Detailed information    

experimental Experimentally validated

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SP_RS10375 Genome accession   NC_003028
Coordinates   1952671..1953687 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae TIGR4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

ComGB is a critical component of the DNA uptake machinery during natural transformation. It is required for the assembly of the competence pseudopilus, a structure essential for DNA binding and transport across the cell membrane.


Genomic Context

Location: 1947671..1958687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS13700 (SP_2043) - 1947748..1947879 (-) 132 WP_000768904.1 hypothetical protein -
  SP_RS10330 (SP_2044) - 1947880..1949070 (-) 1191 WP_000167757.1 acetate kinase -
  SP_RS10335 (SP_2045) comYH 1949121..1950074 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SP_RS12490 (SP_2046) - 1950135..1950729 (-) 595 Protein_1942 class I SAM-dependent methyltransferase -
  SP_RS10350 (SP_2047) comGG/cglG 1950866..1951279 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP_RS10355 (SP_2048) comGF/cglF 1951257..1951718 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP_RS10360 (SP_2049) comGE/cglE 1951681..1951983 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP_RS10365 (SP_2050) comGD/cglD 1951946..1952350 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP_RS10370 (SP_2051) comGC/cglC 1952343..1952669 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  SP_RS10375 (SP_2052) comGB/cglB 1952671..1953687 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP_RS10380 (SP_2053) comGA/cglA/cilD 1953635..1954576 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SP_RS10385 (SP_2054) - 1954652..1955017 (-) 366 WP_000286415.1 DUF1033 family protein -
  SP_RS10390 (SP_2055) - 1955168..1956226 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  SP_RS10395 (SP_2056) nagA 1956389..1957540 (-) 1152 WP_001134456.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=161 SP_RS10375 WP_074196785.1 1952671..1953687(-) (comGB/cglB) [Streptococcus pneumoniae TIGR4]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=161 SP_RS10375 WP_074196785.1 1952671..1953687(-) (comGB/cglB) [Streptococcus pneumoniae TIGR4]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA159

55.952

97.959

0.548
  comYB Streptococcus mutans UA140

55.952

97.959

0.548
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment    



References

[1] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]