Detailed information    

experimental Experimentally validated

Overview

Name   comYC   Type   Machinery gene
Locus tag   FSA28_RS09395 Genome accession   NZ_CP044495
Coordinates   1859718..1860032 (-) Length   104 a.a.
NCBI ID   WP_002263437.1    Uniprot ID   -
Organism   Streptococcus mutans UA140     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

RT-PCR analysis of the orf junctions confirmed that all nine orfs of comY operon were present in a single transcript, while real-time RT-PCR analysis demonstrated that these orfs were expressed at a level very similar to that of the first orf in the operon. Mutations were constructed in all nine putative orfs. The first seven genes (comYA-G) were found to be essential for natural competence, while comYH and comYI had reduced and normal natural competence ability, respectively.


Genomic Context

Location: 1854718..1865032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSA28_RS09360 (FSA28_1634) tnpA 1855331..1855794 (-) 464 Protein_1743 IS200/IS605 family transposase -
  FSA28_RS09365 (FSA28_1635) comYI 1856008..1857207 (-) 1200 WP_002263443.1 acetate kinase Machinery gene
  FSA28_RS09370 (FSA28_1636) comYH 1857266..1858219 (-) 954 WP_002291646.1 class I SAM-dependent methyltransferase Machinery gene
  FSA28_RS09375 (FSA28_1637) comYG 1858274..1858663 (-) 390 WP_002263441.1 competence type IV pilus minor pilin ComGG Machinery gene
  FSA28_RS09380 (FSA28_1638) comYF 1858641..1859075 (-) 435 WP_002264245.1 competence type IV pilus minor pilin ComGF Machinery gene
  FSA28_RS09385 (FSA28_1639) comYE 1859062..1859355 (-) 294 WP_002263439.1 competence type IV pilus minor pilin ComGE Machinery gene
  FSA28_RS09390 (FSA28_1640) comYD 1859327..1859731 (-) 405 WP_002263438.1 competence type IV pilus minor pilin ComGD Machinery gene
  FSA28_RS09395 (FSA28_1641) comYC 1859718..1860032 (-) 315 WP_002263437.1 competence type IV pilus major pilin ComGC Machinery gene
  FSA28_RS09400 (FSA28_1642) comYB 1860032..1861063 (-) 1032 WP_257638827.1 competence type IV pilus assembly protein ComGB Machinery gene
  FSA28_RS09405 (FSA28_1643) comYA 1860999..1861940 (-) 942 WP_002291652.1 competence type IV pilus ATPase ComGA Machinery gene
  FSA28_RS09410 (FSA28_1644) - 1862071..1862454 (-) 384 WP_002263434.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 104 a.a.        Molecular weight: 11591.60 Da        Isoelectric Point: 10.2206

>NTDB_id=396 FSA28_RS09395 WP_002263437.1 1859718..1860032(-) (comYC) [Streptococcus mutans UA140]
MKKIKRVSVRAFTLIEMLVVLLIISILLLLFVPNLTKQKDKVKDTGGSAVVKVVESQAELYELNKTDSATLSKLVASGDI
TQEQANSYKDYYAKHPSKTRHVAN

Nucleotide


Download         Length: 315 bp        

>NTDB_id=396 FSA28_RS09395 WP_002263437.1 1859718..1860032(-) (comYC) [Streptococcus mutans UA140]
ATGAAAAAGATCAAGAGAGTCAGTGTACGGGCTTTTACGCTTATAGAAATGTTAGTAGTTCTTCTCATTATTAGTATTCT
TTTACTATTATTTGTTCCTAATTTAACTAAGCAAAAAGATAAGGTAAAAGATACTGGCGGTTCTGCCGTGGTTAAAGTTG
TAGAAAGTCAGGCTGAGCTTTATGAGCTTAATAAAACCGATTCTGCTACTCTTTCTAAATTAGTAGCTAGTGGTGATATT
ACACAAGAACAGGCAAATTCATACAAGGATTACTATGCAAAACACCCAAGTAAAACACGGCATGTCGCGAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYC Streptococcus mutans UA159

100

100

1
  comYC Streptococcus gordonii str. Challis substr. CH1

71.429

100

0.721
  comGC/cglC Streptococcus mitis SK321

66.667

95.192

0.635
  comGC/cglC Streptococcus mitis NCTC 12261

69.565

88.462

0.615
  comGC/cglC Streptococcus pneumoniae R6

63.636

95.192

0.606
  comGC/cglC Streptococcus pneumoniae TIGR4

63.636

95.192

0.606
  comGC/cglC Streptococcus pneumoniae D39

63.636

95.192

0.606
  comGC/cglC Streptococcus pneumoniae Rx1

63.636

95.192

0.606
  comYC Streptococcus suis isolate S10

60.227

94.624

0.57
  comGC Lactococcus lactis subsp. cremoris KW2

55.446

97.115

0.538

Multiple sequence alignment    



References

[1] Justin Merritt et al. (2005) A unique nine-gene comY operon in Streptococcus mutans. Microbiology (Reading, England) 151(Pt 1):157-166. [PMID: 15632435]