Detailed information    

experimental Experimentally validated

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   DLJ51_RS00890 Genome accession   NZ_CP029560
Coordinates   152832..153779 (+) Length   315 a.a.
NCBI ID   WP_019777327.1    Uniprot ID   -
Organism   Streptococcus sobrinus strain NIDR 6715-7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

XIP increases the expression of the comX, ssbB, and cglA genes in strain NIDR 6715-7.


Genomic Context

Location: 147832..158779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DLJ51_RS00875 (DLJ51_00875) - 148711..149073 (-) 363 WP_002959493.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  DLJ51_RS00880 (DLJ51_00880) - 149434..151818 (-) 2385 WP_019785320.1 HAD-IC family P-type ATPase -
  DLJ51_RS00885 (DLJ51_00885) - 152038..152406 (+) 369 WP_019781971.1 DUF1033 family protein -
  DLJ51_RS00890 (DLJ51_00890) comGA/cglA 152832..153779 (+) 948 WP_019777327.1 competence type IV pilus ATPase ComGA Machinery gene
  DLJ51_RS00895 (DLJ51_00895) comGB 153703..154746 (+) 1044 WP_002959504.1 competence type IV pilus assembly protein ComGB Machinery gene
  DLJ51_RS00900 (DLJ51_00900) comGC 154743..155069 (+) 327 WP_019770073.1 competence type IV pilus major pilin ComGC Machinery gene
  DLJ51_RS00905 (DLJ51_00905) comGD 155023..155457 (+) 435 WP_254051091.1 competence type IV pilus minor pilin ComGD Machinery gene
  DLJ51_RS00910 (DLJ51_00910) comGE 155429..155722 (+) 294 WP_002959510.1 competence type IV pilus minor pilin ComGE Machinery gene
  DLJ51_RS00915 (DLJ51_00915) comGF 155706..156143 (+) 438 WP_019781940.1 competence type IV pilus minor pilin ComGF Machinery gene
  DLJ51_RS00920 (DLJ51_00920) comGG 156121..156582 (+) 462 WP_019785527.1 competence type IV pilus minor pilin ComGG Machinery gene
  DLJ51_RS00930 (DLJ51_00930) - 156784..157050 (+) 267 WP_002959516.1 type II toxin-antitoxin system RelE/ParE family toxin -
  DLJ51_RS00935 (DLJ51_00935) - 157037..157312 (+) 276 WP_109982370.1 helix-turn-helix domain-containing protein -
  DLJ51_RS00940 (DLJ51_00940) comGH 157411..158364 (+) 954 WP_019771347.1 class I SAM-dependent methyltransferase Machinery gene

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comS comGA/cglA positive effect
  comS comX positive effect
  comS ssbB positive effect

Sequence

Protein


Download         Length: 315 a.a.        Molecular weight: 35332.67 Da        Isoelectric Point: 6.3082

>NTDB_id=584 DLJ51_RS00890 WP_019777327.1 152832..153779(+) (comGA/cglA) [Streptococcus sobrinus strain NIDR 6715-7]
MVEVQELGKELIRQAVEEQAQDIYLLPKPAVYKIYLRFGEKRHLIQTCPPEQAAGLISHFKFLAGMNVGEKRRAQLGACD
YAFADGETAALRLSTVGDYQGRESLVIRLLFDRKRELKFWFDGLNALLPKIIGRGLYLFSGPVGSGKTTLMYELVSRKYP
DSQIITIEDPVEIKQDNMLQLQLNPSIGMTYDHLIKLSLRHRPDVLIIGEIRDRETAQAVIRASLTGIRVFSTVHAKSIP
GVYARILELGVSQDELDNCLAGIAYQRLIGGGGLIDFAQDHFQNHQAECWNEKIDQLLAAGHLTEEEAAAEKIKD

Nucleotide


Download         Length: 948 bp        

>NTDB_id=584 DLJ51_RS00890 WP_019777327.1 152832..153779(+) (comGA/cglA) [Streptococcus sobrinus strain NIDR 6715-7]
ATGGTTGAAGTGCAGGAGCTGGGAAAGGAATTGATTAGACAGGCAGTTGAGGAGCAGGCTCAGGATATTTATCTTTTGCC
CAAGCCGGCTGTTTATAAGATTTATCTGCGGTTTGGCGAGAAGCGGCACTTGATTCAGACTTGCCCTCCCGAGCAGGCTG
CGGGTCTGATTAGTCACTTTAAATTTCTTGCTGGCATGAATGTTGGTGAGAAGCGACGGGCCCAGCTGGGTGCCTGTGAT
TATGCCTTCGCTGATGGTGAGACAGCGGCCTTGCGTTTATCGACGGTTGGCGACTATCAAGGGCGAGAAAGTCTGGTTAT
TCGCCTGCTCTTTGACAGAAAACGAGAGTTGAAATTTTGGTTTGATGGTCTAAATGCCCTCCTGCCCAAGATTATCGGGC
GAGGCCTCTACCTTTTTTCTGGACCTGTCGGGTCGGGCAAGACGACCCTCATGTATGAACTGGTCAGTCGCAAGTACCCA
GACAGTCAGATTATTACCATTGAGGATCCTGTTGAGATTAAGCAGGATAATATGCTCCAACTCCAGCTTAATCCATCTAT
CGGGATGACCTATGACCATTTGATTAAGCTCTCCCTGCGCCATCGGCCGGATGTTCTAATTATCGGAGAAATTCGCGATA
GGGAGACCGCCCAAGCAGTTATTAGGGCTAGCCTAACAGGGATCCGAGTTTTTTCGACTGTTCATGCCAAGAGTATTCCA
GGGGTCTACGCCCGGATTTTAGAGCTGGGTGTTAGCCAGGATGAGTTAGATAATTGTCTGGCCGGCATTGCCTACCAGCG
TTTAATTGGGGGAGGAGGATTGATTGATTTTGCCCAAGACCATTTTCAAAACCACCAGGCTGAGTGCTGGAATGAAAAAA
TTGACCAACTTCTTGCAGCAGGACATCTCACTGAGGAAGAGGCGGCCGCCGAAAAAATTAAAGACTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Streptococcus mutans UA159

66.026

99.681

0.658
  comGA Streptococcus mutans UA140

66.026

99.681

0.658
  comGA Streptococcus gordonii str. Challis substr. CH1

65.705

99.048

0.651
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

62.581

99.042

0.62
  comGA/cglA/cilD Streptococcus pneumoniae R6

62.581

99.042

0.62
  comGA/cglA/cilD Streptococcus pneumoniae D39

62.581

99.042

0.62
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

62.581

99.042

0.62
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

56.129

98.413

0.552
  comGA Latilactobacillus sakei subsp. sakei 23K

41.241

92.568

0.382

Multiple sequence alignment    



References

[1] J W Li et al. (2021) A Novel Competence Pathway in the Oral Pathogen Streptococcus sobrinus. Journal of Dental Research 100(5):542-548. [PMID: 33876976]