Detailed information NTG0000602

Experimentally validated

Overview

• Name: clpP
• Type: Regulator
• Locus tag: KW2_RS03060
• Genome accession: NC_022369
• Coordinates: 611602..612201 (-)
• Length: 199 a.a.
• NCBI ID: WP_011834647.1
• Uniprot ID: A0A166TF89
• Organism: Lactococcus lactis subsp. cremoris KW2
• Function: degradation of ComX Competence regulation

Function

MecA, ClpC and ClpP are involved in the proteolytic degradation of ComX, thereby play a significant role in the negative control of competence in strain KW2.

Genomic Context

Location: 606602..617201

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KW2_RS03050 (kw2_0612)	acnA	607758..610304 (+)	2547	WP_021036853.1	aconitate hydratase AcnA	-
KW2_RS03055 (kw2_0613)	icd	610324..611562 (+)	1239	WP_021036854.1	NADP-dependent isocitrate dehydrogenase	-
KW2_RS03060 (kw2_0614)	clpP	611602..612201 (-)	600	WP_011834647.1	ATP-dependent Clp protease proteolytic subunit	Regulator
KW2_RS03065 (kw2_0615)	-	612397..612927 (+)	531	WP_011675638.1	hypothetical protein	-
KW2_RS03070 (kw2_0616)	spx	613098..613496 (+)	399	WP_003129597.1	transcriptional regulator Spx	-
KW2_RS12310 (kw2_0617)	-	613647..613781 (-)	135	WP_014572996.1	hypothetical protein	-
KW2_RS03075 (kw2_0618)	-	613878..615041 (-)	1164	WP_021036855.1	hypothetical protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
clpP	comX	negative effect
comX	comGG	positive effect
comX	comEA	positive effect
comX	ssbB	positive effect
comX	comGD	positive effect
comX	dprA	positive effect
comX	comFA	positive effect
comX	coiA	positive effect
comX	comGB	positive effect
comX	recA	positive effect
comX	comFC	positive effect
comX	comGE	positive effect
comX	comGF	positive effect
comX	comEC	positive effect
comX	comGA	positive effect
comX	comGC	positive effect
mecA	comX	negative effect
clpC	comX	negative effect

Sequence

Protein

• Length: 199 a.a.
• Molecular weight: 22052.21 Da
• Isoelectric Point: 4.7423

>NTDB_id=602 KW2_RS03060 WP_011834647.1 611602..612201(-) (clpP) [Lactococcus lactis subsp. cremoris KW2]
MGYLVPTVIEQSSRGERAYDIYSRLLKDRIIMLTGPVEDGMANSIIAQLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVD
TMNFIKSDVQTIVMGMAASMGTIIASSGTKGKRFMLPNAEYLIHQPMGGAGQGTQQTDMAIVAEQLLKTRKRLEQILADN
SNRSLEQIHKDAERDHWMDAKETLEYGFIDEIMENNSLK

Nucleotide

• Length: 600 bp

>NTDB_id=602 KW2_RS03060 WP_011834647.1 611602..612201(-) (clpP) [Lactococcus lactis subsp. cremoris KW2]
ATGGGTTATTTAGTACCTACCGTTATTGAACAATCAAGCCGTGGTGAACGTGCTTATGATATTTATTCACGACTTTTAAA
AGACCGTATTATCATGCTGACAGGACCTGTTGAAGATGGTATGGCAAATTCTATCATTGCCCAACTTCTTTTCTTGGATG
CACAAGATAATACTAAAGACATTTATCTCTATGTAAACACACCTGGTGGTTCTGTATCAGCTGGGCTCGCTATCGTGGAT
ACAATGAACTTTATCAAATCGGATGTCCAAACTATCGTTATGGGAATGGCTGCTTCTATGGGAACAATCATCGCCTCAAG
CGGTACTAAGGGTAAACGATTTATGTTACCAAATGCTGAATACCTCATTCACCAACCAATGGGTGGTGCAGGTCAAGGTA
CTCAACAAACTGATATGGCAATCGTCGCCGAACAACTTTTGAAAACACGTAAACGTTTGGAACAAATCCTTGCTGATAAT
TCTAATCGCTCACTTGAACAAATTCACAAAGATGCTGAGCGTGACCATTGGATGGATGCCAAAGAAACTTTAGAATATGG
TTTCATTGATGAAATTATGGAAAATAATTCATTAAAATAA

Domains

Predicted by InterproScan.

• Clp protease (CLP_protease) (14-195)

3D structure

• AlphaFold DB: A0A166TF89

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpP	Lactococcus lactis subsp. lactis strain DGCC12653	99.497	100	0.995
clpP	Streptococcus thermophilus LMG 18311	86.735	100	0.867
clpP	Streptococcus thermophilus LMD-9	86.735	100	0.867
clpP	Streptococcus mutans UA159	87.245	98.492	0.859
clpP	Streptococcus pyogenes JRS4	85.714	100	0.857
clpP	Streptococcus pyogenes MGAS315	85.714	100	0.857
clpP	Streptococcus pneumoniae TIGR4	84.615	99.49	0.842
clpP	Streptococcus pneumoniae R6	84.615	99.49	0.842
clpP	Streptococcus pneumoniae D39	84.615	99.49	0.842
clpP	Streptococcus pneumoniae Rx1	84.615	99.49	0.842
clpP	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	56.995	99.485	0.567
clpP	Bacillus subtilis subsp. subtilis str. 168	57.895	96.447	0.558

References

• [1]: Blandine David et al. (2017) Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2. Applied And Environmental Microbiology 83(16):e01074-17. [PMID: 28625996]