Detailed information NTG0000394

Experimentally validated

Overview

• Name: comYA
• Type: Machinery gene
• Locus tag: FSA28_RS09405
• Genome accession: NZ_CP044495
• Coordinates: 1860999..1861940 (-)
• Length: 313 a.a.
• NCBI ID: WP_002291652.1
• Uniprot ID: -
• Organism: Streptococcus mutans UA140
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

RT-PCR analysis of the orf junctions confirmed that all nine orfs of comY operon were present in a single transcript, while real-time RT-PCR analysis demonstrated that these orfs were expressed at a level very similar to that of the first orf in the operon. Mutations were constructed in all nine putative orfs. The first seven genes (comYA-G) were found to be essential for natural competence, while comYH and comYI had reduced and normal natural competence ability, respectively.

Genomic Context

Location: 1855999..1866940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FSA28_RS09365 (FSA28_1635)	comYI	1856008..1857207 (-)	1200	WP_002263443.1	acetate kinase	Machinery gene
FSA28_RS09370 (FSA28_1636)	comYH	1857266..1858219 (-)	954	WP_002291646.1	class I SAM-dependent methyltransferase	Machinery gene
FSA28_RS09375 (FSA28_1637)	comYG	1858274..1858663 (-)	390	WP_002263441.1	competence type IV pilus minor pilin ComGG	Machinery gene
FSA28_RS09380 (FSA28_1638)	comYF	1858641..1859075 (-)	435	WP_002264245.1	competence type IV pilus minor pilin ComGF	Machinery gene
FSA28_RS09385 (FSA28_1639)	comYE	1859062..1859355 (-)	294	WP_002263439.1	competence type IV pilus minor pilin ComGE	Machinery gene
FSA28_RS09390 (FSA28_1640)	comYD	1859327..1859731 (-)	405	WP_002263438.1	competence type IV pilus minor pilin ComGD	Machinery gene
FSA28_RS09395 (FSA28_1641)	comYC	1859718..1860032 (-)	315	WP_002263437.1	competence type IV pilus major pilin ComGC	Machinery gene
FSA28_RS09400 (FSA28_1642)	comYB	1860032..1861063 (-)	1032	WP_257638827.1	competence type IV pilus assembly protein ComGB	Machinery gene
FSA28_RS09405 (FSA28_1643)	comYA	1860999..1861940 (-)	942	WP_002291652.1	competence type IV pilus ATPase ComGA	Machinery gene
FSA28_RS09410 (FSA28_1644)	-	1862071..1862454 (-)	384	WP_002263434.1	DUF1033 family protein	-
FSA28_RS09415 (FSA28_1645)	rpoC	1862582..1866211 (-)	3630	WP_002263433.1	DNA-directed RNA polymerase subunit beta'	-

Sequence

Protein

• Length: 313 a.a.
• Molecular weight: 35686.05 Da
• Isoelectric Point: 7.4663

>NTDB_id=394 FSA28_RS09405 WP_002291652.1 1860999..1861940(-) (comYA) [Streptococcus mutans UA140]
MVQKLGRKIIAEAVQNKAQDIYIIPKKESYELYMRIGNERRFIDVYDHHFMANLISHFKFVAGMNVGEKRRSQLGSCDYS
YDEGKVISLRLSSVGDYRGYESLVIRLLFSGRHDLKFWFNSMDLILNEIKGRGLYLFSGPVGSGKTSLMYQLVKLRFETK
QVITIEDPVEIKQDNMLQLQLNESIDMTYDNLIKLSLRHRPDILIIGEIRDKETARAVIRASLTGAMVFSTIHAKSIPGV
YARMLELGTSSEELQNALCGIVYQRLIGGGGIIDFAKGDYQNHSAKKWNKQIYDLFTAGYITEGEAQAEEIED

Nucleotide

• Length: 942 bp

>NTDB_id=394 FSA28_RS09405 WP_002291652.1 1860999..1861940(-) (comYA) [Streptococcus mutans UA140]
ATGGTTCAAAAATTAGGTAGAAAAATCATTGCCGAAGCTGTTCAAAATAAAGCACAAGATATCTATATTATTCCTAAAAA
AGAATCTTATGAGCTTTATATGAGAATAGGGAATGAAAGGAGGTTCATTGATGTTTATGACCATCATTTCATGGCTAATC
TTATTAGTCACTTTAAATTTGTTGCAGGAATGAATGTTGGTGAGAAAAGACGAAGTCAGCTGGGCTCTTGTGATTATTCT
TATGATGAAGGGAAAGTTATTTCATTAAGGTTATCAAGTGTAGGAGATTATCGTGGTTATGAAAGTTTGGTTATTCGCTT
ACTTTTTTCTGGACGTCACGATTTAAAATTCTGGTTTAACAGTATGGATTTGATTTTAAATGAAATCAAGGGGCGAGGGC
TTTATCTTTTTTCAGGTCCAGTTGGAAGTGGCAAAACAAGTTTGATGTATCAATTGGTAAAACTAAGATTTGAGACCAAA
CAGGTAATTACCATTGAAGATCCTGTGGAAATTAAGCAAGATAATATGTTACAACTGCAGCTTAATGAAAGCATTGATAT
GACTTATGATAATTTAATTAAATTATCTCTGAGGCATCGTCCGGATATTTTAATTATTGGAGAAATTCGAGATAAAGAAA
CCGCCAGAGCTGTTATTAGAGCAAGTCTTACAGGGGCAATGGTATTCTCAACTATCCATGCTAAAAGCATTCCAGGTGTT
TATGCTAGGATGTTAGAATTGGGTACTAGTTCAGAAGAATTACAAAATGCTTTGTGTGGTATTGTTTATCAACGTTTAAT
AGGGGGAGGAGGGATTATTGACTTTGCCAAAGGGGATTATCAAAACCACTCAGCAAAAAAGTGGAACAAACAAATTTATG
ACCTTTTTACAGCAGGATATATCACTGAAGGAGAAGCACAAGCAGAAGAAATTGAAGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (8-267)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYA	Streptococcus mutans UA159	99.681	100	0.997
comYA	Streptococcus gordonii str. Challis substr. CH1	67.093	100	0.671
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	66.026	99.681	0.658
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	65.916	99.361	0.655
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	65.916	99.361	0.655
comGA/cglA/cilD	Streptococcus pneumoniae R6	65.916	99.361	0.655
comGA/cglA/cilD	Streptococcus pneumoniae D39	65.916	99.361	0.655
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	65.916	99.361	0.655
comGA	Lactococcus lactis subsp. cremoris KW2	52.412	99.361	0.521
comGA	Latilactobacillus sakei subsp. sakei 23K	38.909	92.905	0.361

References

• [1]: Justin Merritt et al. (2005) A unique nine-gene comY operon in Streptococcus mutans. Microbiology (Reading, England) 151(Pt 1):157-166. [PMID: 15632435]