Detailed information    

experimental Experimentally validated

Overview

Name   comGD/cglD   Type   Machinery gene
Locus tag   SP_RS10365 Genome accession   NC_003028
Coordinates   1951946..1952350 (-) Length   134 a.a.
NCBI ID   WP_000588028.1    Uniprot ID   -
Organism   Streptococcus pneumoniae TIGR4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGD is a component of the competence pseudopilus machinery.


Genomic Context

Location: 1946946..1957350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS10325 (SP_2042) rnpA 1947360..1947731 (-) 372 WP_000739253.1 ribonuclease P protein component -
  SP_RS13700 (SP_2043) - 1947748..1947879 (-) 132 WP_000768904.1 hypothetical protein -
  SP_RS10330 (SP_2044) - 1947880..1949070 (-) 1191 WP_000167757.1 acetate kinase -
  SP_RS10335 (SP_2045) comYH 1949121..1950074 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SP_RS12490 (SP_2046) - 1950135..1950729 (-) 595 Protein_1942 class I SAM-dependent methyltransferase -
  SP_RS10350 (SP_2047) comGG/cglG 1950866..1951279 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP_RS10355 (SP_2048) comGF/cglF 1951257..1951718 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP_RS10360 (SP_2049) comGE/cglE 1951681..1951983 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP_RS10365 (SP_2050) comGD/cglD 1951946..1952350 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP_RS10370 (SP_2051) comGC/cglC 1952343..1952669 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  SP_RS10375 (SP_2052) comGB/cglB 1952671..1953687 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP_RS10380 (SP_2053) comGA/cglA/cilD 1953635..1954576 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SP_RS10385 (SP_2054) - 1954652..1955017 (-) 366 WP_000286415.1 DUF1033 family protein -
  SP_RS10390 (SP_2055) - 1955168..1956226 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 14650.83 Da        Isoelectric Point: 10.2164

>NTDB_id=163 SP_RS10365 WP_000588028.1 1951946..1952350(-) (comGD/cglD) [Streptococcus pneumoniae TIGR4]
MIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVEEQIFFMEFEELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPV
PKGIQAPSGQSITFDRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN

Nucleotide


Download         Length: 405 bp        

>NTDB_id=163 SP_RS10365 WP_000588028.1 1951946..1952350(-) (comGD/cglD) [Streptococcus pneumoniae TIGR4]
ATGATTAAGGCCTTTACCATGCTGGAAAGTCTCTTGGTTTTGGGACTTGTGAGTATCCTTGCCTTGGGCTTGTCCGGCTC
TGTCCAGTCCACTTTTTCAGCGGTAGAGGAACAGATTTTCTTTATGGAGTTTGAAGAACTCTATCGGGAAACCCAAAAAC
GCAGTGTAGCCAGTCAGCAAAAGACTAGTCTGAACTTAGATGGGCAGACGCTTAGCAATGGCAGTCAAAAGTTGCCAGTC
CCTAAAGGAATTCAGGCCCCATCAGGCCAAAGTATTACATTTGACCGAGCTGGGGGCAATTCGTCCCTGGCTAAGGTTGA
ATTTCAGACCAGTAAAGGAGCGATTCGCTATCAATTATATCTAGGAAATGGAAAAATTAAACGCATTAAGGAAACAAAAA
ATTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD/cglD Streptococcus pneumoniae R6

97.761

100

0.978
  comGD/cglD Streptococcus pneumoniae D39

97.761

100

0.978
  comGD/cglD Streptococcus pneumoniae Rx1

97.761

100

0.978
  comGD/cglD Streptococcus mitis NCTC 12261

96.241

99.254

0.955
  comGD/cglD Streptococcus mitis SK321

95.522

100

0.955
  comYD Streptococcus gordonii str. Challis substr. CH1

59.055

94.776

0.56
  comYD Streptococcus mutans UA159

48.438

95.522

0.463
  comYD Streptococcus mutans UA140

48.438

95.522

0.463

Multiple sequence alignment    



References

[1] Vitor Oliveira et al. (2021) The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae. Frontiers in Cellular And Infection Microbiology 11:808601. [PMID: 35004361]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]