Detailed information    

experimental Experimentally validated

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SM12261_RS00945 Genome accession   NZ_CP028414
Coordinates   191588..192529 (+) Length   313 a.a.
NCBI ID   WP_000249545.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 186588..197529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00925 (SM12261_0179) - 186656..188473 (+) 1818 WP_001220872.1 acyltransferase family protein -
  SM12261_RS00930 (SM12261_0180) nagA 188625..189776 (+) 1152 WP_001134449.1 N-acetylglucosamine-6-phosphate deacetylase -
  SM12261_RS00935 (SM12261_0181) - 189939..190997 (+) 1059 WP_000649460.1 zinc-dependent alcohol dehydrogenase family protein -
  SM12261_RS00940 (SM12261_0182) - 191147..191512 (+) 366 WP_000286405.1 DUF1033 family protein -
  SM12261_RS00945 (SM12261_0183) comGA/cglA/cilD 191588..192529 (+) 942 WP_000249545.1 competence type IV pilus ATPase ComGA Machinery gene
  SM12261_RS00950 (SM12261_0184) comGB/cglB 192477..193493 (+) 1017 WP_004238896.1 competence type IV pilus assembly protein ComGB Machinery gene
  SM12261_RS00955 (SM12261_0185) comGC/cglC 193495..193818 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  SM12261_RS00960 (SM12261_0186) comGD/cglD 193781..194215 (+) 435 WP_029689647.1 competence type IV pilus minor pilin ComGD Machinery gene
  SM12261_RS00965 (SM12261_0187) comGE/cglE 194178..194480 (+) 303 WP_045597458.1 competence type IV pilus minor pilin ComGE Machinery gene
  SM12261_RS00970 (SM12261_0188) comGF/cglF 194443..194904 (+) 462 WP_000250509.1 competence type IV pilus minor pilin ComGF Machinery gene
  SM12261_RS00975 (SM12261_0189) comGG/cglG 194882..195295 (+) 414 WP_000265657.1 competence type IV pilus minor pilin ComGG Machinery gene
  SM12261_RS00980 (SM12261_0190) - 195328..195915 (+) 588 WP_000679792.1 class I SAM-dependent methyltransferase -
  SM12261_RS00985 (SM12261_0191) comYH 195976..196929 (+) 954 WP_000345104.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35537.48 Da        Isoelectric Point: 6.0063

>NTDB_id=500 SM12261_RS00945 WP_000249545.1 191588..192529(+) (comGA/cglA/cilD) [Streptococcus mitis NCTC 12261]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDNMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQSTSWNAQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=500 SM12261_RS00945 WP_000249545.1 191588..192529(+) (comGA/cglA/cilD) [Streptococcus mitis NCTC 12261]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAGGATATTTATTTTGTTCCTAAGTT
AGACGCTTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAATTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTTGGAGAAAAGCGACGTAGTCAGCTTGGTTCCTGTGACTATGAA
TATGACCAGAAGATGGCGTCCTTACGGTTATCTACTGTAGGCGACTATCGAGGGCATGAGAGTTTAGTTATTCGCTTGTT
GCATGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGAAGAACTGGGCAAGCAGTACAGGCAACGGGGACTTT
ATCTTTTTGCTGGTCCAGTCGGAAGTGGCAAGACAACCTTGATGCATGAATTGGCTAAATCTCTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTAGAAATCAAGCAGGACAACATGCTCCAGTTGCAGTTGAACGAAGCAATCGGGTTGAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGAGTTTAT
GAACGTTTACTGGAGTTGGGCGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGTCAACTAGCTGGAATGCGCAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

43.019

89.527

0.385

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]