Detailed information    

experimental Experimentally validated

Overview

Name   prx   Type   Regulator
Locus tag   SPYM3_RS04945 Genome accession   NC_004070
Coordinates   977437..977625 (-) Length   62 a.a.
NCBI ID   WP_011054546.1    Uniprot ID   A0A5S4TJS4
Organism   Streptococcus pyogenes MGAS315     
Function   Inhibit ComR activation   
Competence regulation

Function

In vitro experiments demonstrate that Prx binds ComR directly and prevents the ComR-XIP complex from interacting with DNA. Mutations of prx in vivo caused increased expression of the late competence gene ssb when induced with XIP as compared to wild-type, and Prx orthologues are able to inhibit ComR activation by XIP in a reporter strain which lacks an endogenous prx. An X-ray crystal structure of Prx reveals a unique fold that implies a novel molecular mechanism to inhibit ComR.


Genomic Context

Location: 972437..982625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM3_RS04925 (SpyM3_0915) - 974237..974608 (+) 372 WP_002989617.1 GntR family transcriptional regulator -
  SPYM3_RS04930 (SpyM3_0916) - 974608..975306 (+) 699 WP_002984437.1 ABC transporter ATP-binding protein -
  SPYM3_RS04935 (SpyM3_0917) - 975316..976101 (+) 786 WP_002984433.1 hypothetical protein -
  SPYM3_RS04940 (SpyM3_0918) - 976232..976846 (-) 615 WP_002989607.1 TVP38/TMEM64 family protein -
  SPYM3_RS04945 (SpyM3_0919) prx 977437..977625 (-) 189 WP_011054546.1 hypothetical protein Regulator
  SPYM3_RS04950 (SpyM3_0920) entC3 977738..978520 (-) 783 WP_002988472.1 enterotoxin type C3 EntC3 -
  SPYM3_RS04955 (SpyM3_0921) - 978733..979857 (-) 1125 WP_011054547.1 Fic family protein -
  SPYM3_RS04960 (SpyM3_0922) - 979995..981203 (-) 1209 WP_011054548.1 glucosaminidase domain-containing protein -
  SPYM3_RS04970 (SpyM3_0923) - 981319..981546 (-) 228 WP_000609113.1 phage holin -
  SPYM3_RS04975 (SpyM3_0924) - 981543..981818 (-) 276 WP_002987582.1 DUF7365 family protein -
  SPYM3_RS04980 (SpyM3_0925) - 981828..982445 (-) 618 WP_010922094.1 DUF1366 domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  prx comR negative effect
  comR comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comR comX/sigX/comX1/sigX1 positive effect
  comS comX/sigX/comX1/sigX1 positive effect
  clpP comX/sigX/comX1/sigX1 negative effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comS comX/sigX/comX2/sigX2 positive effect
  clpP comX/sigX/comX2/sigX2 negative effect

Sequence

Protein


Download         Length: 62 a.a.        Molecular weight: 7226.17 Da        Isoelectric Point: 3.9947

>NTDB_id=551 SPYM3_RS04945 WP_011054546.1 977437..977625(-) (prx) [Streptococcus pyogenes MGAS315]
MLTYDEFKQAIDDGYIVGDTVMIVRKNGQIFDYVLPHEEARNGEVVTEEKVEEVMVELDYIK

Nucleotide


Download         Length: 189 bp        

>NTDB_id=551 SPYM3_RS04945 WP_011054546.1 977437..977625(-) (prx) [Streptococcus pyogenes MGAS315]
ATGCTAACATACGACGAATTTAAGCAAGCTATTGATGACGGATATATCGTAGGAGACACAGTTATGATCGTGCGTAAAAA
CGGACAGATTTTTGATTATGTGTTGCCGCATGAGGAAGCGAGAAATGGAGAAGTTGTGACAGAGGAGAAGGTGGAAGAAG
TGATGGTGGAATTAGACTATATCAAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  prx Streptococcus pyogenes MGAS315

84.746

95.161

0.806
  prx Streptococcus pyogenes MGAS8232

84.483

93.548

0.79
  prx Streptococcus pyogenes MGAS315

82.759

93.548

0.774
  prx Streptococcus pyogenes MGAS315

77.966

95.161

0.742
  prx Streptococcus pyogenes MGAS315

87.805

68.333

0.6
  prx Streptococcus pyogenes MGAS315

76.19

70

0.533

Multiple sequence alignment    



References

[1] Lauren Mashburn-Warren et al. (2018) The conserved mosaic prophage protein paratox inhibits the natural competence regulator ComR in Streptococcus. Scientific Reports 8(1):16535. [PMID: 30409983]