Detailed information    

experimental Experimentally validated

Overview

Name   comE/comE1   Type   Regulator
Locus tag   H702_RS02345 Genome accession   NZ_AUZH01000012
Coordinates   5873..6604 (+) Length   243 a.a.
NCBI ID   WP_039696227.1    Uniprot ID   A0A091BWZ4
Organism   Streptococcus equinus JB1     
Function   activate transcription of early competence genes   
Competence regulation

Function

The transcript level of comED was decreased by the disruption of comCD, suggesting that the transcription of comED might be stimulated by ComC.


Genomic Context

Location: 873..11604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H702_RS02320 (H702_02400) comA/nlmT 1410..3557 (-) 2148 WP_039696223.1 peptide cleavage/export ABC transporter Regulator
  H702_RS09975 (H702_02405) - 3797..3961 (+) 165 WP_074799080.1 Blp family class II bacteriocin -
  H702_RS02325 (H702_02410) - 4003..4212 (+) 210 WP_039696224.1 hypothetical protein -
  H702_RS02335 (H702_02420) - 4472..5086 (+) 615 WP_052071090.1 thioredoxin fold domain-containing protein -
  H702_RS02345 (H702_02430) comE/comE1 5873..6604 (+) 732 WP_039696227.1 response regulator transcription factor Regulator
  H702_RS10470 comD/comD1 7219..7941 (+) 723 WP_236726575.1 sensor histidine kinase Regulator
  H702_RS10235 (H702_02440) comC/comC1 7951..8094 (-) 144 WP_160172983.1 hypothetical protein Regulator
  H702_RS02355 (H702_02445) - 8358..8507 (+) 150 WP_039696228.1 hypothetical protein -
  H702_RS02360 (H702_02450) - 8520..8825 (+) 306 WP_039696229.1 bacteriocin immunity protein -
  H702_RS02365 (H702_02455) - 8853..9146 (+) 294 WP_039696231.1 bacteriocin immunity protein -
  H702_RS02375 (H702_02465) comD/comD2 9933..10880 (+) 948 WP_160172984.1 sensor histidine kinase Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comE/comE1 positive effect
  comS comE/comE1 positive effect
  comR comC/comC1 positive effect
  comS comC/comC1 positive effect

Sequence

Protein


Download         Length: 243 a.a.        Molecular weight: 28168.08 Da        Isoelectric Point: 6.2472

>NTDB_id=542 H702_RS02345 WP_039696227.1 5873..6604(+) (comE/comE1) [Streptococcus equinus JB1]
MLDIFILEDNITQQFRIERQIETIMERNHWEYQRLEAFAASKDIIKKADGQGNHQVFFLDLEIKGDEKQGLDVARSIREK
DPTAIIVFVTTHSEFMLLTYQSLVGAIDFIDKNMNDEAFGGRIELCLKEALKYQTGSFGENSYLFETAKARIRVAYDDIL
YFETSPTVHRVILHTKTGQTEFYGTIAEVAKSDKRLLKCHRSFVINVNNVTEFDKSTRTAYFENGSYCQVSRDKVKKLMA
EMR

Nucleotide


Download         Length: 732 bp        

>NTDB_id=542 H702_RS02345 WP_039696227.1 5873..6604(+) (comE/comE1) [Streptococcus equinus JB1]
ATGTTAGATATTTTTATCCTTGAGGATAATATCACGCAACAATTTCGAATCGAACGTCAGATTGAGACGATTATGGAAAG
GAACCACTGGGAGTATCAGCGTTTAGAGGCTTTTGCGGCATCTAAGGATATTATAAAAAAGGCAGATGGGCAGGGAAATC
ACCAGGTTTTCTTTCTTGATTTAGAAATCAAAGGGGATGAAAAGCAAGGCTTAGATGTTGCTAGATCTATTCGTGAAAAA
GATCCGACAGCCATTATTGTCTTTGTGACAACCCATTCTGAGTTTATGCTTCTAACTTATCAATCACTAGTTGGTGCGAT
TGATTTTATTGATAAAAATATGAATGATGAAGCCTTTGGAGGGCGCATTGAATTGTGCTTGAAAGAGGCATTGAAATATC
AAACTGGTAGTTTTGGTGAGAATTCTTACCTGTTTGAAACAGCAAAAGCAAGAATCCGCGTGGCTTATGATGACATTTTG
TATTTTGAAACATCGCCAACCGTGCACCGTGTGATTTTACACACTAAAACAGGTCAAACAGAGTTTTATGGCACAATTGC
TGAGGTAGCCAAATCAGATAAACGTTTGTTGAAATGTCACCGCTCATTCGTGATTAATGTTAATAATGTCACTGAATTTG
ATAAAAGCACCCGAACAGCTTATTTCGAAAACGGCTCTTACTGCCAAGTCTCAAGAGACAAAGTCAAAAAACTGATGGCA
GAAATGAGGTGA

Domains

Predicted by InterproScan.

LytTr DNA-binding domain (LytTR)

(152-240)

Response regulator receiver domain (Response_reg)

(4-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A091BWZ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE/blpR Streptococcus mutans UA159

46.888

99.177

0.465
  comE/comE2 Streptococcus equinus JB1

45.041

99.588

0.449

Multiple sequence alignment    



References

[1] Narito Asanuma et al. (2010) Involvement of two-component signal transduction system, ComDE, in the regulation of growth and genetic transformation, in the ruminal bacterium Streptococcus bovis. Anaerobe 16(4):405-11. [PMID: 20478389]