Detailed information NTG0000503

Experimentally validated

Overview

• Name: comGD/cglD
• Type: Machinery gene
• Locus tag: SM12261_RS00960
• Genome accession: NZ_CP028414
• Coordinates: 193781..194215 (+)
• Length: 144 a.a.
• NCBI ID: WP_029689647.1
• Uniprot ID: -
• Organism: Streptococcus mitis NCTC 12261
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 188781..199215

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM12261_RS00935 (SM12261_0181)	-	189939..190997 (+)	1059	WP_000649460.1	zinc-dependent alcohol dehydrogenase family protein	-
SM12261_RS00940 (SM12261_0182)	-	191147..191512 (+)	366	WP_000286405.1	DUF1033 family protein	-
SM12261_RS00945 (SM12261_0183)	comGA/cglA/cilD	191588..192529 (+)	942	WP_000249545.1	competence type IV pilus ATPase ComGA	Machinery gene
SM12261_RS00950 (SM12261_0184)	comGB/cglB	192477..193493 (+)	1017	WP_004238896.1	competence type IV pilus assembly protein ComGB	Machinery gene
SM12261_RS00955 (SM12261_0185)	comGC/cglC	193495..193818 (+)	324	WP_000738641.1	comG operon protein ComGC	Machinery gene
SM12261_RS00960 (SM12261_0186)	comGD/cglD	193781..194215 (+)	435	WP_029689647.1	competence type IV pilus minor pilin ComGD	Machinery gene
SM12261_RS00965 (SM12261_0187)	comGE/cglE	194178..194480 (+)	303	WP_045597458.1	competence type IV pilus minor pilin ComGE	Machinery gene
SM12261_RS00970 (SM12261_0188)	comGF/cglF	194443..194904 (+)	462	WP_000250509.1	competence type IV pilus minor pilin ComGF	Machinery gene
SM12261_RS00975 (SM12261_0189)	comGG/cglG	194882..195295 (+)	414	WP_000265657.1	competence type IV pilus minor pilin ComGG	Machinery gene
SM12261_RS00980 (SM12261_0190)	-	195328..195915 (+)	588	WP_000679792.1	class I SAM-dependent methyltransferase	-
SM12261_RS00985 (SM12261_0191)	comYH	195976..196929 (+)	954	WP_000345104.1	class I SAM-dependent methyltransferase	Machinery gene
SM12261_RS00990 (SM12261_0192)	-	196979..198169 (+)	1191	WP_000167778.1	acetate kinase	-
SM12261_RS00995 (SM12261_0193)	rnpA	198319..198690 (+)	372	WP_000739243.1	ribonuclease P protein component	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 15771.28 Da
• Isoelectric Point: 10.3971

>NTDB_id=503 SM12261_RS00960 WP_029689647.1 193781..194215(+) (comGD/cglD) [Streptococcus mitis NCTC 12261]
MLNKIPVKPLQIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEFEELYRETQKRSVASQQKTSLNLDGKT
ISNGSQKLTVPKGIQAPSGQSITFDRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKD

Nucleotide

• Length: 435 bp

>NTDB_id=503 SM12261_RS00960 WP_029689647.1 193781..194215(+) (comGD/cglD) [Streptococcus mitis NCTC 12261]
ATGCTAAACAAAATACCAGTCAAACCGTTGCAGATTAAGGCCTTTACCATGCTTGAAAGTCTCTTGGTTTTGGGACTTGT
GAGTATCCTTGCCTTGGGCTTGTCTGGCTCTGTCCAGTCCACTTTTGCGGCGGTAGAGGAGCAGATTTTCTTTATGGAGT
TTGAGGAACTTTATCGGGAAACCCAAAAGCGCAGTGTAGCCAGTCAGCAAAAGACTAGTCTAAACTTAGATGGGAAGACA
ATCAGTAATGGCAGTCAAAAGTTGACAGTTCCTAAAGGAATTCAGGCACCATCAGGCCAAAGTATTACATTTGACCGAGC
TGGGGGCAATTCGTCCCTAGCTAAGGTTGAATTTCAGACCAGTAAAGGAGCGATTCGTTATCAATTATATCTAGGAAATG
GAAAAATTAAACGCATTAAGGAAACAAAAGATTAG

Domains

No domain identified.

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGD/cglD	Streptococcus pneumoniae D39	96.992	99.254	0.963
comGD/cglD	Streptococcus pneumoniae R6	96.992	99.254	0.963
comGD/cglD	Streptococcus pneumoniae Rx1	96.992	99.254	0.963
comGD/cglD	Streptococcus mitis SK321	96.241	99.254	0.955
comGD/cglD	Streptococcus pneumoniae TIGR4	96.241	99.254	0.955
comYD	Streptococcus gordonii str. Challis substr. CH1	55.97	94.366	0.528
comYD	Streptococcus mutans UA159	49.612	96.269	0.478
comYD	Streptococcus mutans UA140	49.612	96.269	0.478

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]