Detailed information    

experimental Experimentally validated

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SMSK321_RS09235 Genome accession   NZ_AEDT01000028
Coordinates   20194..21210 (-) Length   338 a.a.
NCBI ID   WP_080550783.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 15194..26210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS09200 (SMSK321_1265) - 15514..16704 (-) 1191 WP_000167794.1 acetate kinase -
  SMSK321_RS09205 (SMSK321_1266) comYH 16755..17708 (-) 954 WP_000345131.1 class I SAM-dependent methyltransferase Machinery gene
  SMSK321_RS09210 (SMSK321_1267) - 17769..18356 (-) 588 WP_000679788.1 class I SAM-dependent methyltransferase -
  SMSK321_RS0102610 comGG/cglG 18389..18802 (-) 414 WP_000265640.1 competence type IV pilus minor pilin ComGG Machinery gene
  SMSK321_RS09215 (SMSK321_1268) comGF/cglF 18780..19241 (-) 462 WP_000250512.1 competence type IV pilus minor pilin ComGF Machinery gene
  SMSK321_RS09220 (SMSK321_1269) comGE/cglE 19204..19506 (-) 303 WP_255262223.1 competence type IV pilus minor pilin ComGE Machinery gene
  SMSK321_RS09225 (SMSK321_1270) comGD/cglD 19469..19873 (-) 405 WP_000588032.1 competence type IV pilus minor pilin ComGD Machinery gene
  SMSK321_RS09230 (SMSK321_1271) comGC/cglC 19866..20192 (-) 327 WP_000738643.1 comG operon protein ComGC Machinery gene
  SMSK321_RS09235 (SMSK321_1272) comGB/cglB 20194..21210 (-) 1017 WP_080550783.1 competence type IV pilus assembly protein ComGB Machinery gene
  SMSK321_RS09240 (SMSK321_1273) comGA/cglA/cilD 21158..22100 (-) 943 Protein_25 competence type IV pilus ATPase ComGA Machinery gene
  SMSK321_RS09245 (SMSK321_1274) - 22177..22542 (-) 366 WP_000286410.1 DUF1033 family protein -
  SMSK321_RS09250 (SMSK321_1275) - 22693..23751 (-) 1059 WP_004235578.1 zinc-dependent alcohol dehydrogenase family protein -
  SMSK321_RS09255 (SMSK321_1276) nagA 23914..25065 (-) 1152 WP_001134547.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38523.45 Da        Isoelectric Point: 9.4585

>NTDB_id=529 SMSK321_RS09235 WP_080550783.1 20194..21210(-) (comGB/cglB) [Streptococcus mitis SK321]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTDLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLSIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSLLTLLALTFYKRSSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQSLQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=529 SMSK321_RS09235 WP_080550783.1 20194..21210(-) (comGB/cglB) [Streptococcus mitis SK321]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTAGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTATTGGACAAGCAATGTG
TGACCCAGATGCGCACGGACTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTACTGGGATTTCTTCTCTCAATCATGCTGG
GGCTACGGAACTACCTACTACCACAACTGGATAGTAGCAATATTGCTACCCAAATTATCGGCAATCTGCCACAAATCTTT
CTAGGAATGGTAGGGTTGGTTTCTCTACTTACCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCCTAGCACGCATTCCTTTTCTTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCGCGTGAATGGGGTA
ATATGATTTCACAGGGGATGGAGTTGACGCAGATTTTTCAGATCATGCAAGAACAAGGCTCCCAGCTCTTTAAAGAAATC
GGTCAAGACCTAGCTCAATCCCTACAAAATGGCCGGGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAATCGCACCATGAATCTGGTACAGCCACTAGTCTTTATCTTTGTGGCTCTTATTATCGTT
TTACTTTACGCGGCAATGCTAATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae R6

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae Rx1

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae D39

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae TIGR4

95.266

100

0.953
  comYB Streptococcus gordonii str. Challis substr. CH1

72.619

99.408

0.722
  comYB Streptococcus mutans UA159

56.548

97.959

0.554
  comYB Streptococcus mutans UA140

56.548

97.959

0.554
  comGB Lactococcus lactis subsp. cremoris KW2

51.497

98.817

0.509

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]