Detailed information    

experimental Experimentally validated

Overview

Name   comGA   Type   Machinery gene
Locus tag   KW2_RS10855 Genome accession   NC_022369
Coordinates   2244868..2245848 (-) Length   326 a.a.
NCBI ID   WP_011836048.1    Uniprot ID   A2RNS9
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus   
DNA binding and uptake

Function

The competence sigma factor comX is functional for the activation of the comG operon from strain KW2 when it is constitutively produced.


Genomic Context

Location: 2239868..2250848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS10810 (kw2_2176) - 2239884..2240621 (-) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  KW2_RS10815 (kw2_2177) - 2240800..2241642 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  KW2_RS10820 (kw2_2178) - 2241639..2242076 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  KW2_RS10825 (kw2_2179) comGG 2242157..2242456 (-) 300 WP_021037947.1 competence type IV pilus minor pilin ComGG Machinery gene
  KW2_RS10830 (kw2_2180) comGF 2242480..2242905 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  KW2_RS10835 (kw2_2181) comGE 2242889..2243185 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  KW2_RS10840 (kw2_2182) comGD 2243157..2243600 (-) 444 WP_096816401.1 competence type IV pilus minor pilin ComGD Machinery gene
  KW2_RS10845 (kw2_2183) comGC 2243548..2243898 (-) 351 WP_051303741.1 competence type IV pilus major pilin ComGC Machinery gene
  KW2_RS10850 (kw2_2184) comGB 2243943..2244968 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  KW2_RS10855 (kw2_2185) comGA 2244868..2245848 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  KW2_RS10860 (kw2_2186) - 2245962..2249744 (-) 3783 Protein_2127 PolC-type DNA polymerase III -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comGA positive effect
  comX comGG positive effect
  comX comEA positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36975.27 Da        Isoelectric Point: 5.9451

>NTDB_id=586 KW2_RS10855 WP_011836048.1 2244868..2245848(-) (comGA) [Lactococcus lactis subsp. cremoris KW2]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEEVNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFAGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIDSDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=586 KW2_RS10855 WP_011836048.1 2244868..2245848(-) (comGA) [Lactococcus lactis subsp. cremoris KW2]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAAGTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAACGAATCATTGGTTATCCGTCT
TCTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGATGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTGCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGATTCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATTCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTAAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2RNS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.129

98.413

0.552
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.808

99.681

0.546
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.808

99.681

0.546
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.808

99.681

0.546
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.808

99.681

0.546
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.808

99.681

0.546
  comYA Streptococcus gordonii str. Challis substr. CH1

54.114

99.06

0.536
  comYA Streptococcus mutans UA159

52.412

99.361

0.521
  comYA Streptococcus mutans UA140

52.412

99.361

0.521
  comGA Latilactobacillus sakei subsp. sakei 23K

37.793

100

0.382

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]