Detailed information NTG0000229

Experimentally validated

Overview

• Name: comGA/cglA/cilD
• Type: Machinery gene
• Locus tag: SPD_RS09895
• Genome accession: NC_008533
• Coordinates: 1842091..1843032 (-)
• Length: 313 a.a.
• NCBI ID: WP_000249564.1
• Uniprot ID: Q8DN86
• Organism: Streptococcus pneumoniae D39
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

The comGA gene is essential for the initial binding of transforming DNA to the cell surface and for the assembly of the pseudopilus, a structure required for DNA uptake.

Genomic Context

Location: 1837091..1848032

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPD_RS09855 (SPD_1854)	comYH	1837577..1838530 (-)	954	WP_000345135.1	class I SAM-dependent methyltransferase	Machinery gene
SPD_RS09860	-	1838591..1839185 (-)	595	Protein_1835	class I SAM-dependent methyltransferase	-
SPD_RS09865 (SPD_1857)	comGG/cglG	1839322..1839735 (-)	414	WP_000265622.1	competence type IV pilus minor pilin ComGG	Machinery gene
SPD_RS09870 (SPD_1858)	comGF/cglF	1839713..1840174 (-)	462	WP_000250534.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPD_RS09875 (SPD_1859)	comGE/cglE	1840137..1840439 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
SPD_RS09880 (SPD_1860)	comGD/cglD	1840402..1840806 (-)	405	WP_000588026.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPD_RS09885 (SPD_1861)	comGC/cglC	1840799..1841125 (-)	327	WP_000738626.1	comG operon protein ComGC	Machinery gene
SPD_RS09890 (SPD_1862)	comGB/cglB	1841127..1842143 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPD_RS09895 (SPD_1863)	comGA/cglA/cilD	1842091..1843032 (-)	942	WP_000249564.1	competence type IV pilus ATPase ComGA	Machinery gene
SPD_RS09900 (SPD_1864)	-	1843108..1843473 (-)	366	WP_000286412.1	DUF1033 family protein	-
SPD_RS09905 (SPD_1865)	-	1843624..1844682 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
SPD_RS09910 (SPD_1866)	nagA	1844845..1845996 (-)	1152	WP_001134457.1	N-acetylglucosamine-6-phosphate deacetylase	-
SPD_RS09915 (SPD_1867)	-	1846149..1847966 (-)	1818	WP_001220853.1	acyltransferase family protein	-

Sequence

Protein

• Length: 313 a.a.
• Molecular weight: 35555.47 Da
• Isoelectric Point: 6.1330

>NTDB_id=229 SPD_RS09895 WP_000249564.1 1842091..1843032(-) (comGA/cglA/cilD) [Streptococcus pneumoniae D39]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide

• Length: 942 bp

>NTDB_id=229 SPD_RS09895 WP_000249564.1 1842091..1843032(-) (comGA/cglA/cilD) [Streptococcus pneumoniae D39]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-266)

3D structure

• AlphaFold DB: Q8DN86

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA/cglA/cilD	Streptococcus pneumoniae R6	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	100	100	1
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	100	100	1
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	95.847	100	0.958
comYA	Streptococcus gordonii str. Challis substr. CH1	78.065	99.042	0.773
comYA	Streptococcus mutans UA159	65.916	99.361	0.655
comYA	Streptococcus mutans UA140	65.916	99.361	0.655
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	62.581	99.042	0.62
comGA	Lactococcus lactis subsp. cremoris KW2	54.808	99.681	0.546
comGA	Latilactobacillus sakei subsp. sakei 23K	41.912	91.892	0.385

References

• [1]: Cheng-Foh Le et al. (2016) Transcriptome analysis of Streptococcus pneumoniae treated with the designed antimicrobial peptides, DM3. Scientific Reports 6:26828. [PMID: 27225022]
• [2]: Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]
• [3]: Ping Luo et al. (2003) Transient association of an alternative sigma factor, ComX, with RNA polymerase during the period of competence for genetic transformation in Streptococcus pneumoniae. Journal of Bacteriology 185(1):349-58. [PMID: 12486073]
• [4]: Ping Luo et al. (2003) ComX is a unique link between multiple quorum sensing outputs and competence in Streptococcus pneumoniae. Molecular Microbiology 50(2):623-33. [PMID: 14617184]
• [5]: Mathieu Bergé et al. (2002) Uptake of transforming DNA in Gram-positive bacteria: a view from Streptococcus pneumoniae. Molecular Microbiology 45(2):411-21. [PMID: 12123453]