Detailed information    

experimental Experimentally validated

Overview

Name   clpP   Type   Regulator
Locus tag   SPYM3_RS01720 Genome accession   NC_004070
Coordinates   325283..325873 (+) Length   196 a.a.
NCBI ID   WP_002985850.1    Uniprot ID   A0A9X8T4H8
Organism   Streptococcus pyogenes MGAS315     
Function   degradation of ComX   
Competence regulation

Function

The ClpP protease participates in the degradation of SigX, thus likely influencing transcription of genes downstream of sigX expression.


Genomic Context

Location: 320283..330873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM3_RS01705 (SpyM3_0284) - 321096..322541 (-) 1446 WP_011054217.1 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase -
  SPYM3_RS01710 (SpyM3_0285) - 322629..324263 (+) 1635 WP_011054218.1 putative polysaccharide biosynthesis protein -
  SPYM3_RS01715 (SpyM3_0286) upp 324431..325060 (+) 630 WP_002985854.1 uracil phosphoribosyltransferase -
  SPYM3_RS01720 (SpyM3_0287) clpP 325283..325873 (+) 591 WP_002985850.1 ATP-dependent Clp protease proteolytic subunit Regulator
  SPYM3_RS01725 (SpyM3_0288) - 326365..326640 (+) 276 WP_002985847.1 YlbG family protein -
  SPYM3_RS01730 (SpyM3_0289) tmk 326889..327524 (+) 636 WP_011054219.1 dTMP kinase -
  SPYM3_RS01735 (SpyM3_0290) - 327542..328417 (+) 876 WP_011054220.1 DNA polymerase III subunit delta' -
  SPYM3_RS10465 - 328436..328582 (+) 147 WP_227868520.1 hypothetical protein -
  SPYM3_RS01750 (SpyM3_0291) yabA 329081..329404 (+) 324 WP_011054221.1 DNA replication initiation control protein YabA -
  SPYM3_RS01755 (SpyM3_0292) rsmI 329409..330272 (+) 864 WP_011054222.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SPYM3_RS01760 (SpyM3_0293) - 330299..330691 (+) 393 WP_002985833.1 DUF5684 domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  clpP comX/sigX/comX1/sigX1 negative effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comR comX/sigX/comX1/sigX1 positive effect
  comR comX/sigX/comX2/sigX2 positive effect
  prx comR negative effect
  comS comX/sigX/comX1/sigX1 positive effect
  comS comX/sigX/comX2/sigX2 positive effect
  clpP comX/sigX/comX2/sigX2 negative effect
  comX/sigX/comX2/sigX2 late competence genes positive effect

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 21648.88 Da        Isoelectric Point: 5.2834

>NTDB_id=445 SPYM3_RS01720 WP_002985850.1 325283..325873(+) (clpP) [Streptococcus pyogenes MGAS315]
MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIAQLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMN
FIKADVQTIVMGMAASMGTVIASSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=445 SPYM3_RS01720 WP_002985850.1 325283..325873(+) (clpP) [Streptococcus pyogenes MGAS315]
ATGATTCCTGTTGTTATTGAACAAACTAGCCGTGGAGAACGTTCTTATGACATTTATTCACGTTTATTAAAAGACCGTAT
TATCATGTTAACAGGGCCTGTCGAAGACAACATGGCCAACTCTGTCATAGCGCAGCTCTTATTCCTTGACGCGCAAGACA
ACACGAAAGATATCTACCTATATGTCAACACACCAGGTGGCTCTGTCTCAGCAGGCTTGGCTATTGTCGATACCATGAAC
TTTATCAAGGCCGATGTGCAAACTATTGTCATGGGTATGGCAGCTTCTATGGGGACAGTCATTGCTTCATCAGGAACAAA
AGGCAAACGCTTTATGTTACCAAATGCAGAGTATATGATCCACCAGCCAATGGGTGGCACAGGCGGTGGCACGCAACAAA
CGGATATGGCTATCGCAGCAGAACATCTCTTAAAAACACGTCACCGTTTAGAAAAAATCTTAGCGCAAAATGCTGGGAAA
ACGATTAAACAAATTCATAAAGATGCTGAGCGTGATTATTGGATGAGTGCTGAAGAAACCTTGGCTTACGGTTTCATTGA
TGAAATCATGGAAAACAACGAACTAAAATAA

Domains

Predicted by InterproScan.

Clp protease (CLP_protease)

(11-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpP Streptococcus pyogenes JRS4

100

100

1
  clpP Streptococcus mutans UA159

90.816

100

0.908
  clpP Streptococcus pneumoniae R6

90.769

99.49

0.903
  clpP Streptococcus pneumoniae TIGR4

90.769

99.49

0.903
  clpP Streptococcus pneumoniae D39

90.769

99.49

0.903
  clpP Streptococcus pneumoniae Rx1

90.769

99.49

0.903
  clpP Streptococcus thermophilus LMD-9

89.796

100

0.898
  clpP Streptococcus thermophilus LMG 18311

89.796

100

0.898
  clpP Lactococcus lactis subsp. lactis strain DGCC12653

86.224

100

0.862
  clpP Lactococcus lactis subsp. cremoris KW2

85.714

100

0.857
  clpP Bacillus subtilis subsp. subtilis str. 168

57.143

100

0.571
  clpP Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

56.995

98.469

0.561

Multiple sequence alignment    



References

[1] Lauren Mashburn-Warren et al. (2012) The cryptic competence pathway in Streptococcus pyogenes is controlled by a peptide pheromone. Journal of Bacteriology 194(17):4589-600. [PMID: 22730123]