Detailed information    

experimental Experimentally validated

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   KZH43_RS09240 Genome accession   NZ_CP079923
Coordinates   1823647..1824108 (-) Length   153 a.a.
NCBI ID   WP_000250534.1    Uniprot ID   -
Organism   Streptococcus pneumoniae Rx1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGF is a component of the competence pseudopilus machinery.


Genomic Context

Location: 1818647..1829108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS09210 (KZH43_09205) - 1818951..1819775 (-) 825 WP_220041240.1 membrane protein insertase YidC -
  KZH43_RS09215 (KZH43_09210) rnpA 1819750..1820121 (-) 372 WP_000739246.1 ribonuclease P protein component -
  KZH43_RS10715 - 1820138..1820269 (-) 132 WP_000768904.1 hypothetical protein -
  KZH43_RS09220 (KZH43_09215) - 1820270..1821460 (-) 1191 WP_000167757.1 acetate kinase -
  KZH43_RS09225 (KZH43_09220) comYH 1821511..1822464 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  KZH43_RS09230 (KZH43_09225) - 1822525..1823119 (-) 595 Protein_1826 class I SAM-dependent methyltransferase -
  KZH43_RS09235 (KZH43_09230) comGG/cglG 1823256..1823669 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  KZH43_RS09240 (KZH43_09235) comGF/cglF 1823647..1824108 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  KZH43_RS09245 (KZH43_09240) comGE/cglE 1824071..1824373 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  KZH43_RS09250 (KZH43_09245) comGD/cglD 1824336..1824740 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  KZH43_RS09255 (KZH43_09250) comGC/cglC 1824733..1825059 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  KZH43_RS09260 (KZH43_09255) comGB/cglB 1825061..1826077 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  KZH43_RS09265 (KZH43_09260) comGA/cglA/cilD 1826025..1826966 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  KZH43_RS09270 (KZH43_09265) - 1827042..1827407 (-) 366 WP_000286415.1 DUF1033 family protein -
  KZH43_RS09275 (KZH43_09270) - 1827558..1828616 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17972.67 Da        Isoelectric Point: 9.6092

>NTDB_id=275 KZH43_RS09240 WP_000250534.1 1823647..1824108(-) (comGF/cglF) [Streptococcus pneumoniae Rx1]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=275 KZH43_RS09240 WP_000250534.1 1823647..1824108(-) (comGF/cglF) [Streptococcus pneumoniae Rx1]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

100

100

1
  comGF/cglF Streptococcus pneumoniae R6

100

100

1
  comGF/cglF Streptococcus pneumoniae D39

100

100

1
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909
  comGF/cglF Streptococcus mitis NCTC 12261

89.542

100

0.895
  comGF Lactococcus lactis subsp. cremoris KW2

37.857

99.291

0.376
  comYF Streptococcus mutans UA140

40.299

93.056

0.375
  comYF Streptococcus mutans UA159

40.299

93.056

0.375

Multiple sequence alignment    



References

[1] Vitor Oliveira et al. (2021) The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae. Frontiers in Cellular And Infection Microbiology 11:808601. [PMID: 35004361]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]