Detailed information    

experimental Experimentally validated

Overview

Name   dprA   Type   Machinery gene
Locus tag   KW2_RS05940 Genome accession   NC_022369
Coordinates   1241245..1242093 (+) Length   282 a.a.
NCBI ID   WP_021037268.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   ssDNA binding; loading RecA onto ssDNA   
DNA processing

Function

ComX induce the expression of late competence genes.


Genomic Context

Location: 1236245..1247093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS05915 (kw2_1187) ilvN 1236978..1237454 (+) 477 WP_021037263.1 acetolactate synthase small subunit -
  KW2_RS05920 (kw2_1188) ilvC 1237500..1238522 (+) 1023 WP_021037264.1 ketol-acid reductoisomerase -
  KW2_RS05925 (kw2_1189) ilvA 1238581..1239831 (+) 1251 WP_021037265.1 threonine ammonia-lyase IlvA -
  KW2_RS05930 (kw2_1190) budA 1239967..1240677 (+) 711 WP_021037266.1 acetolactate decarboxylase -
  KW2_RS05935 (kw2_1191) - 1240717..1241097 (+) 381 WP_021037267.1 RidA family protein -
  KW2_RS05940 (kw2_1192) dprA 1241245..1242093 (+) 849 WP_021037268.1 DNA-processing protein DprA Machinery gene
  KW2_RS05945 (kw2_1193) topA 1242359..1244479 (+) 2121 WP_021037269.1 type I DNA topoisomerase -
  KW2_RS05950 (kw2_1194) trmFO 1244551..1245897 (+) 1347 WP_021037270.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  KW2_RS05955 (kw2_1195) xerS 1245948..1247018 (-) 1071 WP_011835149.1 tyrosine recombinase XerS -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX dprA positive effect
  comX comGG positive effect
  comX comEA positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGA positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31949.05 Da        Isoelectric Point: 9.9592

>NTDB_id=599 KW2_RS05940 WP_021037268.1 1241245..1242093(+) (dprA) [Lactococcus lactis subsp. cremoris KW2]
MITNFDLFRWKKAGMTNLGVNKLLKFFRKYDRKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRADYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKSVQKIVTELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKYQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide


Download         Length: 849 bp        

>NTDB_id=599 KW2_RS05940 WP_021037268.1 1241245..1242093(+) (dprA) [Lactococcus lactis subsp. cremoris KW2]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCGCAAATATGATAGAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAAGATGTAAAAAAACTAAGAGCAGACTATAAAAAATTCTCCTCCTTTTCTATTCTTGATGACCTTTAT
CCCGAGCGCCTAAGAGAAATCTATAATCCACCTGTCTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAATCTGTCCAAAAAATTGTGACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAATATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTCCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGAGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGCAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTTTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(69-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Streptococcus mutans UA159

58.273

99.286

0.579
  dprA/cilB/dalA Streptococcus mitis SK321

56.071

99.291

0.557
  dprA/cilB/dalA Streptococcus pneumoniae D39

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae R6

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

55.714

99.291

0.553
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

55.714

99.291

0.553
  dprA Bacillus subtilis subsp. subtilis str. 168

42.105

94.326

0.397
  dprA Vibrio campbellii strain DS40M4

35.314

100

0.379
  dprA Legionella pneumophila strain ERS1305867

38.768

97.872

0.379
  dprA Haemophilus influenzae Rd KW20

39.259

95.745

0.376

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]