Detailed information    

experimental Experimentally validated

Overview

Name   comE1/comEA   Type   Machinery gene
Locus tag   HI_1008 Genome accession   L42023
Coordinates   1073118..1073456 (+) Length   112 a.a.
NCBI ID   AAC22669.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   dsDNA binding   
DNA binding and uptake

Function

The deletion of comE1 reduced DNA uptake and transformation 6- and 10-fold, respectively.


Genomic Context

Location: 1068118..1078456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_1005 - 1069855..1071414 (+) 1560 AAC22666.1 conserved hypothetical protein -
  HI_1006 - 1071484..1071999 (+) 516 AAC22667.1 lipoprotein signal peptidase (lspA) -
  HI_1007 - 1071996..1072940 (+) 945 AAC22668.1 penicillin tolerance protein (lytB) -
  HI_1008 comE1/comEA 1073118..1073456 (+) 339 AAC22669.1 conserved hypothetical protein Machinery gene
  HI_1009 - 1073504..1074274 (-) 771 AAC22670.1 glycerol-3-phosphate regulon repressor (glpR) -
  HI_1010 - 1074433..1075338 (+) 906 AAC22671.1 3-hydroxyisobutyrate dehydrogenase, putative -
  HI_1011 - 1075341..1076582 (+) 1242 AAC22672.1 conserved hypothetical protein -
  HI_1012 - 1076579..1077211 (+) 633 AAC22673.1 sugar isomerase, putative -
  HI_1013 - 1077214..1077990 (+) 777 AAC22674.1 conserved hypothetical protein -

Sequence

Protein


Download         Length: 112 a.a.        Molecular weight: 11990.19 Da        Isoelectric Point: 8.0101

>NTDB_id=1362 HI_1008 AAC22669.1 1073118..1073456(+) (comE1/comEA) [Haemophilus influenzae Rd KW20]
MKTLFTSVVLCGALVVSSSFAEEKATXQTAQSVVTTQAEAQVAPAVVSDKLNINTATASEIQKSLTGIGAKKAEAIVQYR
EKHGNFXNAEQLLEVQGIGKATLEKNRDRIIF

Nucleotide


Download         Length: 339 bp        

>NTDB_id=1362 HI_1008 AAC22669.1 1073118..1073456(+) (comE1/comEA) [Haemophilus influenzae Rd KW20]
ATGAAAACATTATTCACTTCGGTTGTATTGTGTGGTGCGCTGGTTGTTTCTTCGTCTTTTGCTGAGGAAAAAGCGACAGA
NCAAACCGCTCAATCTGTTGTGACAACTCAAGCTGAAGCTCAAGTAGCACCAGCCGTAGTGAGCGATAAATTGAATATCA
ACACAGCAACTGCCAGTGAAATTCAAAAATCCTTAACTGGCATTGGTGCGAAAAAAGCGGAAGCTATTGTGCAATATCGT
GAAAAACACGGTAATTTTKCTAATGCAGAACAGCTTTTAGAAGTACAAGGAATTGGCAAAGCAACACTAGAGAAAAATCG
TGATCGTATAATCTTTTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(49-110)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/comE1 Glaesserella parasuis strain SC1401

58.261

100

0.598
  comEA Vibrio campbellii strain DS40M4

39.286

100

0.463
  comEA Vibrio cholerae strain A1552

40.541

100

0.437
  comEA Vibrio cholerae C6706

40.541

100

0.437
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

50.847

74.684

0.38
  comEA Vibrio parahaemolyticus RIMD 2210633

56.452

65.957

0.372
  comEA Legionella pneumophila str. Paris

35.455

100

0.371
  comEA Legionella pneumophila strain ERS1305867

35.455

100

0.371

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]