Detailed information    

experimental Experimentally validated

Overview

Name   comEA/comE1   Type   Machinery gene
Locus tag   A4U84_RS07375 Genome accession   NZ_CP015099
Coordinates   1551204..1551545 (-) Length   113 a.a.
NCBI ID   WP_062924234.1    Uniprot ID   -
Organism   Glaesserella parasuis strain SC1401     
Function   dsDNA binding   
DNA binding and uptake

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 1546204..1556545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS07345 (A4U84_07345) - 1547028..1547555 (-) 528 WP_043895844.1 DUF1523 family protein -
  A4U84_RS07350 (A4U84_07350) - 1547595..1548377 (-) 783 WP_016527622.1 TatD family hydrolase -
  A4U84_RS07355 (A4U84_07355) - 1548391..1548888 (-) 498 WP_021112650.1 GNAT family N-acetyltransferase -
  A4U84_RS07360 (A4U84_07360) - 1548889..1549167 (-) 279 WP_005714674.1 DUF1778 domain-containing protein -
  A4U84_RS07365 (A4U84_07365) epmA 1549221..1550201 (-) 981 WP_035495957.1 elongation factor P--(R)-beta-lysine ligase -
  A4U84_RS07370 (A4U84_07370) - 1550341..1551104 (+) 764 WP_106406633.1 IS5 family transposase -
  A4U84_RS07375 (A4U84_07375) comEA/comE1 1551204..1551545 (-) 342 WP_062924234.1 ComEA family DNA-binding protein Machinery gene
  A4U84_RS07380 (A4U84_07380) - 1551803..1553053 (+) 1251 WP_062924235.1 LysM-like peptidoglycan-binding domain-containing protein -
  A4U84_RS07385 (A4U84_07385) - 1553155..1553511 (+) 357 WP_021111031.1 MliC family protein -
  A4U84_RS07390 (A4U84_07390) waaF 1553520..1554572 (+) 1053 WP_062924236.1 lipopolysaccharide heptosyltransferase II -
  A4U84_RS07395 (A4U84_07395) - 1554668..1555255 (+) 588 WP_035494230.1 FMN-dependent NADH-azoreductase -

Sequence

Protein


Download         Length: 113 a.a.        Molecular weight: 12394.24 Da        Isoelectric Point: 8.9615

>NTDB_id=1382 A4U84_RS07375 WP_062924234.1 1551204..1551545(-) (comEA/comE1) [Glaesserella parasuis strain SC1401]
MKKLSRYTFSLLFGLFSATTFAEEKVTTSTEHTPVVMQQVAATQKVNANVVNINTATASEIQDKLVGIGAKKAQAIVEYR
EKNGKFLNVEQLTEVSGIGKATLEKNRDCIVIE

Nucleotide


Download         Length: 342 bp        

>NTDB_id=1382 A4U84_RS07375 WP_062924234.1 1551204..1551545(-) (comEA/comE1) [Glaesserella parasuis strain SC1401]
ATGAAAAAACTTTCTCGTTACACATTCAGTCTTTTATTTGGGCTTTTCTCTGCAACAACATTTGCTGAAGAAAAAGTAAC
TACGTCAACAGAACATACACCGGTAGTGATGCAACAAGTCGCAGCGACACAAAAAGTAAATGCGAATGTTGTAAATATCA
ATACTGCGACAGCCTCAGAAATCCAAGATAAGTTAGTCGGTATTGGAGCGAAAAAGGCTCAGGCAATTGTGGAATATCGT
GAGAAAAACGGTAAGTTTTTAAATGTTGAGCAGCTCACCGAAGTTTCAGGTATCGGGAAAGCGACACTTGAAAAAAACCG
AGATTGTATTGTGATTGAATAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(46-109)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE1/comEA Haemophilus influenzae Rd KW20

58.261

100

0.598
  Cj0011c Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

49.206

79.747

0.392
  comEA Vibrio parahaemolyticus RIMD 2210633

56.452

65.957

0.372
  comEA Vibrio campbellii strain DS40M4

56.452

65.263

0.368

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]