Detailed information NTG0000504

Experimentally validated

Overview

• Name: comGE/cglE
• Type: Machinery gene
• Locus tag: SM12261_RS00965
• Genome accession: NZ_CP028414
• Coordinates: 194178..194480 (+)
• Length: 100 a.a.
• NCBI ID: WP_045597458.1
• Uniprot ID: -
• Organism: Streptococcus mitis NCTC 12261
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 189178..199480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM12261_RS00935 (SM12261_0181)	-	189939..190997 (+)	1059	WP_000649460.1	zinc-dependent alcohol dehydrogenase family protein	-
SM12261_RS00940 (SM12261_0182)	-	191147..191512 (+)	366	WP_000286405.1	DUF1033 family protein	-
SM12261_RS00945 (SM12261_0183)	comGA/cglA/cilD	191588..192529 (+)	942	WP_000249545.1	competence type IV pilus ATPase ComGA	Machinery gene
SM12261_RS00950 (SM12261_0184)	comGB/cglB	192477..193493 (+)	1017	WP_004238896.1	competence type IV pilus assembly protein ComGB	Machinery gene
SM12261_RS00955 (SM12261_0185)	comGC/cglC	193495..193818 (+)	324	WP_000738641.1	comG operon protein ComGC	Machinery gene
SM12261_RS00960 (SM12261_0186)	comGD/cglD	193781..194215 (+)	435	WP_029689647.1	competence type IV pilus minor pilin ComGD	Machinery gene
SM12261_RS00965 (SM12261_0187)	comGE/cglE	194178..194480 (+)	303	WP_045597458.1	competence type IV pilus minor pilin ComGE	Machinery gene
SM12261_RS00970 (SM12261_0188)	comGF/cglF	194443..194904 (+)	462	WP_000250509.1	competence type IV pilus minor pilin ComGF	Machinery gene
SM12261_RS00975 (SM12261_0189)	comGG/cglG	194882..195295 (+)	414	WP_000265657.1	competence type IV pilus minor pilin ComGG	Machinery gene
SM12261_RS00980 (SM12261_0190)	-	195328..195915 (+)	588	WP_000679792.1	class I SAM-dependent methyltransferase	-
SM12261_RS00985 (SM12261_0191)	comYH	195976..196929 (+)	954	WP_000345104.1	class I SAM-dependent methyltransferase	Machinery gene
SM12261_RS00990 (SM12261_0192)	-	196979..198169 (+)	1191	WP_000167778.1	acetate kinase	-
SM12261_RS00995 (SM12261_0193)	rnpA	198319..198690 (+)	372	WP_000739243.1	ribonuclease P protein component	-

Sequence

Protein

• Length: 100 a.a.
• Molecular weight: 11149.14 Da
• Isoelectric Point: 9.7273

>NTDB_id=504 SM12261_RS00965 WP_045597458.1 194178..194480(+) (comGE/cglE) [Streptococcus mitis NCTC 12261]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEILRVAKMALQTGQNQVNINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide

• Length: 303 bp

>NTDB_id=504 SM12261_RS00965 WP_045597458.1 194178..194480(+) (comGE/cglE) [Streptococcus mitis NCTC 12261]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAGATTAGGGCAGTGATTTTACTGGAAGCAGTAGTCGCTTTAGCTATCTT
TGCCAGCATTGCGACCCTTCTTTTGGGACAAATTCAGAAAAATAGGCAGGAAGAAGCAAAAATCTTGCAGAAAGAAGAGA
TCTTGCGTGTAGCTAAGATGGCCTTGCAGACAGGTCAAAATCAGGTAAACATCAACGGAGTTGAGATTCAGGTGTTTTCT
AGTGAAAAGGGATTGGAGGTCTACCATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

• Type II secretory pathway pseudopilin (ComGE) (16-97)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGE/cglE	Streptococcus mitis SK321	99	100	0.99
comGE/cglE	Streptococcus pneumoniae D39	98	100	0.98
comGE/cglE	Streptococcus pneumoniae R6	98	100	0.98
comGE/cglE	Streptococcus pneumoniae TIGR4	98	100	0.98
comGE/cglE	Streptococcus pneumoniae Rx1	98	100	0.98
comYE	Streptococcus mutans UA159	44.444	92.784	0.412
comYE	Streptococcus mutans UA140	44.444	92.784	0.412

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]