Detailed information NTG0000531

Experimentally validated

Overview

• Name: comGD/cglD
• Type: Machinery gene
• Locus tag: SMSK321_RS09225
• Genome accession: NZ_AEDT01000028
• Coordinates: 19469..19873 (-)
• Length: 134 a.a.
• NCBI ID: WP_000588032.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 14469..24873

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS09195 (SMSK321_1264)	rnpA	14993..15364 (-)	372	WP_000739245.1	ribonuclease P protein component	-
SMSK321_RS09200 (SMSK321_1265)	-	15514..16704 (-)	1191	WP_000167794.1	acetate kinase	-
SMSK321_RS09205 (SMSK321_1266)	comYH	16755..17708 (-)	954	WP_000345131.1	class I SAM-dependent methyltransferase	Machinery gene
SMSK321_RS09210 (SMSK321_1267)	-	17769..18356 (-)	588	WP_000679788.1	class I SAM-dependent methyltransferase	-
SMSK321_RS0102610	comGG/cglG	18389..18802 (-)	414	WP_000265640.1	competence type IV pilus minor pilin ComGG	Machinery gene
SMSK321_RS09215 (SMSK321_1268)	comGF/cglF	18780..19241 (-)	462	WP_000250512.1	competence type IV pilus minor pilin ComGF	Machinery gene
SMSK321_RS09220 (SMSK321_1269)	comGE/cglE	19204..19506 (-)	303	WP_255262223.1	competence type IV pilus minor pilin ComGE	Machinery gene
SMSK321_RS09225 (SMSK321_1270)	comGD/cglD	19469..19873 (-)	405	WP_000588032.1	competence type IV pilus minor pilin ComGD	Machinery gene
SMSK321_RS09230 (SMSK321_1271)	comGC/cglC	19866..20192 (-)	327	WP_000738643.1	comG operon protein ComGC	Machinery gene
SMSK321_RS09235 (SMSK321_1272)	comGB/cglB	20194..21210 (-)	1017	WP_080550783.1	competence type IV pilus assembly protein ComGB	Machinery gene
SMSK321_RS09240 (SMSK321_1273)	comGA/cglA/cilD	21158..22100 (-)	943	Protein_25	competence type IV pilus ATPase ComGA	Machinery gene
SMSK321_RS09245 (SMSK321_1274)	-	22177..22542 (-)	366	WP_000286410.1	DUF1033 family protein	-
SMSK321_RS09250 (SMSK321_1275)	-	22693..23751 (-)	1059	WP_004235578.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 134 a.a.
• Molecular weight: 14624.79 Da
• Isoelectric Point: 10.2164

>NTDB_id=531 SMSK321_RS09225 WP_000588032.1 19469..19873(-) (comGD/cglD) [Streptococcus mitis SK321]
MIKAFTMLESLLVLGLVSIVALGLSGSVQSTFAAVEEQIFFMEFEELYRETQKRSVASQQKTSLNLDGQTISNGSQKLTV
PKGIQAPSGQSITFDRAGGNSSLAKVEFQTGKGTIRYQLYLGNGKIKRIKETKN

Nucleotide

• Length: 405 bp

>NTDB_id=531 SMSK321_RS09225 WP_000588032.1 19469..19873(-) (comGD/cglD) [Streptococcus mitis SK321]
ATGATTAAGGCCTTTACCATGCTGGAAAGCCTCTTAGTTTTGGGACTTGTGAGTATCGTTGCCTTGGGCTTGTCTGGCTC
TGTTCAGTCCACTTTTGCGGCGGTAGAGGAGCAGATTTTCTTTATGGAGTTTGAAGAACTCTATCGGGAAACCCAAAAAC
GCAGTGTAGCTAGTCAGCAAAAGACTAGTCTAAACTTAGATGGGCAGACGATTAGCAATGGCAGTCAAAAGTTGACAGTT
CCTAAAGGCATTCAAGCACCATCCGGCCAAAGTATTACATTTGACCGAGCTGGGGGCAATTCGTCCCTGGCTAAGGTTGA
ATTTCAGACCGGTAAAGGAACGATTCGTTATCAATTATATCTAGGAAATGGAAAAATTAAACGCATTAAGGAAACAAAAA
ATTAG

Domains

No domain identified.

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGD/cglD	Streptococcus pneumoniae Rx1	96.269	100	0.963
comGD/cglD	Streptococcus pneumoniae D39	96.269	100	0.963
comGD/cglD	Streptococcus pneumoniae R6	96.269	100	0.963
comGD/cglD	Streptococcus mitis NCTC 12261	96.241	99.254	0.955
comGD/cglD	Streptococcus pneumoniae TIGR4	95.522	100	0.955
comYD	Streptococcus gordonii str. Challis substr. CH1	59.055	94.776	0.56
comYD	Streptococcus mutans UA159	48.438	95.522	0.463
comYD	Streptococcus mutans UA140	48.438	95.522	0.463
comGD	Lactococcus lactis subsp. cremoris KW2	36.842	99.254	0.366

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]