Detailed information    

experimental Experimentally validated

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   SP_RS10370 Genome accession   NC_003028
Coordinates   1952343..1952669 (-) Length   108 a.a.
NCBI ID   WP_000738627.1    Uniprot ID   A0A0H2URU9
Organism   Streptococcus pneumoniae TIGR4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGC is essential for the assembly and function of the competence pseudopilus, a structure critical for DNA uptake during natural transformation.


Genomic Context

Location: 1947343..1957669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS10325 (SP_2042) rnpA 1947360..1947731 (-) 372 WP_000739253.1 ribonuclease P protein component -
  SP_RS13700 (SP_2043) - 1947748..1947879 (-) 132 WP_000768904.1 hypothetical protein -
  SP_RS10330 (SP_2044) - 1947880..1949070 (-) 1191 WP_000167757.1 acetate kinase -
  SP_RS10335 (SP_2045) comYH 1949121..1950074 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SP_RS12490 (SP_2046) - 1950135..1950729 (-) 595 Protein_1942 class I SAM-dependent methyltransferase -
  SP_RS10350 (SP_2047) comGG/cglG 1950866..1951279 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP_RS10355 (SP_2048) comGF/cglF 1951257..1951718 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP_RS10360 (SP_2049) comGE/cglE 1951681..1951983 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP_RS10365 (SP_2050) comGD/cglD 1951946..1952350 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP_RS10370 (SP_2051) comGC/cglC 1952343..1952669 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  SP_RS10375 (SP_2052) comGB/cglB 1952671..1953687 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP_RS10380 (SP_2053) comGA/cglA/cilD 1953635..1954576 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SP_RS10385 (SP_2054) - 1954652..1955017 (-) 366 WP_000286415.1 DUF1033 family protein -
  SP_RS10390 (SP_2055) - 1955168..1956226 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  SP_RS10395 (SP_2056) nagA 1956389..1957540 (-) 1152 WP_001134456.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 108 a.a.        Molecular weight: 12177.34 Da        Isoelectric Point: 10.1876

>NTDB_id=162 SP_RS10370 WP_000738627.1 1952343..1952669(-) (comGC/cglC) [Streptococcus pneumoniae TIGR4]
MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKEAVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQA
DGRITEEQAKAYKEYNDKNGGANRKVND

Nucleotide


Download         Length: 327 bp        

>NTDB_id=162 SP_RS10370 WP_000738627.1 1952343..1952669(-) (comGC/cglC) [Streptococcus pneumoniae TIGR4]
ATGAAAAAAATGATGACATTCTTGAAAAAAGCTAAGGTTAAAGCTTTTACATTGGTGGAGATGTTGGTGGTCTTGCTGAT
TATCAGCGTGCTTTTCTTGCTCTTTGTACCTAATCTGACCAAGCAAAAAGAAGCAGTCAATGACAAAGGAAAAGCAGCTG
TTGTTAAGGTGGTGGAAAGCCAGGCAGAACTTTATAGCTTAGAAAAGAATGAAGATGCTAGCCTAAGAAAGTTACAAGCA
GATGGACGCATCACGGAAGAACAGGCTAAAGCTTATAAAGAATACAATGATAAAAATGGAGGAGCAAATCGTAAAGTCAA
TGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5NCA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae D39

99.074

100

0.991
  comGC/cglC Streptococcus pneumoniae R6

99.074

100

0.991
  comGC/cglC Streptococcus pneumoniae Rx1

99.074

100

0.991
  comGC/cglC Streptococcus mitis SK321

94.444

100

0.944
  comGC/cglC Streptococcus mitis NCTC 12261

90.291

96.262

0.869
  comYC Streptococcus gordonii str. Challis substr. CH1

67.961

98.095

0.667
  comYC Streptococcus suis isolate S10

70.37

87.097

0.613
  comYC Streptococcus mutans UA140

63.636

95.192

0.606
  comYC Streptococcus mutans UA159

63.636

95.192

0.606
  comGC Lactococcus lactis subsp. cremoris KW2

53.922

94.444

0.509
  comGC Staphylococcus aureus MW2

46.078

99.029

0.456
  comGC Staphylococcus aureus N315

46.078

99.029

0.456
  comGC Latilactobacillus sakei subsp. sakei 23K

45.349

86.869

0.394

Multiple sequence alignment    



References

[1] Trinh Lam et al. (2021) Competence pili in Streptococcus pneumoniae are highly dynamic structures that retract to promote DNA uptake. Molecular Microbiology 116(2):381-396. [PMID: 33754381]
[2] Vitor Oliveira et al. (2021) The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae. Frontiers in Cellular And Infection Microbiology 11:808601. [PMID: 35004361]
[3] Devon Sheppard et al. (2020) The major subunit of widespread competence pili exhibits a novel and conserved type IV pilin fold. The Journal of Biological Chemistry 295(19):6594-6604. [PMID: 32273343]
[4] Sandra Muschiol et al. (2017) Structure of the competence pilus major pilin ComGC in Streptococcus pneumoniae. The Journal of Biological Chemistry 292(34):14134-14146. [PMID: 28659339]
[5] Sandra Muschiol et al. (2015) Uptake of extracellular DNA: competence induced pili in natural transformation of Streptococcus pneumoniae. BioEssays : News And Reviews in Molecular, Cellular And Developmental Biology 37(4):426-35. [PMID: 25640084]
[6] Raphaël Laurenceau et al. (2013) A type IV pilus mediates DNA binding during natural transformation in Streptococcus pneumoniae. PLoS Pathogens 9(6):e1003473. [PMID: 23825953]
[7] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]