Detailed information    

experimental Experimentally validated

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SM12261_RS00950 Genome accession   NZ_CP028414
Coordinates   192477..193493 (+) Length   338 a.a.
NCBI ID   WP_004238896.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 187477..198493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00930 (SM12261_0180) nagA 188625..189776 (+) 1152 WP_001134449.1 N-acetylglucosamine-6-phosphate deacetylase -
  SM12261_RS00935 (SM12261_0181) - 189939..190997 (+) 1059 WP_000649460.1 zinc-dependent alcohol dehydrogenase family protein -
  SM12261_RS00940 (SM12261_0182) - 191147..191512 (+) 366 WP_000286405.1 DUF1033 family protein -
  SM12261_RS00945 (SM12261_0183) comGA/cglA/cilD 191588..192529 (+) 942 WP_000249545.1 competence type IV pilus ATPase ComGA Machinery gene
  SM12261_RS00950 (SM12261_0184) comGB/cglB 192477..193493 (+) 1017 WP_004238896.1 competence type IV pilus assembly protein ComGB Machinery gene
  SM12261_RS00955 (SM12261_0185) comGC/cglC 193495..193818 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  SM12261_RS00960 (SM12261_0186) comGD/cglD 193781..194215 (+) 435 WP_029689647.1 competence type IV pilus minor pilin ComGD Machinery gene
  SM12261_RS00965 (SM12261_0187) comGE/cglE 194178..194480 (+) 303 WP_045597458.1 competence type IV pilus minor pilin ComGE Machinery gene
  SM12261_RS00970 (SM12261_0188) comGF/cglF 194443..194904 (+) 462 WP_000250509.1 competence type IV pilus minor pilin ComGF Machinery gene
  SM12261_RS00975 (SM12261_0189) comGG/cglG 194882..195295 (+) 414 WP_000265657.1 competence type IV pilus minor pilin ComGG Machinery gene
  SM12261_RS00980 (SM12261_0190) - 195328..195915 (+) 588 WP_000679792.1 class I SAM-dependent methyltransferase -
  SM12261_RS00985 (SM12261_0191) comYH 195976..196929 (+) 954 WP_000345104.1 class I SAM-dependent methyltransferase Machinery gene
  SM12261_RS00990 (SM12261_0192) - 196979..198169 (+) 1191 WP_000167778.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38362.31 Da        Isoelectric Point: 9.4588

>NTDB_id=501 SM12261_RS00950 WP_004238896.1 192477..193493(+) (comGB/cglB) [Streptococcus mitis NCTC 12261]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRAGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVGVLLALTFYKRGSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDMAHALQNGQEFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=501 SM12261_RS00950 WP_004238896.1 192477..193493(+) (comGB/cglB) [Streptococcus mitis NCTC 12261]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
TAATCTCTTTTCCAGCGGTTTTCATCTGGTAGAGACTATCTCCTTTCTAGATAGGAGTGCCTTGTTGGACAAGCAGTGCG
TAACCCAGATGCGTGCGGGCTTATCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCAGAGGTTCATGGGAATCTACACCTGAGTTTGGGAAAGATAGAAGAATATCTGGATAATTT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGGTTTCTTCTCTTAATTATGCTGG
GGTTGCGTAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATTATTGGCAATCTGCCACAAATTTTT
CTAGGAATGGTAGGCTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAGGTTCGAAGATGCGGGTCTT
TTCTATCCTGGCACGCATTCCTTTTCTTGGAATTTTTGTGCAGACCTATCTGACAGCCTATTATGCGCGTGAATGGGGCA
ATATGATTTCTCAAGGGATGGAGTTGACGCAGATTTTTCAAATCATGCAGGAACAAGGTTCCCAGCTATTTAAAGAAATT
GGTCAAGATATGGCTCATGCCCTACAAAATGGTCAGGAATTTTCTCAAACCATAGGAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGTTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATCTGGTGCAGCCACTGGTCTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae D39

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae R6

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae TIGR4

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae Rx1

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

72.321

99.408

0.719
  comYB Streptococcus mutans UA140

58.407

98.834

0.577
  comYB Streptococcus mutans UA159

58.407

98.834

0.577
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]