Detailed information    

experimental Experimentally validated

Overview

Name   comYI   Type   Machinery gene
Locus tag   FSA28_RS09365 Genome accession   NZ_CP044495
Coordinates   1856008..1857207 (-) Length   399 a.a.
NCBI ID   WP_002263443.1    Uniprot ID   A0AAX1K1X0
Organism   Streptococcus mutans UA140     
Function   not require for competence   
Unclear

Function

RT-PCR analysis of the orf junctions confirmed that all nine orfs of comY operon were present in a single transcript, while real-time RT-PCR analysis demonstrated that these orfs were expressed at a level very similar to that of the first orf in the operon. Mutations were constructed in all nine putative orfs. The first seven genes (comYA-G) were found to be essential for natural competence, while comYH and comYI had reduced and normal natural competence ability, respectively.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1852966..1855621 1856008..1857207 flank 387


Gene organization within MGE regions


Location: 1852966..1857207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSA28_RS09340 (FSA28_1631) - 1852966..1854071 (+) 1106 WP_088741907.1 IS3 family transposase -
  FSA28_RS09345 (FSA28_1632) - 1854114..1854497 (-) 384 WP_088741902.1 hypothetical protein -
  FSA28_RS09350 (FSA28_1633) - 1854466..1854711 (-) 246 WP_002265894.1 helix-turn-helix transcriptional regulator -
  FSA28_RS09360 (FSA28_1634) tnpA 1855331..1855794 (-) 464 Protein_1743 IS200/IS605 family transposase -
  FSA28_RS09365 (FSA28_1635) comYI 1856008..1857207 (-) 1200 WP_002263443.1 acetate kinase Machinery gene

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 43732.20 Da        Isoelectric Point: 6.0985

>NTDB_id=402 FSA28_RS09365 WP_002263443.1 1856008..1857207(-) (comYI) [Streptococcus mutans UA140]
MSKTISINAGSSSLKWQLYLMPEEKVLAKGLIERIGKDDAISTVKFNGQEASETLAIKDHTAAVKILLDDLIHLDIIKSY
DEITGVGHRVVAGGTYFNESALVDSEEVIKKVEELALLAPLHNKANAAGIRAFKKILPDITSVVVFDTAFHTTMPEVAYR
YPLPNKYFTENQVRKYGAHGTSHYYVAHEAAKILEKPIEKLKLITVHMGNGVSLTAVDGGKSVDTSMGFTPLGGVMMGTR
TGDLDPAVIPYLMDNTEDFKTPEDIRRIFNNESGLLGISELSNDMREIEAATAAGNKNATLAYNMFIDRIIKHIGAYAAV
MNGVDAIVFTAGIGENDAHIRSEIMKHFDWLGADIVTEKNEKRPVYGVISSNAAKVKVLVIPTDEELVIARDVERLKTK

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=402 FSA28_RS09365 WP_002263443.1 1856008..1857207(-) (comYI) [Streptococcus mutans UA140]
ATGTCAAAAACTATTTCAATTAATGCAGGTTCTTCTAGCCTTAAATGGCAGCTATACTTGATGCCTGAAGAAAAAGTACT
TGCAAAGGGGTTAATTGAGCGTATTGGCAAGGATGATGCTATTTCTACTGTAAAATTTAATGGTCAAGAAGCTTCAGAAA
CCTTAGCTATTAAAGACCATACTGCAGCAGTGAAAATTCTCTTAGATGATTTAATCCATTTGGATATTATTAAATCGTAT
GATGAAATCACAGGTGTAGGGCACCGTGTAGTTGCTGGTGGAACTTACTTTAATGAATCCGCTTTAGTTGATAGTGAAGA
AGTCATTAAAAAGGTTGAAGAGTTAGCACTCTTAGCGCCCTTGCATAATAAAGCAAATGCTGCAGGAATTCGAGCATTTA
AGAAAATTCTACCAGATATTACCAGTGTCGTTGTCTTTGATACTGCTTTCCACACTACGATGCCGGAGGTTGCCTACCGC
TATCCATTGCCGAATAAGTATTTTACAGAAAATCAGGTTCGTAAATATGGAGCTCATGGAACTAGCCATTATTATGTTGC
TCATGAAGCAGCCAAAATATTAGAAAAACCGATTGAAAAACTTAAATTGATTACGGTTCATATGGGAAACGGGGTTTCTC
TTACGGCAGTTGATGGCGGAAAGTCAGTTGACACGTCAATGGGCTTTACACCACTGGGTGGTGTGATGATGGGAACACGA
ACGGGAGATCTTGATCCAGCCGTTATTCCTTATTTAATGGATAATACAGAAGATTTTAAAACACCAGAAGATATTCGCCG
TATTTTCAATAATGAGTCAGGTTTGCTGGGAATTTCTGAGTTATCAAATGATATGCGTGAAATTGAGGCGGCTACAGCAG
CTGGTAATAAGAATGCTACCTTGGCTTATAACATGTTCATTGACCGTATTATTAAGCATATTGGTGCTTACGCTGCTGTG
ATGAATGGAGTTGATGCTATTGTCTTTACGGCGGGTATTGGTGAAAATGATGCACATATTCGAAGCGAAATTATGAAACA
CTTTGATTGGCTTGGAGCTGATATCGTTACCGAAAAGAACGAAAAGCGTCCAGTTTACGGGGTCATATCATCGAATGCCG
CTAAAGTTAAAGTTTTGGTGATTCCCACTGATGAAGAGCTTGTTATTGCACGTGATGTCGAGCGTTTGAAAACTAAATAA

Domains

Predicted by InterproScan.

Acetokinase family (Acetate_kinase)

(4-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYI Streptococcus mutans UA159

100

100

1

Multiple sequence alignment    



References

[1] Justin Merritt et al. (2005) A unique nine-gene comY operon in Streptococcus mutans. Microbiology (Reading, England) 151(Pt 1):157-166. [PMID: 15632435]