Detailed information    

experimental Experimentally validated

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   KZH43_RS09255 Genome accession   NZ_CP079923
Coordinates   1824733..1825059 (-) Length   108 a.a.
NCBI ID   WP_000738626.1    Uniprot ID   Q8DN88
Organism   Streptococcus pneumoniae Rx1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGC is essential for the assembly and function of the competence pseudopilus, a structure critical for DNA uptake during natural transformation.


Genomic Context

Location: 1819733..1830059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS09215 (KZH43_09210) rnpA 1819750..1820121 (-) 372 WP_000739246.1 ribonuclease P protein component -
  KZH43_RS10715 - 1820138..1820269 (-) 132 WP_000768904.1 hypothetical protein -
  KZH43_RS09220 (KZH43_09215) - 1820270..1821460 (-) 1191 WP_000167757.1 acetate kinase -
  KZH43_RS09225 (KZH43_09220) comYH 1821511..1822464 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  KZH43_RS09230 (KZH43_09225) - 1822525..1823119 (-) 595 Protein_1826 class I SAM-dependent methyltransferase -
  KZH43_RS09235 (KZH43_09230) comGG/cglG 1823256..1823669 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  KZH43_RS09240 (KZH43_09235) comGF/cglF 1823647..1824108 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  KZH43_RS09245 (KZH43_09240) comGE/cglE 1824071..1824373 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  KZH43_RS09250 (KZH43_09245) comGD/cglD 1824336..1824740 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  KZH43_RS09255 (KZH43_09250) comGC/cglC 1824733..1825059 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  KZH43_RS09260 (KZH43_09255) comGB/cglB 1825061..1826077 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  KZH43_RS09265 (KZH43_09260) comGA/cglA/cilD 1826025..1826966 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  KZH43_RS09270 (KZH43_09265) - 1827042..1827407 (-) 366 WP_000286415.1 DUF1033 family protein -
  KZH43_RS09275 (KZH43_09270) - 1827558..1828616 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  KZH43_RS09280 (KZH43_09275) nagA 1828779..1829930 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 108 a.a.        Molecular weight: 12200.38 Da        Isoelectric Point: 10.1877

>NTDB_id=272 KZH43_RS09255 WP_000738626.1 1824733..1825059(-) (comGC/cglC) [Streptococcus pneumoniae Rx1]
MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQA
DGRITEEQAKAYKEYHDKNGGANRKVND

Nucleotide


Download         Length: 327 bp        

>NTDB_id=272 KZH43_RS09255 WP_000738626.1 1824733..1825059(-) (comGC/cglC) [Streptococcus pneumoniae Rx1]
ATGAAAAAAATGATGACATTCTTGAAAAAAGCTAAGGTTAAAGCTTTTACATTGGTGGAGATGTTGGTGGTCTTGCTGAT
TATCAGCGTGCTTTTCTTGCTCTTTGTACCTAATCTGACCAAGCAAAAAGAAGCAGTCAATGACAAAGGAAAAGCAGCTG
TTGTTAAGGTGGTGGAAAGCCAGGCAGAACTTTATAGCTTAGAAAAGAATGAAGATGCTAGCCTAAGAAAGTTACAAGCA
GATGGACGCATCACGGAAGAACAGGCTAAAGCTTATAAAGAATACCATGATAAAAATGGAGGAGCAAATCGTAAAGTCAA
TGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6Y1H

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae R6

100

100

1
  comGC/cglC Streptococcus pneumoniae D39

100

100

1
  comGC/cglC Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comGC/cglC Streptococcus mitis SK321

94.444

100

0.944
  comGC/cglC Streptococcus mitis NCTC 12261

92.157

95.327

0.879
  comYC Streptococcus gordonii str. Challis substr. CH1

67.961

98.095

0.667
  comYC Streptococcus suis isolate S10

70.37

87.097

0.613
  comYC Streptococcus mutans UA140

63.636

95.192

0.606
  comYC Streptococcus mutans UA159

63.636

95.192

0.606
  comGC Lactococcus lactis subsp. cremoris KW2

53.922

94.444

0.509
  comGC Staphylococcus aureus MW2

46.078

99.029

0.456
  comGC Staphylococcus aureus N315

46.078

99.029

0.456
  comGC Latilactobacillus sakei subsp. sakei 23K

45.349

86.869

0.394

Multiple sequence alignment    



References

[1] Trinh Lam et al. (2021) Competence pili in Streptococcus pneumoniae are highly dynamic structures that retract to promote DNA uptake. Molecular Microbiology 116(2):381-396. [PMID: 33754381]
[2] Vitor Oliveira et al. (2021) The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae. Frontiers in Cellular And Infection Microbiology 11:808601. [PMID: 35004361]
[3] Devon Sheppard et al. (2020) The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold. The Journal of Biological Chemistry 295(19):6594-6604. [PMID: 32273343]
[4] Sandra Muschiol et al. (2017) Structure of the competence pilus major pilin ComGC in Streptococcus pneumoniae. The Journal of Biological Chemistry 292(34):14134-14146. [PMID: 28659339]
[5] Sandra Muschiol et al. (2015) Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae. BioEssays : News And Reviews in Molecular, Cellular And Developmental Biology 37(4):426-35. [PMID: 25640084]
[6] Raphaël Laurenceau et al. (2013) A type IV pilus mediates DNA binding during natural transformation in Streptococcus pneumoniae. PLoS Pathogens 9(6):e1003473. [PMID: 23825953]
[7] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]