Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   MW_RS06080 Genome accession   NC_003923
Coordinates   1236282..1237154 (+) Length   290 a.a.
NCBI ID   WP_000593192.1    Uniprot ID   A0A140HKN1
Organism   Staphylococcus aureus MW2     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

Similar to SsbB, DprA and RecA bind internalized ssDNA and protect it from degradation. DprA displaces SsbB and stimulates loading of RecA onto the ssDNA fragment.


Genomic Context

Location: 1231282..1242154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW_RS06060 (MW1128) sucC 1231396..1232562 (+) 1167 WP_001020801.1 ADP-forming succinate--CoA ligase subunit beta -
  MW_RS06065 (MW1129) sucD 1232584..1233492 (+) 909 WP_000110251.1 succinate--CoA ligase subunit alpha -
  MW_RS06070 (MW1130) - 1233719..1234837 (+) 1119 WP_001041666.1 LysM peptidoglycan-binding domain-containing protein -
  MW_RS06075 (MW1131) fmhC 1234865..1236109 (+) 1245 WP_000672867.1 FemA/FemB family glycyltransferase FmhC -
  MW_RS06080 (MW1132) dprA 1236282..1237154 (+) 873 WP_000593192.1 DNA-processing protein DprA Machinery gene
  MW_RS06085 (MW1133) topA 1237328..1239403 (+) 2076 WP_001557331.1 type I DNA topoisomerase -
  MW_RS06090 (MW1134) trmFO 1239559..1240866 (+) 1308 WP_000195254.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH dprA positive effect
  sigH comGD positive effect
  comK/comK1 comGD positive effect
  comK/comK1 dprA positive effect
  sigH comGA positive effect
  comK/comK1 comGA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  sigH comGF positive effect
  comK/comK1 comGF positive effect
  sigH comEC positive effect
  comK/comK1 comEC positive effect
  sigH comGB positive effect
  comK/comK1 comGB positive effect
  sigH comEA positive effect
  comK/comK1 comEA positive effect
  sigH comGC positive effect
  comK/comK1 comGC positive effect
  sigH coiA positive effect
  comK/comK1 coiA positive effect
  sigH comGE positive effect
  comK/comK1 comGE positive effect

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 33564.99 Da        Isoelectric Point: 9.5094

>NTDB_id=40 MW_RS06080 WP_000593192.1 1236282..1237154(+) (dprA) [Staphylococcus aureus MW2]
MIKLFLLKLYWAHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEWVNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYI
TYMDSEYPVLLKEIYQFPLLLFYKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNRIISGLSKGVLITEAKEQSGSHI
TIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAKVVLNANDIFEDYYI

Nucleotide


Download         Length: 873 bp        

>NTDB_id=40 MW_RS06080 WP_000593192.1 1236282..1237154(+) (dprA) [Staphylococcus aureus MW2]
TTGATTAAACTATTTTTGCTTAAGTTATACTGGGCACACTTTTCGACTAAACAAATTCATCAATTTTTAATGGCATATCC
TAATGTAATTAAAGAGGAGGGAAGAAAAAAAGATAGTTATTTATGTGAATGGGTGAATAGGGAAGAAAATGTTCATTTAT
TACGTAAATACTATGCTTTTATAAAACTTGATCATAACGATATTATTAAAGAACTGCAGAAATTAAAAGTAAGTTACATT
ACATATATGGATTCTGAATACCCAGTGCTATTAAAAGAAATATATCAATTTCCATTACTTCTTTTCTATAAAGGGAACAT
CAAATTAATAAATAATATGCATCATTTGGCAGTAGTAGGTGCAAGAGATTCTACAAGTTATACCCAACAGTCTTTAGAAT
TTTTATTATCAAATGATAAAAGCAAATATTTAACAATTGTTTCCGGCCTTGCTCAAGGAGCTGATGCAATGGCACATCAA
ATAGCTTTAAAATACAATCTCCCTACAATTGCAGTTTTAGCCTTTGGCCATCAAACACATTATCCCAAAAGTACATTAGC
ATTAAGAAATAAAATAGAAGAAAAAGGTTTAGTTATATCCGAATATCCACCACATACACCAATTGCTAAATATAGATTTC
CTGAGCGCAATAGAATTATCAGCGGTTTGTCAAAAGGGGTTTTAATTACTGAGGCTAAGGAACAAAGTGGCAGTCACATC
ACGATAGATTTTGCATTAGAGCAAAATAGAAATGTTTATGTTTTACCTGGATCTATGTTTAATCCTATGACAAAAGGTAA
TTTATTACGTATCCAAGAAGGTGCTAAGGTAGTATTAAACGCTAATGATATATTTGAAGACTACTATATTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(72-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A140HKN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Staphylococcus aureus N315

99.655

100

0.997

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]