Detailed information    

experimental Experimentally validated

Overview

Name   comX   Type   Regulator
Locus tag   KW2_RS10975 Genome accession   NC_022369
Coordinates   2276371..2276862 (-) Length   163 a.a.
NCBI ID   WP_021037965.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. cremoris KW2     
Function   activation of late competence genes   
Competence regulation

Function

We constitutively overproduced ComX in a reporter strain of late competence phase activation and showed, by transcriptomic analyses, a ComX-dependent induction of all key competence genes.


Genomic Context

Location: 2271371..2281862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW2_RS10975 (kw2_2209) comX 2276371..2276862 (-) 492 WP_021037965.1 sigma-70 family RNA polymerase sigma factor Regulator
  KW2_RS10980 (kw2_2210) ezrA 2277128..2278858 (-) 1731 WP_011836066.1 septation ring formation regulator EzrA -
  KW2_RS10985 (kw2_2211) tsf 2279063..2280091 (-) 1029 WP_011677206.1 translation elongation factor Ts -
  KW2_RS10990 (kw2_2212) rpsB 2280213..2280980 (-) 768 WP_011836067.1 30S ribosomal protein S2 -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX comGG positive effect
  comX comEA positive effect
  comX ssbB positive effect
  comX comGD positive effect
  comX dprA positive effect
  comX comFA positive effect
  comX coiA positive effect
  comX comGB positive effect
  comX recA positive effect
  comX comFC positive effect
  comX comGE positive effect
  comX comGF positive effect
  comX comEC positive effect
  comX comGA positive effect
  comX comGC positive effect
  mecA comX negative effect
  clpC comX negative effect
  clpP comX negative effect

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 19742.55 Da        Isoelectric Point: 6.2273

>NTDB_id=585 KW2_RS10975 WP_021037965.1 2276371..2276862(-) (comX) [Lactococcus lactis subsp. cremoris KW2]
MTYYLEENEFEGLFSGMKPIIRKLMKQIRIKAWDIEDYYQEGMIILHHLLEENHPSTNIYTKFKVKYHQRLIDELRHSYA
KKRLHDHFVGLDIYECSDWIDAGGSTPESELVFNHLLVEVYEGLSAHYQELLVRQMRGEELTRMERYRLREKIKNILFSR
DDD

Nucleotide


Download         Length: 492 bp        

>NTDB_id=585 KW2_RS10975 WP_021037965.1 2276371..2276862(-) (comX) [Lactococcus lactis subsp. cremoris KW2]
ATGACATATTACTTGGAAGAAAATGAATTCGAAGGTTTATTTTCTGGAATGAAACCAATCATCAGAAAATTGATGAAACA
AATTCGAATCAAAGCATGGGACATAGAGGATTATTATCAAGAAGGAATGATTATTTTGCATCACCTTTTAGAAGAAAATC
ACCCATCCACTAATATTTATACAAAGTTCAAAGTAAAATATCATCAACGTTTGATTGATGAACTACGCCATAGCTACGCC
AAAAAACGGCTTCATGACCATTTTGTAGGTCTGGACATTTATGAATGTTCGGACTGGATAGATGCAGGAGGAAGTACCCC
TGAAAGCGAGCTTGTGTTCAATCATCTTTTAGTAGAAGTTTATGAAGGATTGAGCGCCCACTATCAGGAATTACTCGTGC
GTCAAATGAGAGGAGAAGAACTCACGCGAATGGAACGCTATCGGCTACGAGAAAAAATCAAAAATATACTATTTTCTCGA
GATGATGATTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX Lactococcus lactis subsp. lactis strain DGCC12653

75.46

100

0.755

Multiple sequence alignment    



References

[1] Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]