Detailed information NTG0000443

Experimentally validated

Overview

• Name: comR
• Type: Regulator
• Locus tag: SPYM3_RS00325
• Genome accession: NC_004070
• Coordinates: 53474..54385 (+)
• Length: 303 a.a.
• NCBI ID: WP_011054099.1
• Uniprot ID: A0A5S4TJF2
• Organism: Streptococcus pyogenes MGAS315
• Function: activate transcription of comX Competence regulation

Function

The S. pyogenes wild type and comR mutant resembled the S. mutans comR mutant, where no DNA was transported. The sigX reporter studies indicate that both comR alleles in S. pyogenes are capable of activating the transcription of the alternative sigma factor gene sigX upon addition of XIP.

Genomic Context

Location: 48474..59385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYM3_RS00305 (SpyM3_0027)	purE	48770..49261 (+)	492	WP_030127619.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
SPYM3_RS00310 (SpyM3_0028)	purK	49248..50321 (+)	1074	WP_032461293.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
SPYM3_RS00315 (SpyM3_0029)	-	50342..51682 (+)	1341	WP_002986685.1	hypothetical protein	-
SPYM3_RS00320 (SpyM3_0030)	purB	52051..53343 (+)	1293	WP_011054098.1	adenylosuccinate lyase	-
SPYM3_RS00325 (SpyM3_0031)	comR	53474..54385 (+)	912	WP_011054099.1	helix-turn-helix domain-containing protein	Regulator
SPYM3_RS10450	comS	54476..54574 (+)	99	WP_020833188.1	quorum-sensing system DWW-type pheromone	Regulator
SPYM3_RS00330 (SpyM3_0032)	ruvB	54605..55603 (+)	999	WP_011054100.1	Holliday junction branch migration DNA helicase RuvB	-
SPYM3_RS00335 (SpyM3_0033)	-	55741..56178 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
SPYM3_RS00340 (SpyM3_0034)	-	56201..56602 (+)	402	WP_011017240.1	MORN repeat-containing protein	-
SPYM3_RS00345 (SpyM3_0035)	-	56599..58374 (+)	1776	WP_011054101.1	acyltransferase family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comR	comX/sigX/comX2/sigX2	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comR	comX/sigX/comX1/sigX1	positive effect
prx	comR	negative effect
comS	comX/sigX/comX1/sigX1	positive effect
comS	comX/sigX/comX2/sigX2	positive effect
clpP	comX/sigX/comX1/sigX1	negative effect
clpP	comX/sigX/comX2/sigX2	negative effect

Sequence

Protein

• Length: 303 a.a.
• Molecular weight: 35896.55 Da
• Isoelectric Point: 4.9324

>NTDB_id=443 SPYM3_RS00325 WP_011054099.1 53474..54385(+) (comR) [Streptococcus pyogenes MGAS315]
MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSIPSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELK
YLILRTPTYMDDGKLQVREEQFDEIFEDYYDKLPEEEKVAVDIIQAKFEVYQTGDINFGYSILKEFLPQLKRKSIYNLNE
LLLIDLYLIILVVSHFSDDIFDVQFYEEITESMLKQHNNLSLEDLFLLNNILLSCADTYIRLKMFGHLKETLQLSHFIMS
TIQDFQKMPMYCMYEWKLSIFYLKDINRARNYFEQSILFTQMTGDTYLVQKLRGEWNKDIHYI

Nucleotide

• Length: 912 bp

>NTDB_id=443 SPYM3_RS00325 WP_011054099.1 53474..54385(+) (comR) [Streptococcus pyogenes MGAS315]
ATGTTAGAACATTTTGGTGGAAAAGTAAAAGTGTTAAGACTTGAAAAGAGGATTAGTCGCGAGGACTTGTGTGGGGATGA
GTCTGAACTTTCTGTTCGTCAATTAGCACGGATAGAACTAGGTCAATCCATACCAAGTTTAAGTAAGGTTATTTTTATTG
CAAAAGCCTTAAACGTTAGTGTCGGTTACTTAACTGATGGTGCTGATTTAGAACTACCTAAGCGTTACAAAGAATTAAAA
TACCTTATCTTAAGGACACCAACTTACATGGATGATGGAAAATTACAAGTACGAGAAGAGCAGTTTGATGAAATTTTTGA
GGATTATTATGATAAATTACCAGAGGAAGAGAAAGTTGCTGTTGATATTATCCAAGCAAAATTTGAAGTTTATCAAACCG
GAGATATTAATTTCGGATATAGTATTTTAAAAGAATTTCTACCACAGCTAAAAAGAAAATCTATCTATAATTTAAATGAA
TTATTACTTATAGATTTGTATTTGATTATTCTGGTAGTTTCTCATTTTTCAGATGATATATTTGATGTACAGTTTTATGA
AGAAATAACAGAGAGCATGTTAAAACAACACAATAATTTATCATTGGAAGATTTATTTTTACTGAATAATATCTTATTAT
CTTGTGCGGACACTTATATTCGTTTGAAGATGTTCGGTCATCTAAAAGAAACATTACAACTTAGCCACTTTATAATGTCA
ACTATTCAAGATTTTCAAAAAATGCCTATGTATTGTATGTATGAATGGAAGCTATCTATTTTTTATTTGAAAGATATTAA
CCGAGCACGGAATTACTTTGAACAGTCTATTTTATTTACTCAAATGACAGGAGATACATATTTAGTTCAAAAATTACGGG
GAGAATGGAATAAAGATATTCATTATATTTAA

Domains

Predicted by InterproScan.

• ComR tetratricopeptide (ComR_TPR) (73-296)

• Helix-turn-helix (HTH_3) (12-64)

3D structure

• AlphaFold DB: A0A5S4TJF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comR	Streptococcus pyogenes MGAS8232	58.472	99.34	0.581
comR	Streptococcus infantarius subsp. infantarius ATCC BAA-102	46.441	100	0.469
comR	Streptococcus mutans UA159	47.157	98.68	0.465
comR	Streptococcus suis P1/7	37.374	99.331	0.371
comR	Streptococcus suis 05ZYH33	37.374	98.02	0.366

References

• [1]: Lauren Mashburn-Warren et al. (2012) The cryptic competence pathway in Streptococcus pyogenes is controlled by a peptide pheromone. Journal of Bacteriology 194(17):4589-600. [PMID: 22730123]