Detailed information    

experimental Experimentally validated

Overview

Name   sigH   Type   Regulator
Locus tag   MW_RS02705 Genome accession   NC_003923
Coordinates   564872..565441 (+) Length   189 a.a.
NCBI ID   WP_000872877.1    Uniprot ID   A0A641A2F3
Organism   Staphylococcus aureus MW2     
Function   promote expression of competence genes   
Competence regulation

Function

An over-expression of this sigma factor in S. aureus resulted in a drastic induction of the expression of the com operons that encode proteins required for the natural genetic competence.


Genomic Context

Location: 559872..570441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW_RS02680 (MW0484) cysE 561085..561732 (+) 648 WP_001805237.1 serine O-acetyltransferase -
  MW_RS02685 (MW0485) cysS 561716..563116 (+) 1401 WP_000631973.1 cysteine--tRNA ligase -
  MW_RS02690 (MW0486) - 563109..563513 (+) 405 WP_000370182.1 Mini-ribonuclease 3 -
  MW_RS02695 (MW0487) rlmB 563521..564267 (+) 747 WP_000390332.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MW_RS02700 (MW0488) - 564267..564791 (+) 525 WP_000664738.1 NYN domain-containing protein -
  MW_RS02705 (MW0489) sigH 564872..565441 (+) 570 WP_000872877.1 sigma-70 family RNA polymerase sigma factor Regulator
  MW_RS14500 rpmG 565556..565699 (+) 144 WP_001788193.1 50S ribosomal protein L33 -
  MW_RS02710 (MW0490) secE 565755..565937 (+) 183 WP_001074473.1 preprotein translocase subunit SecE -
  MW_RS02715 (MW0491) nusG 565950..566498 (+) 549 WP_001288302.1 transcription termination/antitermination protein NusG -
  MW_RS02720 (MW0492) rplK 566679..567101 (+) 423 WP_001085792.1 50S ribosomal protein L11 -
  MW_RS02725 (MW0493) rplA 567309..568001 (+) 693 WP_001074619.1 50S ribosomal protein L1 -
  MW_RS02730 (MW0494) rplJ 568273..568773 (+) 501 WP_001273085.1 50S ribosomal protein L10 -
  MW_RS02735 (MW0495) rplL 568816..569184 (+) 369 WP_001273586.1 50S ribosomal protein L7/L12 -
  MW_RS02740 (MW0496) - 569359..569967 (+) 609 WP_000020302.1 class I SAM-dependent methyltransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH comGD positive effect
  sigH dprA positive effect
  sigH comGA positive effect
  sigH ssb positive effect
  sigH comGF positive effect
  sigH comEC positive effect
  sigH comGB positive effect
  sigH comEA positive effect
  sigH comGC positive effect
  sigH coiA positive effect
  sigH comGE positive effect
  comK/comK1 comGD positive effect
  comK/comK1 comGA positive effect
  comK/comK1 ssb positive effect
  comK/comK1 dprA positive effect
  comK/comK1 comGE positive effect
  comK/comK1 comGB positive effect
  comK/comK1 comEA positive effect
  comK/comK1 comGC positive effect
  comK/comK1 coiA positive effect
  comK/comK1 comGF positive effect
  comK/comK1 comEC positive effect

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 23126.01 Da        Isoelectric Point: 9.6015

>NTDB_id=29 MW_RS02705 WP_000872877.1 564872..565441(+) (sigH) [Staphylococcus aureus MW2]
MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFNHYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQC
LITSVKYDYLRKYLATNKRMDNLINEYRVTYPCAIKRYDVENNYLNKLAIKELIRQFKYLSVFEKDVMYLMCEQYKPREI
AQLMHVKEKVIYNAIQRCKNKIKRYFKMI

Nucleotide


Download         Length: 570 bp        

>NTDB_id=29 MW_RS02705 WP_000872877.1 564872..565441(+) (sigH) [Staphylococcus aureus MW2]
TTGAAATACGATTTGACAACTCAAGACAGTACAATCAAACGTAACAATGCAATAAATGATAAAGACTTCGAAAAGTTAGT
AATGGATCTACAACCATTAATTATTCGACGCATCAAAACATTTGGATTTAATCATTATGATTTAGAAGACTTATATCAAG
AAATACTTATACGGATGTATAGGTCGGTCCAAACATTTGATTTTAGTGGAGAGCAGCCTTTCACAAATTATGTTCAATGT
TTAATTACGTCTGTAAAGTATGATTATTTGAGAAAATATTTAGCTACAAATAAAAGAATGGATAATTTGATTAATGAATA
TAGAGTTACGTATCCATGTGCAATAAAGCGTTATGATGTTGAAAACAATTATTTGAATAAATTAGCAATTAAAGAGTTGA
TTCGTCAGTTTAAGTATTTGAGTGTATTTGAAAAAGATGTCATGTATTTAATGTGTGAACAATATAAGCCGAGAGAAATT
GCTCAACTGATGCATGTAAAAGAGAAAGTGATTTATAATGCCATACAACGATGTAAAAATAAAATAAAACGTTATTTCAA
AATGATTTGA

Domains

Predicted by InterproScan.

Sigma-70 region 2 (Sigma70_r2)

(26-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A641A2F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sigH Staphylococcus aureus N315

97.884

100

0.979

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]