Detailed information    

experimental Experimentally validated

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SM12261_RS00970 Genome accession   NZ_CP028414
Coordinates   194443..194904 (+) Length   153 a.a.
NCBI ID   WP_000250509.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 189443..199904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00935 (SM12261_0181) - 189939..190997 (+) 1059 WP_000649460.1 zinc-dependent alcohol dehydrogenase family protein -
  SM12261_RS00940 (SM12261_0182) - 191147..191512 (+) 366 WP_000286405.1 DUF1033 family protein -
  SM12261_RS00945 (SM12261_0183) comGA/cglA/cilD 191588..192529 (+) 942 WP_000249545.1 competence type IV pilus ATPase ComGA Machinery gene
  SM12261_RS00950 (SM12261_0184) comGB/cglB 192477..193493 (+) 1017 WP_004238896.1 competence type IV pilus assembly protein ComGB Machinery gene
  SM12261_RS00955 (SM12261_0185) comGC/cglC 193495..193818 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  SM12261_RS00960 (SM12261_0186) comGD/cglD 193781..194215 (+) 435 WP_029689647.1 competence type IV pilus minor pilin ComGD Machinery gene
  SM12261_RS00965 (SM12261_0187) comGE/cglE 194178..194480 (+) 303 WP_045597458.1 competence type IV pilus minor pilin ComGE Machinery gene
  SM12261_RS00970 (SM12261_0188) comGF/cglF 194443..194904 (+) 462 WP_000250509.1 competence type IV pilus minor pilin ComGF Machinery gene
  SM12261_RS00975 (SM12261_0189) comGG/cglG 194882..195295 (+) 414 WP_000265657.1 competence type IV pilus minor pilin ComGG Machinery gene
  SM12261_RS00980 (SM12261_0190) - 195328..195915 (+) 588 WP_000679792.1 class I SAM-dependent methyltransferase -
  SM12261_RS00985 (SM12261_0191) comYH 195976..196929 (+) 954 WP_000345104.1 class I SAM-dependent methyltransferase Machinery gene
  SM12261_RS00990 (SM12261_0192) - 196979..198169 (+) 1191 WP_000167778.1 acetate kinase -
  SM12261_RS00995 (SM12261_0193) rnpA 198319..198690 (+) 372 WP_000739243.1 ribonuclease P protein component -
  SM12261_RS01000 (SM12261_0194) - 198665..199489 (+) 825 WP_004238894.1 membrane protein insertase YidC -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17825.48 Da        Isoelectric Point: 8.8053

>NTDB_id=505 SM12261_RS00970 WP_000250509.1 194443..194904(+) (comGF/cglF) [Streptococcus mitis NCTC 12261]
MVQNSCWLSKSHKIKAFTLLESLIALIVISGSLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGD
RIYMKQDGKEIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEENQVVRFRFQFQKGLEREFIYRVEKAKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=505 SM12261_RS00970 WP_000250509.1 194443..194904(+) (comGF/cglF) [Streptococcus mitis NCTC 12261]
ATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAAAATCAAGGCTTTTACCCTGTTAGAATCCCTGATTGCCCTCAT
TGTCATCAGTGGAAGTTTACTCCTCTTTCAGGCCATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAAGAGTGGCTCTTGTTTGTGGACCAGCTGGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCGAT
CGCATTTATATGAAGCAGGATGGCAAGGAAATTGCGATAGGTAAGTCTAAATCGGACGATTTTCGAAAAACCGATGCCAG
TGGACGAGGCTATCAGCCTATGGTTTATGGTCTCAAATCCGCACAGATTACAGAGGAAAATCAAGTGGTTCGTTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis SK321

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

89.542

100

0.895
  comGF/cglF Streptococcus pneumoniae R6

89.542

100

0.895
  comGF/cglF Streptococcus pneumoniae TIGR4

89.542

100

0.895
  comGF/cglF Streptococcus pneumoniae Rx1

89.542

100

0.895
  comGF Lactococcus lactis subsp. cremoris KW2

39.286

99.291

0.39
  comYF Streptococcus mutans UA140

38.806

93.056

0.361
  comYF Streptococcus mutans UA159

38.806

93.056

0.361

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]