Detailed information    

experimental Experimentally validated

Overview

Name   comEC   Type   Machinery gene
Locus tag   SA_RS07995 Genome accession   NC_002745
Coordinates   1622337..1624538 (-) Length   733 a.a.
NCBI ID   WP_001557362.1    Uniprot ID   A0A6K1MRM3
Organism   Staphylococcus aureus N315     
Function   ssDNA transport into the cell   
DNA binding and uptake

Function

ComEC is essential for transformation, and is proposed to form a channel for passage of exogenous DNA into recipient cells


Genomic Context

Location: 1617337..1629538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA_RS07980 (SA1413) lepA 1618841..1620664 (-) 1824 WP_000368338.1 translation elongation factor 4 -
  SA_RS07985 (SA1414) rpsT 1621010..1621261 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  SA_RS07990 (SA1415) holA 1621306..1622280 (-) 975 WP_001282560.1 DNA polymerase III subunit delta -
  SA_RS07995 (SA1416) comEC 1622337..1624538 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SA_RS08000 (SA1417) - 1624543..1625004 (-) 462 WP_000439693.1 ComE operon protein 2 -
  SA_RS08005 (SA1418) comEA 1625096..1625782 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  SA_RS08010 (SA1419) - 1625822..1626538 (-) 717 WP_000084830.1 class I SAM-dependent DNA methyltransferase -
  SA_RS08015 (SA1420) rsfS 1626541..1626894 (-) 354 WP_001088020.1 ribosome silencing factor -
  SA_RS08020 (SA1421) yqeK 1626895..1627479 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SA_RS08025 (SA1422) nadD 1627469..1628038 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  SA_RS08030 (SA1423) yhbY 1628041..1628331 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  SA_RS08035 (SA1424) aroE 1628335..1629141 (-) 807 WP_000666757.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 733 a.a.        Molecular weight: 84998.05 Da        Isoelectric Point: 10.0028

>NTDB_id=22 SA_RS07995 WP_001557362.1 1622337..1624538(-) (comEC) [Staphylococcus aureus N315]
MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQ
VIQIQRQGSDTYKGRLSLKNEIYPFFLTDKKNFDLKKIESRNCIVKGQFKVNENKFVTLKLQSIVVQSCLESNRSNLIEK
HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYQPLKRLNLPLFVIKGIT
IIVLTLYAQYTNYAPSAVRAIIMTTLVLLITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQ
QLSKLQSLFIITFIAQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFH
DWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSI
LYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKG
YSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLL
KKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNL
KVDSNSYGNASGL

Nucleotide


Download         Length: 2202 bp        

>NTDB_id=22 SA_RS07995 WP_001557362.1 1622337..1624538(-) (comEC) [Staphylococcus aureus N315]
TTGCTGTATGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTCTCTACATTTCTTTTCATTTT
ACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTATGCCCCTATTTCTCTCTTTTTAATCATTTTCTCCTCATGGT
ATTTACATTATTCACAACAAGCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAATGAAATATATCCTTTCTTTTT
AACAGATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGTCGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGAAA
ATAAGTTTGTAACTCTTAAATTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
CATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATTATGGCATTGATTACTGGTGA
CGTAAAAGAAATTAATGAGCAATTTAAGGAACGTGTTAAAGAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGC
ATATAGCTGCAATTGTATTCTTAATTTACCAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
ATCATTGTATTAACTTTATATGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCTATAATAATGACAACTCTTGT
ACTGCTTATTACTAAGCAAATTAAAATAAAGGGTATTCAGCTATTAGCATTTGCATTTATAATTATGTTTATTTTAAACC
CACTAGTTGTTTATGATATTGGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTTATCGTTGCCATTCCAAACTT
TCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATTTTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTA
TTTTATTCTTTATTACAAGTCATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
GACTGGTTACTAGACCTATTCACAAGAATTAAGCAATCACATTTTTCTGTTCCCAAGTTTAATGATTGGATATTTATAAT
ATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCTAAACGTAAATATATATTGGTTACGTTTTGGACTATAATTA
TTCTGACATTATTAATAACGTTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
TTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGATGATACTAAACAACCTAGTTA
TTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAAAGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATC
CACACAATGACCATATTGGTGAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAATAAGGGA
TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTTATGGATGTAAGACAAGTTAG
TAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGATAGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGA
TTATTACTATGATTACATATCAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGCAAGACAAGTAGTTCTAAAGAATTTAT
AGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGGAAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTA
AACGATTGCAAAGGATTCGCAGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
AAAGTTGATTCAAACTCTTATGGAAATGCAAGTGGTTTATAG

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(474-679)

Competence protein (Competence)

(183-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6K1MRM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Staphylococcus aureus MW2

98.772

100

0.988

Multiple sequence alignment    



References

[1] Mais Maree et al. (2022) Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. Nature Communications 13(1):2477. [PMID: 35513365]
[2] Annette Fagerlund et al. (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Molecular Microbiology 94(3):557-79. [PMID: 25155269]
[3] Kazuya Morikawa et al. (2003) A new staphylococcal sigma factor in the conserved gene cassette: functional significance and implication for the evolutionary processes. Genes To Cells : Devoted To Molecular & Cellular Mechanisms 8(8):699-712. [PMID: 12875655]