACTGGTTACTAGACCTATTCACAAGAATCAAGCAATCACATTTTTCTGTTACCAAGTTTAATGATTGGATATTTATAAT
ATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCTAAACGTAAATATATATTGGTTACGGTTTGGACTATAATTA
TTCTGACATTATTAATAACGTTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
TTATATGAAGGTGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGATGATACTAAACAACCTAGTTA
TTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAAAGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATC
CACACAATGACCATATTGGTGAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGAGATATACAATAAGGGA
TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTTATAGATGTAAGACAAGTTAG
TAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGATAGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGA
TTATTACTATGATTACATATCAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGTCATCATGGGAGCAAGACAAGTAGTTCTAAAGAATTTAT
AGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGGAAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTA
AACGATTGCAAAGGATTCGCAGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
AAAGTTGATTCAAACTCTTATGGAAATGCAAGTGGTTTATAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(183-441)

Metallo-beta-lactamase superfamily (Lactamase_B)

(474-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Staphylococcus aureus N315

98.772

100

0.988

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]