NTDB

Overview

Name	comGA/cglA/cilD	Type	Machinery gene
Locus tag	SPR_RS09430	Genome accession	NC_003098
Coordinates	1834558..1835499 (-)	Length	313 a.a.
NCBI ID	WP_000249564.1	Uniprot ID	Q8DN86
Organism	Streptococcus pneumoniae R6
Function	dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

The comGA gene is essential for the initial binding of transforming DNA to the cell surface and for the assembly of the pseudopilus, a structure required for DNA uptake.

Genomic Context

Location: 1829558..1840499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPR_RS09390 (spr1855)	comYH	1830044..1830997 (-)	954	WP_000345135.1	class I SAM-dependent methyltransferase	Machinery gene
SPR_RS09395 (spr1856)	-	1831058..1831652 (-)	595	Protein_1829	class I SAM-dependent methyltransferase	-
SPR_RS09400 (spr1858)	comGG/cglG	1831789..1832202 (-)	414	WP_000265622.1	competence type IV pilus minor pilin ComGG	Machinery gene
SPR_RS09405 (spr1859)	comGF/cglF	1832180..1832641 (-)	462	WP_000250534.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPR_RS09410	comGE/cglE	1832604..1832906 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
SPR_RS09415 (spr1861)	comGD/cglD	1832869..1833273 (-)	405	WP_000588026.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPR_RS09420 (spr1862)	comGC/cglC	1833266..1833592 (-)	327	WP_000738626.1	comG operon protein ComGC	Machinery gene
SPR_RS09425 (spr1863)	comGB/cglB	1833594..1834610 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPR_RS09430 (spr1864)	comGA/cglA/cilD	1834558..1835499 (-)	942	WP_000249564.1	competence type IV pilus ATPase ComGA	Machinery gene
SPR_RS09435 (spr1865)	-	1835575..1835940 (-)	366	WP_000286415.1	DUF1033 family protein	-
SPR_RS09440 (spr1866)	-	1836091..1837149 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
SPR_RS09445 (spr1867)	nagA	1837312..1838463 (-)	1152	WP_001134457.1	N-acetylglucosamine-6-phosphate deacetylase	-
SPR_RS09450 (spr1868)	-	1838616..1840433 (-)	1818	WP_001220853.1	acyltransferase family protein	-

Sequence

Protein

Download Length: 313 a.a. Molecular weight: 35555.47 Da Isoelectric Point: 6.1330

                                >NTDB_id=195 SPR_RS09430 WP_000249564.1 1834558..1835499(-) (comGA/cglA/cilD) [Streptococcus pneumoniae R6]

MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA

YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ

VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY

ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide

Download Length: 942 bp

                                >NTDB_id=195 SPR_RS09430 WP_000249564.1 1834558..1835499(-) (comGA/cglA/cilD) [Streptococcus pneumoniae R6]

ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT

AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG

TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC

TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT

GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT

ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA

GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC

CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG

CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT

GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG

GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC

TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8DN86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae D39	100	100	1
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	95.847	100	0.958
comYA	Streptococcus gordonii str. Challis substr. CH1	78.065	99.042	0.773
comYA	Streptococcus mutans UA159	65.916	99.361	0.655
comYA	Streptococcus mutans UA140	65.916	99.361	0.655
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	62.581	99.042	0.62
comGA	Lactococcus lactis subsp. cremoris KW2	54.808	99.681	0.546
comGA	Latilactobacillus sakei subsp. sakei 23K	41.912	91.892	0.385

Multiple sequence alignment

References

[1]	Cheng-Foh Le et al. (2016) Transcriptome analysis of Streptococcus pneumoniae treated with the designed antimicrobial peptides, DM3. Scientific Reports 6:26828. [PMID: 27225022]
[2]	Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
[3]	Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
[4]	Ping Luo et al. (2003) ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae. Molecular Microbiology 50(2):623-33. [PMID: 14617184]
[5]	Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]

Detailed information NTG0000195