Detailed information    

experimental Experimentally validated

Overview

Name   comK/comK1   Type   Regulator
Locus tag   MW_RS04875 Genome accession   NC_003923
Coordinates   1002905..1003474 (-) Length   189 a.a.
NCBI ID   WP_000287265.1    Uniprot ID   A0A7U7EXU5
Organism   Staphylococcus aureus MW2     
Function   promote expression of competence genes   
Competence regulation

Function

ComK1 plays a role in regulating transcription of competence genes in S. aureus.


Genomic Context

Location: 997905..1008474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW_RS04855 (MW0904) - 999410..1000768 (+) 1359 WP_000021864.1 TrkH family potassium uptake protein -
  MW_RS04860 (MW0905) - 1000907..1002418 (+) 1512 WP_000414174.1 bifunctional metallophosphatase/5'-nucleotidase -
  MW_RS04875 (MW0906) comK/comK1 1002905..1003474 (-) 570 WP_000287265.1 competence protein ComK Regulator
  MW_RS04880 (MW0907) - 1003684..1003902 (+) 219 WP_000876826.1 IDEAL domain-containing protein -
  MW_RS04885 (MW0908) - 1003983..1004969 (-) 987 WP_000668820.1 lipoate--protein ligase -
  MW_RS04890 (MW0909) - 1005168..1005344 (+) 177 WP_000214898.1 YkvS family protein -
  MW_RS04895 (MW0910) - 1005359..1005961 (+) 603 WP_001033867.1 type II CAAX prenyl endopeptidase Rce1 family protein -
  MW_RS15305 - 1006217..1006279 (+) 63 WP_228764722.1 hypothetical protein -
  MW_RS04905 - 1006818..1006919 (+) 102 WP_031893173.1 hypothetical protein -
  MW_RS14610 - 1006929..1007201 (+) 273 WP_001797239.1 lactococcin 972 family bacteriocin -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comK/comK1 comGA positive effect
  sigH comGA positive effect
  sigH comGD positive effect
  sigH dprA positive effect
  sigH ssb positive effect
  sigH comGF positive effect
  sigH comEC positive effect
  sigH comGB positive effect
  sigH comEA positive effect
  sigH comGC positive effect
  sigH coiA positive effect
  sigH comGE positive effect
  comK/comK1 comGD positive effect
  comK/comK1 ssb positive effect
  comK/comK1 dprA positive effect
  comK/comK1 comGE positive effect
  comK/comK1 comGB positive effect
  comK/comK1 comEA positive effect
  comK/comK1 comGC positive effect
  comK/comK1 coiA positive effect
  comK/comK1 comGF positive effect
  comK/comK1 comEC positive effect

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 22601.83 Da        Isoelectric Point: 9.6091

>NTDB_id=39 MW_RS04875 WP_000287265.1 1002905..1003474(-) (comK/comK1) [Staphylococcus aureus MW2]
MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKVQKIIERSCKFYGNTYLGKKAETNRITGISSKPPIL
LTPLFPTYFFPTHSDRQNENIWLNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
KNPDQPIDYNKATLNVFEALTRYSLFEDK

Nucleotide


Download         Length: 570 bp        

>NTDB_id=39 MW_RS04875 WP_000287265.1 1002905..1003474(-) (comK/comK1) [Staphylococcus aureus MW2]
ATGTATTCTCAAAATATTTATGTGATACGCAAAGGAGACATGGTTATTCGACCAGCATTTGATGATGACGATCAAAGAAA
CGGTAGTGAAATAATTCGGTTTGACAAAACGCGTATTCAAAATCCTTTTAAAGTCCAGAAAATCATTGAACGCTCTTGCA
AATTTTATGGTAATACTTATCTTGGCAAGAAAGCAGAGACAAACCGCATTACTGGCATTTCTAGTAAACCACCTATTTTA
CTAACACCATTATTTCCAACTTATTTTTTCCCAACACATTCTGACAGACAAAATGAAAATATTTGGTTAAATATGCATTA
TATCGAAAGTATTAAAGAATTAAAAAATCGTAAATGTAAAGTGACATTTATTAATAATGAATCAATCATTCTTCATGTTT
CATACCACAGTTTATGGCATCAATATAACAATTCCATTTTTTACTATTACATGGTAGATAAACAATCTCGCATGATATCA
AAAAATCCCGACCAACCAATAGATTATAATAAAGCCACATTGAATGTGTTTGAAGCATTGACACGCTATTCTTTATTTGA
AGATAAATAA

Domains

Predicted by InterproScan.

ComK protein (ComK)

(5-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EXU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comK/comK1 Staphylococcus aureus N315

100

100

1

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]