NTDB

Overview

Name	comGA/cglA/cilD	Type	Machinery gene
Locus tag	SP_RS10380	Genome accession	NC_003028
Coordinates	1953635..1954576 (-)	Length	313 a.a.
NCBI ID	WP_000249564.1	Uniprot ID	Q8DN86
Organism	Streptococcus pneumoniae TIGR4
Function	dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

The comGA gene is essential for the initial binding of transforming DNA to the cell surface and for the assembly of the pseudopilus, a structure required for DNA uptake.

Genomic Context

Location: 1948635..1959576

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SP_RS10335 (SP_2045)	comYH	1949121..1950074 (-)	954	WP_000345135.1	class I SAM-dependent methyltransferase	Machinery gene
SP_RS12490 (SP_2046)	-	1950135..1950729 (-)	595	Protein_1942	class I SAM-dependent methyltransferase	-
SP_RS10350 (SP_2047)	comGG/cglG	1950866..1951279 (-)	414	WP_000265622.1	competence type IV pilus minor pilin ComGG	Machinery gene
SP_RS10355 (SP_2048)	comGF/cglF	1951257..1951718 (-)	462	WP_000250534.1	competence type IV pilus minor pilin ComGF	Machinery gene
SP_RS10360 (SP_2049)	comGE/cglE	1951681..1951983 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
SP_RS10365 (SP_2050)	comGD/cglD	1951946..1952350 (-)	405	WP_000588028.1	competence type IV pilus minor pilin ComGD	Machinery gene
SP_RS10370 (SP_2051)	comGC/cglC	1952343..1952669 (-)	327	WP_000738627.1	comG operon protein ComGC	Machinery gene
SP_RS10375 (SP_2052)	comGB/cglB	1952671..1953687 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
SP_RS10380 (SP_2053)	comGA/cglA/cilD	1953635..1954576 (-)	942	WP_000249564.1	competence type IV pilus ATPase ComGA	Machinery gene
SP_RS10385 (SP_2054)	-	1954652..1955017 (-)	366	WP_000286415.1	DUF1033 family protein	-
SP_RS10390 (SP_2055)	-	1955168..1956226 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
SP_RS10395 (SP_2056)	nagA	1956389..1957540 (-)	1152	WP_001134456.1	N-acetylglucosamine-6-phosphate deacetylase	-
SP_RS10400 (SP_2057)	-	1957693..1959510 (-)	1818	WP_001220853.1	acyltransferase family protein	-

Sequence

Protein

Download Length: 313 a.a. Molecular weight: 35555.47 Da Isoelectric Point: 6.1330

                                >NTDB_id=160 SP_RS10380 WP_000249564.1 1953635..1954576(-) (comGA/cglA/cilD) [Streptococcus pneumoniae TIGR4]

MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA

YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ

VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY

ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide

Download Length: 942 bp

                                >NTDB_id=160 SP_RS10380 WP_000249564.1 1953635..1954576(-) (comGA/cglA/cilD) [Streptococcus pneumoniae TIGR4]

ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT

AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG

TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC

TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT

GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT

ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA

GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC

CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG

CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT

GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG

GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC

TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8DN86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA/cglA/cilD	Streptococcus pneumoniae D39	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae R6	100	100	1
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	95.847	100	0.958
comYA	Streptococcus gordonii str. Challis substr. CH1	78.065	99.042	0.773
comYA	Streptococcus mutans UA140	65.916	99.361	0.655
comYA	Streptococcus mutans UA159	65.916	99.361	0.655
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	62.581	99.042	0.62
comGA	Lactococcus lactis subsp. cremoris KW2	54.808	99.681	0.546
comGA	Latilactobacillus sakei subsp. sakei 23K	41.912	91.892	0.385

Multiple sequence alignment

References

[1]	Cheng-Foh Le et al. (2016) Transcriptome analysis of Streptococcus pneumoniae treated with the designed antimicrobial peptides, DM3. Scientific Reports 6:26828. [PMID: 27225022]
[2]	Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[3]	Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
[4]	Ping Luo et al. (2003) ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae. Molecular Microbiology 50(2):623-33. [PMID: 14617184]
[5]	Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]

Detailed information NTG0000160