Detailed information    

experimental Experimentally validated

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   SM12261_RS00955 Genome accession   NZ_CP028414
Coordinates   193495..193818 (+) Length   107 a.a.
NCBI ID   WP_000738641.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 188495..198818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00930 (SM12261_0180) nagA 188625..189776 (+) 1152 WP_001134449.1 N-acetylglucosamine-6-phosphate deacetylase -
  SM12261_RS00935 (SM12261_0181) - 189939..190997 (+) 1059 WP_000649460.1 zinc-dependent alcohol dehydrogenase family protein -
  SM12261_RS00940 (SM12261_0182) - 191147..191512 (+) 366 WP_000286405.1 DUF1033 family protein -
  SM12261_RS00945 (SM12261_0183) comGA/cglA/cilD 191588..192529 (+) 942 WP_000249545.1 competence type IV pilus ATPase ComGA Machinery gene
  SM12261_RS00950 (SM12261_0184) comGB/cglB 192477..193493 (+) 1017 WP_004238896.1 competence type IV pilus assembly protein ComGB Machinery gene
  SM12261_RS00955 (SM12261_0185) comGC/cglC 193495..193818 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  SM12261_RS00960 (SM12261_0186) comGD/cglD 193781..194215 (+) 435 WP_029689647.1 competence type IV pilus minor pilin ComGD Machinery gene
  SM12261_RS00965 (SM12261_0187) comGE/cglE 194178..194480 (+) 303 WP_045597458.1 competence type IV pilus minor pilin ComGE Machinery gene
  SM12261_RS00970 (SM12261_0188) comGF/cglF 194443..194904 (+) 462 WP_000250509.1 competence type IV pilus minor pilin ComGF Machinery gene
  SM12261_RS00975 (SM12261_0189) comGG/cglG 194882..195295 (+) 414 WP_000265657.1 competence type IV pilus minor pilin ComGG Machinery gene
  SM12261_RS00980 (SM12261_0190) - 195328..195915 (+) 588 WP_000679792.1 class I SAM-dependent methyltransferase -
  SM12261_RS00985 (SM12261_0191) comYH 195976..196929 (+) 954 WP_000345104.1 class I SAM-dependent methyltransferase Machinery gene
  SM12261_RS00990 (SM12261_0192) - 196979..198169 (+) 1191 WP_000167778.1 acetate kinase -
  SM12261_RS00995 (SM12261_0193) rnpA 198319..198690 (+) 372 WP_000739243.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 107 a.a.        Molecular weight: 11958.09 Da        Isoelectric Point: 9.9097

>NTDB_id=502 SM12261_RS00955 WP_000738641.1 193495..193818(+) (comGC/cglC) [Streptococcus mitis NCTC 12261]
MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLLLLFVPNLTKQKEAVNDKGKAAVVKVVESQAELYSLDKNEDASLSKLQA
DGRITEEQAKAYKEYHAKQNTSQTVAD

Nucleotide


Download         Length: 324 bp        

>NTDB_id=502 SM12261_RS00955 WP_000738641.1 193495..193818(+) (comGC/cglC) [Streptococcus mitis NCTC 12261]
ATGAAAAAAATGATGACATTCTTGAAAAAAGCGAAAGTGAAAGCTTTCACGCTTGTGGAAATGTTGGTGGTCTTGCTCAT
CATCAGCGTGCTTCTCTTGCTCTTTGTACCTAATCTGACCAAGCAAAAAGAAGCGGTCAATGACAAAGGAAAAGCTGCTG
TTGTTAAGGTGGTGGAAAGTCAGGCAGAGCTTTATAGCTTGGATAAAAATGAAGATGCTAGTCTAAGCAAGTTACAAGCA
GATGGGCGAATCACAGAAGAACAGGCTAAAGCTTATAAAGAATACCATGCTAAACAAAATACCAGTCAAACCGTTGCAGA
TTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus mitis SK321

93.204

96.262

0.897
  comGC/cglC Streptococcus pneumoniae Rx1

92.157

95.327

0.879
  comGC/cglC Streptococcus pneumoniae D39

92.157

95.327

0.879
  comGC/cglC Streptococcus pneumoniae R6

92.157

95.327

0.879
  comGC/cglC Streptococcus pneumoniae TIGR4

90.291

96.262

0.869
  comYC Streptococcus gordonii str. Challis substr. CH1

69.524

100

0.695
  comYC Streptococcus suis isolate S10

68.235

91.398

0.624
  comYC Streptococcus mutans UA140

69.565

88.462

0.615
  comYC Streptococcus mutans UA159

69.565

88.462

0.615
  comGC Lactococcus lactis subsp. cremoris KW2

54.455

94.393

0.514
  comGC Staphylococcus aureus MW2

51.163

83.495

0.427
  comGC Staphylococcus aureus N315

51.163

83.495

0.427
  comGC Latilactobacillus sakei subsp. sakei 23K

44.186

86.869

0.384

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]