Detailed information    

experimental Experimentally validated

Overview

Name   comYI   Type   Machinery gene
Locus tag   FSA28_RS09365 Genome accession   NZ_CP044495
Coordinates   1856008..1857207 (-) Length   399 a.a.
NCBI ID   WP_002263443.1    Uniprot ID   A0AAX1K1X0
Organism   Streptococcus mutans UA140     
Function   not require for competence   
Unclear

Function

RT-PCR analysis of the orf junctions confirmed that all nine orfs of comY operon were present in a single transcript, while real-time RT-PCR analysis demonstrated that these orfs were expressed at a level very similar to that of the first orf in the operon. Mutations were constructed in all nine putative orfs. The first seven genes (comYA-G) were found to be essential for natural competence, while comYH and comYI had reduced and normal natural competence ability, respectively.


Genomic Context

Location: 1851008..1862207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSA28_RS09330 (FSA28_1628) proC 1851264..1852034 (+) 771 WP_002291585.1 pyrroline-5-carboxylate reductase -
  FSA28_RS09335 (FSA28_1629) - 1852119..1852781 (-) 663 WP_002266816.1 CPBP family intramembrane glutamic endopeptidase -
  FSA28_RS09340 (FSA28_1631) - 1852966..1854071 (+) 1106 WP_088741907.1 IS3 family transposase -
  FSA28_RS09345 (FSA28_1632) - 1854114..1854497 (-) 384 WP_088741902.1 hypothetical protein -
  FSA28_RS09350 (FSA28_1633) - 1854466..1854711 (-) 246 WP_002265894.1 helix-turn-helix transcriptional regulator -
  FSA28_RS09360 (FSA28_1634) tnpA 1855331..1855794 (-) 464 Protein_1743 IS200/IS605 family transposase -
  FSA28_RS09365 (FSA28_1635) comYI 1856008..1857207 (-) 1200 WP_002263443.1 acetate kinase Machinery gene
  FSA28_RS09370 (FSA28_1636) comYH 1857266..1858219 (-) 954 WP_002291646.1 class I SAM-dependent methyltransferase Machinery gene
  FSA28_RS09375 (FSA28_1637) comYG 1858274..1858663 (-) 390 WP_002263441.1 competence type IV pilus minor pilin ComGG Machinery gene
  FSA28_RS09380 (FSA28_1638) comYF 1858641..1859075 (-) 435 WP_002264245.1 competence type IV pilus minor pilin ComGF Machinery gene
  FSA28_RS09385 (FSA28_1639) comYE 1859062..1859355 (-) 294 WP_002263439.1 competence type IV pilus minor pilin ComGE Machinery gene
  FSA28_RS09390 (FSA28_1640) comYD 1859327..1859731 (-) 405 WP_002263438.1 competence type IV pilus minor pilin ComGD Machinery gene
  FSA28_RS09395 (FSA28_1641) comYC 1859718..1860032 (-) 315 WP_002263437.1 competence type IV pilus major pilin ComGC Machinery gene
  FSA28_RS09400 (FSA28_1642) comYB 1860032..1861063 (-) 1032 WP_257638827.1 competence type IV pilus assembly protein ComGB Machinery gene
  FSA28_RS09405 (FSA28_1643) comYA 1860999..1861940 (-) 942 WP_002291652.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 43732.20 Da        Isoelectric Point: 6.0985

>NTDB_id=402 FSA28_RS09365 WP_002263443.1 1856008..1857207(-) (comYI) [Streptococcus mutans UA140]
MSKTISINAGSSSLKWQLYLMPEEKVLAKGLIERIGKDDAISTVKFNGQEASETLAIKDHTAAVKILLDDLIHLDIIKSY
DEITGVGHRVVAGGTYFNESALVDSEEVIKKVEELALLAPLHNKANAAGIRAFKKILPDITSVVVFDTAFHTTMPEVAYR
YPLPNKYFTENQVRKYGAHGTSHYYVAHEAAKILEKPIEKLKLITVHMGNGVSLTAVDGGKSVDTSMGFTPLGGVMMGTR
TGDLDPAVIPYLMDNTEDFKTPEDIRRIFNNESGLLGISELSNDMREIEAATAAGNKNATLAYNMFIDRIIKHIGAYAAV
MNGVDAIVFTAGIGENDAHIRSEIMKHFDWLGADIVTEKNEKRPVYGVISSNAAKVKVLVIPTDEELVIARDVERLKTK

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=402 FSA28_RS09365 WP_002263443.1 1856008..1857207(-) (comYI) [Streptococcus mutans UA140]
ATGTCAAAAACTATTTCAATTAATGCAGGTTCTTCTAGCCTTAAATGGCAGCTATACTTGATGCCTGAAGAAAAAGTACT
TGCAAAGGGGTTAATTGAGCGTATTGGCAAGGATGATGCTATTTCTACTGTAAAATTTAATGGTCAAGAAGCTTCAGAAA
CCTTAGCTATTAAAGACCATACTGCAGCAGTGAAAATTCTCTTAGATGATTTAATCCATTTGGATATTATTAAATCGTAT
GATGAAATCACAGGTGTAGGGCACCGTGTAGTTGCTGGTGGAACTTACTTTAATGAATCCGCTTTAGTTGATAGTGAAGA
AGTCATTAAAAAGGTTGAAGAGTTAGCACTCTTAGCGCCCTTGCATAATAAAGCAAATGCTGCAGGAATTCGAGCATTTA
AGAAAATTCTACCAGATATTACCAGTGTCGTTGTCTTTGATACTGCTTTCCACACTACGATGCCGGAGGTTGCCTACCGC
TATCCATTGCCGAATAAGTATTTTACAGAAAATCAGGTTCGTAAATATGGAGCTCATGGAACTAGCCATTATTATGTTGC
TCATGAAGCAGCCAAAATATTAGAAAAACCGATTGAAAAACTTAAATTGATTACGGTTCATATGGGAAACGGGGTTTCTC
TTACGGCAGTTGATGGCGGAAAGTCAGTTGACACGTCAATGGGCTTTACACCACTGGGTGGTGTGATGATGGGAACACGA
ACGGGAGATCTTGATCCAGCCGTTATTCCTTATTTAATGGATAATACAGAAGATTTTAAAACACCAGAAGATATTCGCCG
TATTTTCAATAATGAGTCAGGTTTGCTGGGAATTTCTGAGTTATCAAATGATATGCGTGAAATTGAGGCGGCTACAGCAG
CTGGTAATAAGAATGCTACCTTGGCTTATAACATGTTCATTGACCGTATTATTAAGCATATTGGTGCTTACGCTGCTGTG
ATGAATGGAGTTGATGCTATTGTCTTTACGGCGGGTATTGGTGAAAATGATGCACATATTCGAAGCGAAATTATGAAACA
CTTTGATTGGCTTGGAGCTGATATCGTTACCGAAAAGAACGAAAAGCGTCCAGTTTACGGGGTCATATCATCGAATGCCG
CTAAAGTTAAAGTTTTGGTGATTCCCACTGATGAAGAGCTTGTTATTGCACGTGATGTCGAGCGTTTGAAAACTAAATAA

Domains

Predicted by InterProScan.

Acetokinase family (Acetate_kinase)

(4-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYI Streptococcus mutans UA159

100

100

1

Multiple sequence alignment    



References

[1] Justin Merritt et al. (2005) A unique nine-gene comY operon in Streptococcus mutans. Microbiology (Reading, England) 151(Pt 1):157-166. [PMID: 15632435]