Detailed information    

experimental Experimentally validated

Overview

Name   coiA   Type   Machinery gene
Locus tag   SA_RS04860 Genome accession   NC_002745
Coordinates   972441..973427 (+) Length   328 a.a.
NCBI ID   WP_000959282.1    Uniprot ID   A0A7U7EXT8
Organism   Staphylococcus aureus N315     
Function   homologous recombination   
Homologous recombination

Function

The proteins encoded by dprA and coiA are both known to be required for competence development in transformable bacterial species.


Genomic Context

Location: 967441..978427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA_RS04835 (SA0853) opp4B 967655..968617 (+) 963 WP_000915997.1 oligopeptide ABC transporter permease -
  SA_RS04840 (SA0854) opp4C 968629..969510 (+) 882 WP_001180280.1 oligopeptide ABC transporter permease -
  SA_RS04845 (SA0855) trpS 969552..970541 (-) 990 WP_000448934.1 tryptophan--tRNA ligase -
  SA_RS04850 (SA0856) spxA 970835..971230 (+) 396 WP_000258003.1 transcriptional regulator SpxA -
  SA_RS04855 (SA0857) mecA 971601..972320 (+) 720 WP_001217728.1 adaptor protein MecA -
  SA_RS04860 (SA0858) coiA 972441..973427 (+) 987 WP_000959282.1 competence protein CoiA Machinery gene
  SA_RS04865 (SA0859) pepF 973475..975283 (+) 1809 WP_000082725.1 oligoendopeptidase F -
  SA_RS04870 (SA0860) yjbH 975743..976549 (-) 807 WP_000896697.1 protease adaptor protein YjbH -
  SA_RS04875 (SA0861) - 976572..976937 (-) 366 WP_000214070.1 truncated hemoglobin YjbI -
  SA_RS04880 (SA0862) - 977041..977634 (-) 594 WP_000224619.1 CYTH domain-containing protein -
  SA_RS04885 (SA0863) - 977820..978167 (+) 348 WP_001242103.1 hypothetical protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sigH coiA positive effect
  sigH comGD positive effect
  agrC comGD positive effect
  braS comGD positive effect
  braR comGD positive effect
  vraR comGD negative effect
  agrA comGD positive effect
  comK/comK1 comGD positive effect
  vraS comGD negative effect
  agrC comGB positive effect
  braR comGB positive effect
  sigH comGB positive effect
  braS comGB positive effect
  vraS comGB negative effect
  agrA comGB positive effect
  comK/comK1 comGB positive effect
  vraR comGB negative effect
  agrC comGC positive effect
  vraS comGC negative effect
  agrA comGC positive effect
  comK/comK1 comGC positive effect
  vraR comGC negative effect
  braR comGC positive effect
  sigH comGC positive effect
  braS comGC positive effect
  agrC comGE positive effect
  vraS comGE negative effect
  agrA comGE positive effect
  comK/comK1 comGE positive effect
  vraR comGE negative effect
  braR comGE positive effect
  sigH comGE positive effect
  braS comGE positive effect
  agrC comGA positive effect
  agrA comGA positive effect
  comK/comK1 comGA positive effect
  vraR comGA negative effect
  vraS comGA negative effect
  sigH comGA positive effect
  braS comGA positive effect
  braR comGA positive effect
  agrC comGF positive effect
  agrA comGF positive effect
  comK/comK1 comGF positive effect
  vraR comGF negative effect
  vraS comGF negative effect
  sigH comGF positive effect
  braS comGF positive effect
  braR comGF positive effect
  comK/comK1 coiA positive effect
  sigH positive effect
  comK/comK1 positive effect
  sigH comEA positive effect
  comK/comK1 comEA positive effect
  sigH ssb positive effect
  comK/comK1 ssb positive effect
  sigH dprA positive effect
  comK/comK1 dprA positive effect

Sequence

Protein


Download         Length: 328 a.a.        Molecular weight: 38878.55 Da        Isoelectric Point: 9.5714

>NTDB_id=26 SA_RS04860 WP_000959282.1 972441..973427(+) (coiA) [Staphylococcus aureus N315]
MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHFAHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKV
EIEPFLKEIKQIPDILINNKYVIELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFHSLFINPITRKL
HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKIIELFNEAPCDYHAVYKLSKFAINQYIKYCRWQNSVLEPTLSAMYQLQ
LTDQEVVYNYGYIFPEQIYIENHPIEWQLQVDLWLKNGKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNICSDR
GNDVQILF

Nucleotide


Download         Length: 987 bp        

>NTDB_id=26 SA_RS04860 WP_000959282.1 972441..973427(+) (coiA) [Staphylococcus aureus N315]
ATGTTAGTAGCTTTAAATGAAGAAAAGGAACGTGTGTTAGCAACTACTGCATTGAGAAAGACACAATATTTTTGTCCTGT
GTGTGGCAAGCAAGTTATTTTAAAGCGTGGGCTCAAAGTAATTAGTCATTTTGCACATAAACATTTAGCGGAACAAAAAT
GTTTTAATAATGAAACGATTAAACATTATAAAAGTAAATTGATTTTAGCACAGATGATACAGCAACAAGGATGTAAAGTA
GAGATAGAGCCATTTTTAAAAGAAATAAAACAAATTCCGGATATTTTGATTAATAATAAATATGTTATTGAGCTACAGTA
TTCGCCAATTCCTTATAAACAGATTCTTCAACGAACGGAAGGTTTAAAGAAAATGGGATATAAAGTAAGTTGGTTATTAA
ATGATGTTGATTATTGTCATAATAAAGTGAAGTTCAATCATTTTCATAGTTTGTTTATTAATCCAATCACTCGAAAACTT
CATACGTTCAATTTAGAGAAAAAACAAATAATGATGTTTCAACAAATACAATATTTAGGCGGGCACAAATATGTCGCTGA
AAAAAGGAATGCCAAAATTATTGAGTTGTTTAATGAGGCGCCTTGTGATTATCATGCTGTTTATAAATTATCAAAGTTCG
CAATTAATCAATATATCAAATATTGTCGCTGGCAAAATTCTGTTTTAGAACCCACTTTAAGTGCAATGTATCAATTACAG
TTAACTGATCAAGAAGTAGTGTACAATTATGGTTATATTTTTCCAGAGCAAATTTATATTGAAAATCATCCAATTGAGTG
GCAATTACAAGTTGATTTATGGTTAAAGAATGGAAAAAGCAAATTAGTAAATGACAATCTTAATTATTTTAAACTGAAAA
AATTTATTGTTGCTCTAGAAAGTAAAACAGCAATTATAGAAAAACTTATTAACAATTATTTAAATATTTGTTCAGATAGA
GGTAATGACGTGCAAATTTTGTTCTAA

Domains

Predicted by InterproScan.

Competence protein CoiA-like family (CoiA_nuc)

(6-308)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EXT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  coiA Staphylococcus aureus MW2

97.561

100

0.976

Multiple sequence alignment    



References

[1] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]