Detailed information    

experimental Experimentally validated

Overview

Name   comR   Type   Regulator
Locus tag   STRINF_RS01865 Genome accession   NZ_DS572685
Coordinates   23851..24729 (+) Length   292 a.a.
NCBI ID   WP_174221231.1    Uniprot ID   -
Organism   Streptococcus infantarius subsp. infantarius ATCC BAA-102     
Function   activate transcription of comX   
Competence regulation

Function

Deletion of comR comS eliminated both endogenous expression of competence and response to synthetic peptide. ComR is a key regulator of competence gene expression in S. infantarius and that it is required for sensing a ComS product.


Genomic Context

Location: 18851..29729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STRINF_RS01840 (STRINF_00388) purD 18910..20172 (+) 1263 WP_006531303.1 phosphoribosylamine--glycine ligase -
  STRINF_RS01845 (STRINF_00389) - 20204..20610 (+) 407 Protein_16 DUF523 domain-containing protein -
  STRINF_RS01850 (STRINF_00390) purE 20610..21098 (+) 489 WP_006531305.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  STRINF_RS01855 (STRINF_00391) purK 21085..22176 (+) 1092 WP_006531306.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  STRINF_RS10415 - 22179..22274 (+) 96 Protein_19 phosphoribosylaminoimidazole carboxylase -
  STRINF_RS01860 (STRINF_00392) purB 22364..23662 (+) 1299 WP_006531307.1 adenylosuccinate lyase -
  STRINF_RS01865 (STRINF_00393) comR 23851..24729 (+) 879 WP_174221231.1 helix-turn-helix domain-containing protein Regulator
  - comS 24832..24876 (+) 45 - - Regulator
  STRINF_RS01870 (STRINF_00395) ruvB 24931..25929 (+) 999 WP_006531310.1 Holliday junction branch migration DNA helicase RuvB -
  STRINF_RS10420 (STRINF_00396) - 26102..26308 (+) 207 WP_006531311.1 DUF7010 family protein -
  STRINF_RS10865 (STRINF_00397) - 26268..26633 (+) 366 WP_398577259.1 DUF7010 family protein -
  STRINF_RS01880 (STRINF_00398) - 26872..27303 (+) 432 Protein_25 low molecular weight protein-tyrosine-phosphatase -
  STRINF_RS01885 (STRINF_00399) - 27321..27701 (+) 381 WP_006531314.1 membrane protein -
  STRINF_RS01890 (STRINF_00400) - 27698..29491 (+) 1794 WP_006531315.1 acyltransferase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comE/comE1 positive effect
  comR comC/comC1 positive effect
  comS comE/comE1 positive effect
  comS comC/comC1 positive effect

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 34436.27 Da        Isoelectric Point: 4.5999

>NTDB_id=555 STRINF_RS01865 WP_174221231.1 23851..24729(+) (comR) [Streptococcus infantarius subsp. infantarius ATCC BAA-102]
MREEKEISREEFCGDETELSVRQLARIELNQSIPNLSKASFIANRLGVKLGTLTDGDSLELPKRYKELKYLLLRTPTYGD
QVRLDRKNDYFDEIAEVFYDVIPEEERLIIDCLQSKFDVHFSEDVNFGEGILNDYFGQVNRKKVFTINDLILIDLYFACL
SSAKKFEGIYSLDFYDDLMKRLVNQKHVSPETDLILNNVLLNNIDLAFQFKREYYIERVITISEKIMTEIHDFQRRPILS
LVEWKYYLKLKHDFALAEQSFTNATLFARLVGDTYLENKLKEEWQLDIDAVE

Nucleotide


Download         Length: 879 bp        

>NTDB_id=555 STRINF_RS01865 WP_174221231.1 23851..24729(+) (comR) [Streptococcus infantarius subsp. infantarius ATCC BAA-102]
ATGCGGGAGGAAAAGGAAATCTCACGTGAAGAATTCTGTGGGGATGAAACAGAATTATCGGTAAGACAATTAGCTAGAAT
TGAGTTGAACCAATCAATACCCAATCTCAGTAAAGCAAGCTTTATTGCCAATCGTCTGGGAGTGAAATTAGGTACTTTAA
CTGATGGTGACAGTTTAGAATTACCTAAGCGGTATAAAGAGTTGAAGTATTTACTTCTTCGAACACCTACATATGGAGAC
CAGGTACGACTTGATCGAAAAAATGACTATTTTGATGAGATTGCTGAAGTATTTTATGATGTTATTCCAGAAGAAGAACG
GTTAATCATTGATTGTTTACAATCAAAATTCGATGTTCATTTTAGTGAAGATGTCAATTTTGGAGAGGGTATTTTAAATG
ATTATTTTGGTCAAGTTAATCGAAAGAAAGTTTTTACCATTAATGATTTAATTTTGATTGATCTTTATTTTGCTTGTCTT
TCTTCTGCTAAGAAGTTTGAAGGAATTTATAGTTTGGATTTTTACGATGATTTGATGAAAAGGTTGGTAAATCAAAAGCA
TGTTTCACCTGAGACTGATTTAATTTTAAATAATGTATTGTTAAATAATATTGACTTAGCATTCCAATTTAAGCGAGAAT
ATTATATTGAGCGTGTTATTACAATCAGTGAAAAAATTATGACAGAGATTCATGATTTTCAACGTCGTCCGATTTTAAGT
TTGGTGGAGTGGAAGTATTATTTGAAACTTAAACATGACTTTGCTTTAGCAGAACAGTCATTTACGAATGCAACATTATT
TGCACGATTGGTTGGTGATACTTATTTAGAAAATAAACTTAAAGAAGAATGGCAACTTGATATTGATGCTGTGGAGTAG

Domains

Predicted by InterproScan.

ComR tetratricopeptide (ComR_TPR)

(62-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR Streptococcus mutans UA159

53.125

98.63

0.524
  comR Streptococcus pyogenes MGAS8232

51.37

100

0.514
  comR Streptococcus pyogenes MGAS315

46.441

100

0.469
  comR Streptococcus suis D9

36.86

100

0.37
  comR Streptococcus suis P1/7

37.153

98.63

0.366
  comR Streptococcus suis 05ZYH33

37.153

98.63

0.366

Multiple sequence alignment    



References

[1] Donald A Morrison et al. (2013) Competence for natural genetic transformation in the Streptococcus bovis group streptococci S. infantarius and S. macedonicus. Journal of Bacteriology 195(11):2612-20. [PMID: 23543718]