Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   MW_RS04740 Genome accession   NC_003923
Coordinates   972410..973396 (+) Length   328 a.a.
NCBI ID   WP_000959293.1    Uniprot ID   A0A641A950
Organism   Staphylococcus aureus MW2     
Function   homologous recombination   
Homologous recombination

Function

The proteins encoded by dprA and coiA are both known to be required for competence development in transformable bacterial species.


Genomic Context

Location: 967410..978396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW_RS04715 (MW0876) opp4B 967623..968585 (+) 963 WP_000239820.1 oligopeptide ABC transporter permease -
  MW_RS04720 (MW0877) opp4C 968597..969478 (+) 882 WP_001180274.1 oligopeptide ABC transporter permease -
  MW_RS04725 (MW0878) trpS 969520..970509 (-) 990 WP_000448934.1 tryptophan--tRNA ligase -
  MW_RS04730 (MW0879) spxA 970804..971199 (+) 396 WP_000258003.1 transcriptional regulator SpxA -
  MW_RS04735 (MW0880) mecA 971570..972289 (+) 720 WP_001217728.1 adaptor protein MecA -
  MW_RS04740 (MW0881) coiA 972410..973396 (+) 987 WP_000959293.1 competence protein CoiA Machinery gene
  MW_RS04745 (MW0882) pepF 973444..975252 (+) 1809 WP_000082725.1 oligoendopeptidase F -
  MW_RS04750 (MW0883) yjbH 975712..976518 (-) 807 WP_000896697.1 protease adaptor protein YjbH -
  MW_RS04755 (MW0884) - 976541..976906 (-) 366 WP_000214070.1 truncated hemoglobin YjbI -
  MW_RS04760 (MW0885) - 977010..977603 (-) 594 WP_000224612.1 CYTH domain-containing protein -
  MW_RS04765 (MW0886) - 977789..978136 (+) 348 WP_001242102.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH coiA positive effect
  sigH comGD positive effect
  comK/comK1 comGD positive effect
  sigH dprA positive effect
  comK/comK1 dprA positive effect
  sigH comGA positive effect
  comK/comK1 comGA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  sigH comGF positive effect
  comK/comK1 comGF positive effect
  sigH comEC positive effect
  comK/comK1 comEC positive effect
  sigH comGB positive effect
  comK/comK1 comGB positive effect
  sigH comEA positive effect
  comK/comK1 comEA positive effect
  sigH comGC positive effect
  comK/comK1 comGC positive effect
  comK/comK1 coiA positive effect
  sigH comGE positive effect
  comK/comK1 comGE positive effect

Sequence

Protein


Download         Length: 328 a.a.        Molecular weight: 38898.54 Da        Isoelectric Point: 9.7732

>NTDB_id=42 MW_RS04740 WP_000959293.1 972410..973396(+) (coiA) [Staphylococcus aureus MW2]
MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHFAHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGFKV
EIEPFLKEIKQIPDILINNKYVIELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKL
HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQYIKYCRWQNSVLEPTLSAMYQLQ
LTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLKNGKSKLVNDNLNYFKLKKFIVGLESKTAIIEKLINNYLNICSDR
GNDVQILF

Nucleotide


Download         Length: 987 bp        

>NTDB_id=42 MW_RS04740 WP_000959293.1 972410..973396(+) (coiA) [Staphylococcus aureus MW2]
ATGTTAGTAGCTTTAAATGAAGAAAAGGAACGTGTGTTAGCAACTACTGCATTGAGAAAGACACAATATTTTTGTCCGGT
GTGTGGCAAGCAAGTTATTTTAAAGCGTGGGCTCAAAGTAATTAGTCATTTTGCACATAAACATTTAGCGGAACAAAAAT
GTTTTAATAATGAAACGATTAAACATTATAAAAGTAAATTGATTTTAGCACAGATGATACAGCAACAAGGATTTAAAGTA
GAGATAGAGCCATTTTTAAAAGAAATAAAACAAATTCCGGATATTTTGATTAATAATAAATATGTTATTGAGCTACAGTA
TTCGCCAATTCCTTATAAACAGATTCTTCAACGAACGGAAGGTTTAAAGAAAATGGGATATAAAGTAAGTTGGTTATTAA
ATGATGTTGATTATTGTCATAATAAAGTGAAGTTCAATCATTTTCAAAGTATGTTTATTAATCCATTCACTCGAAAACTT
CATACGTTCAATTTAGAGAAAAAACAAATAATGATGTTTCAACAAATACAATATTTAGGCGGGCACAAATATGTCGCTGA
AAAAAGAAATGCCAAAATTAGTGAGTTGTTTAATGAGGCGCCTTGTGATTATCATGCTGTTTATAAATTATCAAAGTTCG
CAATTAATCAATATATCAAATATTGTCGCTGGCAAAATTCTGTTTTAGAACCCACTTTAAGTGCAATGTATCAATTACAG
TTAACTGATCAAGAAGTAGTGCACAATTATGGTTATATTTTTCCAGAGCAAATTTATATTAAAAATCATCCTATTGAGTG
GCAATTACAAGTTGATTTATGGTTAAAGAATGGAAAAAGCAAATTAGTAAATGACAATCTTAATTATTTTAAACTGAAAA
AGTTTATTGTTGGTCTAGAAAGTAAAACAGCAATTATAGAAAAACTTATTAACAATTATTTAAATATTTGTTCAGATAGA
GGTAATGACGTGCAAATTTTGTTCTAA

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(6-306)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A641A950

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  coiA Staphylococcus aureus N315

97.561

100

0.976

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]