Detailed information    

experimental Experimentally validated

Overview

Name   comEA   Type   Machinery gene
Locus tag   GCO85_RS04230 Genome accession   NZ_CP048618
Coordinates   937853..938170 (+) Length   105 a.a.
NCBI ID   WP_010946546.1    Uniprot ID   Q5ZXC2
Organism   Legionella pneumophila strain ERS1305867     
Function   dsDNA binding   
DNA binding and uptake

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 932853..943170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS04210 (GCO85_04220) - 933005..934444 (+) 1440 WP_010946542.1 cation:proton antiporter -
  GCO85_RS04215 (GCO85_04225) nadC 934441..935283 (+) 843 WP_027226257.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GCO85_RS04220 (GCO85_04230) - 935280..936371 (+) 1092 WP_010946544.1 undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase -
  GCO85_RS04225 (GCO85_04235) - 936346..937761 (+) 1416 WP_010946545.1 M20 family metallopeptidase -
  GCO85_RS04230 (GCO85_04240) comEA 937853..938170 (+) 318 WP_010946546.1 helix-hairpin-helix domain-containing protein Machinery gene
  GCO85_RS04235 (GCO85_04245) - 938366..939403 (+) 1038 WP_011213320.1 rod shape-determining protein -
  GCO85_RS04240 (GCO85_04250) mreC 939381..940289 (+) 909 WP_010946548.1 rod shape-determining protein MreC -
  GCO85_RS04245 (GCO85_04255) mreD 940286..940765 (+) 480 WP_010946549.1 rod shape-determining protein MreD -
  GCO85_RS04250 (GCO85_04260) - 940701..941303 (-) 603 WP_061637143.1 pseudouridine synthase -
  GCO85_RS04255 (GCO85_04265) - 941564..942262 (+) 699 WP_072356110.1 protein tyrosine phosphatase -

Sequence

Protein


Download         Length: 105 a.a.        Molecular weight: 11773.78 Da        Isoelectric Point: 10.5233

>NTDB_id=1249 GCO85_RS04230 WP_010946546.1 937853..938170(+) (comEA) [Legionella pneumophila strain ERS1305867]
MKARLIAILLSFIVIPFSSQAENEPQKTPAKQVLVKGKINLNTADVYSLTGSFKGIGKKRAEAIIAYRENHQGFKSLEEL
AEVKGIGQRFVDKNREKLKEIFVIN

Nucleotide


Download         Length: 318 bp        

>NTDB_id=1249 GCO85_RS04230 WP_010946546.1 937853..938170(+) (comEA) [Legionella pneumophila strain ERS1305867]
ATGAAAGCAAGACTCATTGCTATTTTGTTGTCATTTATTGTTATCCCTTTCTCTTCTCAAGCTGAGAACGAGCCTCAAAA
AACTCCAGCTAAACAAGTCCTTGTTAAGGGCAAGATTAATCTTAATACAGCGGATGTGTATTCTTTAACAGGCTCTTTTA
AAGGGATAGGAAAAAAACGGGCTGAAGCTATTATTGCTTATAGAGAGAATCATCAAGGGTTTAAATCTCTGGAAGAGCTT
GCGGAAGTCAAGGGTATAGGGCAGCGTTTTGTAGATAAAAATAGAGAAAAACTGAAAGAAATTTTCGTTATTAATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(37-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZXC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Legionella pneumophila str. Paris

100

100

1
  comE1/comEA Haemophilus influenzae Rd KW20

35.455

100

0.371
  comEA Staphylococcus aureus MW2

38.384

94.286

0.362
  comEA Staphylococcus aureus N315

38.384

94.286

0.362

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]