Detailed information NTG0000451

Experimentally validated

Overview

• Name: comR
• Type: Regulator
• Locus tag: SPYM18_RS00325
• Genome accession: NC_003485
• Coordinates: 53359..54270 (+)
• Length: 303 a.a.
• NCBI ID: WP_002986681.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes MGAS8232
• Function: activate transcription of comX Competence regulation

Function

The S. pyogenes wild type and comR mutant resembled the S. mutans comR mutant, where no DNA was transported. The sigX reporter studies indicate that both comR alleles in S. pyogenes are capable of activating the transcription of the alternative sigma factor gene sigX upon addition of XIP.

Genomic Context

Location: 48359..59270

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYM18_RS00305 (spyM18_0033)	purE	48652..49143 (+)	492	WP_023079557.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
SPYM18_RS00310 (spyM18_0034)	purK	49130..50203 (+)	1074	WP_023079558.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
SPYM18_RS00315 (spyM18_0035)	-	50224..51564 (+)	1341	WP_011017237.1	hypothetical protein	-
SPYM18_RS00320 (spyM18_0037)	purB	51933..53225 (+)	1293	WP_011017238.1	adenylosuccinate lyase	-
SPYM18_RS00325 (spyM18_0038)	comR	53359..54270 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
SPYM18_RS10730	comS	54355..54450 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
SPYM18_RS00330 (spyM18_0039)	ruvB	54497..55495 (+)	999	WP_023079556.1	Holliday junction branch migration DNA helicase RuvB	-
SPYM18_RS00335 (spyM18_0040)	-	55633..56070 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
SPYM18_RS00340 (spyM18_0041)	-	56093..56494 (+)	402	WP_011017240.1	MORN repeat-containing protein	-
SPYM18_RS00345 (spyM18_0042)	-	56491..58266 (+)	1776	WP_011017241.1	acyltransferase family protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comR	comX/sigX/comX1/sigX1	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comR	comX/sigX/comX2/sigX2	positive effect
prx	comR	negative effect
comS	comX/sigX/comX1/sigX1	positive effect
comS	comX/sigX/comX2/sigX2	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect

Sequence

Protein

• Length: 303 a.a.
• Molecular weight: 35757.27 Da
• Isoelectric Point: 4.4858

>NTDB_id=451 SPYM18_RS00325 WP_002986681.1 53359..54270(+) (comR) [Streptococcus pyogenes MGAS8232]
MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSIPSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELK
YLILRTPTYMDDGKLQVREEQFDEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
LILLELYLAYLDIEGMDGQYSDKIFYDSLLDNLSEQFEQFELDELFIVNKIIIDISSLSLKNNRLDNLEKAIEMSQKIMA
KIQDWNRMPILKLIEWKYFLIKQKDIIKAEQSFMKACLFAQMTADQYLENKLIQEWEKDVKSY

Nucleotide

• Length: 912 bp

>NTDB_id=451 SPYM18_RS00325 WP_002986681.1 53359..54270(+) (comR) [Streptococcus pyogenes MGAS8232]
ATGTTAGAACATTTTGGTGGAAAAGTAAAAGTGTTAAGACTTGAAAAGAGGATTAGTCGCGAGGACTTGTGTGGGGATGA
GTCTGAACTTTCTGTTCGTCAATTAGCACGGATAGAACTAGGTCAATCCATACCAAGTTTAAGTAAGGTTATTTTTATTG
CAAAAGCCTTAAACGTTAGTGTCGGTTACTTAACTGATGGTGCTGATTTAGAACTACCTAAGCGTTACAAAGAATTAAAA
TACCTTATCTTAAGGACACCAACTTACATGGATGATGGAAAATTACAAGTACGAGAAGAGCAGTTTGATGAAATTTTTGA
GGATTATTATGATAAATTACCAGAGGAAGAGAAAATAATCATTGATTGTTTACAGGCAACTTTAGATACTTTATTGAGTG
AGAATACTAACTTTGGCATTGACTTACTTCAAGAATATTTTAATCAAATAAAGACTAAGGTACGCTTTAGGCAAAATGAT
TTAATACTTCTAGAATTATATTTAGCTTATCTTGATATTGAGGGAATGGATGGACAATATTCAGATAAGATTTTTTATGA
TTCTTTATTAGATAATCTATCAGAACAATTTGAACAATTTGAATTAGATGAATTATTTATAGTTAATAAGATTATCATTG
ATATTTCATCACTAAGCTTAAAAAATAACCGACTTGATAATTTAGAAAAAGCGATTGAAATGAGTCAAAAAATTATGGCG
AAAATTCAAGATTGGAATAGAATGCCTATTTTAAAACTAATAGAATGGAAATATTTTTTAATCAAACAAAAAGATATAAT
AAAAGCAGAACAATCATTTATGAAGGCATGTTTGTTCGCCCAAATGACAGCTGATCAGTATTTGGAAAACAAGTTAATTC
AAGAGTGGGAAAAAGATGTTAAAAGTTATTAA

Domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (12-64)

• ComR tetratricopeptide (ComR_TPR) (73-296)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comR	Streptococcus pyogenes MGAS315	58.472	99.34	0.581
comR	Streptococcus infantarius subsp. infantarius ATCC BAA-102	51.37	100	0.514
comR	Streptococcus mutans UA159	46.358	99.67	0.462

References

• [1]: Lauren Mashburn-Warren et al. (2012) The cryptic competence pathway in Streptococcus pyogenes is controlled by a peptide pheromone. Journal of Bacteriology 194(17):4589-600. [PMID: 22730123]