Detailed information    

experimental Experimentally validated

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SPR_RS09430 Genome accession   NC_003098
Coordinates   1834558..1835499 (-) Length   313 a.a.
NCBI ID   WP_000249564.1    Uniprot ID   Q8DN86
Organism   Streptococcus pneumoniae R6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

The comGA gene is essential for the initial binding of transforming DNA to the cell surface and for the assembly of the pseudopilus, a structure required for DNA uptake.


Genomic Context

Location: 1829558..1840499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS09390 (spr1855) comYH 1830044..1830997 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  SPR_RS09395 (spr1856) - 1831058..1831652 (-) 595 Protein_1829 class I SAM-dependent methyltransferase -
  SPR_RS09400 (spr1858) comGG/cglG 1831789..1832202 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  SPR_RS09405 (spr1859) comGF/cglF 1832180..1832641 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPR_RS09410 comGE/cglE 1832604..1832906 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  SPR_RS09415 (spr1861) comGD/cglD 1832869..1833273 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  SPR_RS09420 (spr1862) comGC/cglC 1833266..1833592 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  SPR_RS09425 (spr1863) comGB/cglB 1833594..1834610 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPR_RS09430 (spr1864) comGA/cglA/cilD 1834558..1835499 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  SPR_RS09435 (spr1865) - 1835575..1835940 (-) 366 WP_000286415.1 DUF1033 family protein -
  SPR_RS09440 (spr1866) - 1836091..1837149 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  SPR_RS09445 (spr1867) nagA 1837312..1838463 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  SPR_RS09450 (spr1868) - 1838616..1840433 (-) 1818 WP_001220853.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35555.47 Da        Isoelectric Point: 6.1330

>NTDB_id=195 SPR_RS09430 WP_000249564.1 1834558..1835499(-) (comGA/cglA/cilD) [Streptococcus pneumoniae R6]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=195 SPR_RS09430 WP_000249564.1 1834558..1835499(-) (comGA/cglA/cilD) [Streptococcus pneumoniae R6]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae D39

100

100

1
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

91.892

0.385

Multiple sequence alignment    



References