Detailed information NTG0000541

Experimentally validated

Overview

• Name: comD/comD1
• Type: Regulator
• Locus tag: H702_RS10470
• Genome accession: NZ_AUZH01000012
• Coordinates: 7219..7941 (+)
• Length: 240 a.a.
• NCBI ID: WP_236726575.1
• Uniprot ID: -
• Organism: Streptococcus equinus JB1
• Function: phosphorylation of ComE Competence regulation

Function

The transformation frequency was decreased by the disruption of comCD. Addition of recombinant ComC to S. bovis cultures increased the growth rate and transformation frequency.

Genomic Context

Location: 2219..12941

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H702_RS09975 (H702_02405)	-	3797..3961 (+)	165	WP_074799080.1	Blp family class II bacteriocin	-
H702_RS02325 (H702_02410)	-	4003..4212 (+)	210	WP_039696224.1	hypothetical protein	-
H702_RS02335 (H702_02420)	-	4472..5086 (+)	615	WP_052071090.1	thioredoxin fold domain-containing protein	-
H702_RS02345 (H702_02430)	comE/comE1	5873..6604 (+)	732	WP_039696227.1	response regulator transcription factor	Regulator
H702_RS10470	comD/comD1	7219..7941 (+)	723	WP_236726575.1	sensor histidine kinase	Regulator
H702_RS10235 (H702_02440)	comC/comC1	7951..8094 (-)	144	WP_160172983.1	hypothetical protein	Regulator
H702_RS02355 (H702_02445)	-	8358..8507 (+)	150	WP_039696228.1	hypothetical protein	-
H702_RS02360 (H702_02450)	-	8520..8825 (+)	306	WP_039696229.1	bacteriocin immunity protein	-
H702_RS02365 (H702_02455)	-	8853..9146 (+)	294	WP_039696231.1	bacteriocin immunity protein	-
H702_RS02375 (H702_02465)	comD/comD2	9933..10880 (+)	948	WP_160172984.1	sensor histidine kinase	Regulator
H702_RS02380 (H702_02470)	comD/comD3	10877..12253 (+)	1377	WP_039696235.1	sensor histidine kinase	Regulator

Sequence

Protein

• Length: 240 a.a.
• Molecular weight: 27430.16 Da
• Isoelectric Point: 5.4406

>NTDB_id=541 H702_RS10470 WP_236726575.1 7219..7941(+) (comD/comD1) [Streptococcus equinus JB1]
MNRSYQNRLEKEIASAREQELRYLSAYSKKVEGLYEELRAFRHDYANILVSLKEGIDQDDMGLVRKIYDSVLEDSADFIR
NSKFNIGRLVNIDDDAIKSLLSAKFLEAEASNIEVDLEVKDKIGAPNIPLLDYIRLLAILCDNAIEAALEAKKPAITIAC
FYQDDDYVLIVDNTTKEESVPVNLIYQKDYSSKGYGRGIGLTTIHQMMRKYSNLSVQTNSKNYHFSQTIRIKNHSTKIVE

Nucleotide

• Length: 723 bp

>NTDB_id=541 H702_RS10470 WP_236726575.1 7219..7941(+) (comD/comD1) [Streptococcus equinus JB1]
ATGAACCGTTCTTATCAAAATAGACTTGAAAAAGAGATTGCTAGTGCCCGTGAGCAAGAATTAAGATATTTATCAGCTTA
TAGTAAGAAAGTTGAGGGACTATACGAGGAGTTACGTGCCTTTCGTCATGATTATGCTAATATCTTAGTCAGCTTAAAAG
AGGGAATTGATCAAGATGATATGGGACTAGTTCGAAAAATCTACGATTCTGTTCTAGAGGATTCGGCGGATTTTATTCGA
AATAGCAAATTTAACATTGGGCGCTTGGTTAATATTGATGACGATGCCATAAAGAGTCTCTTATCTGCTAAGTTTTTAGA
AGCAGAAGCAAGTAATATTGAAGTTGATTTAGAAGTTAAAGATAAAATAGGAGCGCCAAACATTCCGCTACTAGATTATA
TTCGTTTGTTGGCTATTTTGTGTGATAATGCTATTGAAGCAGCGCTTGAAGCAAAAAAACCAGCCATCACGATTGCTTGT
TTTTATCAAGATGATGACTATGTATTAATTGTAGATAATACGACCAAGGAAGAGAGTGTTCCAGTTAATTTGATTTATCA
AAAAGATTATTCAAGTAAAGGATATGGTCGAGGTATTGGGCTAACCACGATTCATCAGATGATGCGAAAATATTCCAATC
TTTCTGTTCAAACAAATAGTAAAAATTACCATTTCAGCCAAACTATTCGTATAAAAAATCATTCGACTAAAATTGTCGAA
TGA

Domains

Predicted by InterproScan.

• GHKL domain (HATPase_c_5) (131-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comD/comD3	Streptococcus equinus JB1	50.216	96.25	0.483
comD/comD2	Streptococcus equinus JB1	49.784	96.25	0.479
comD/blpH	Streptococcus mutans UA159	42.291	94.583	0.4

References

• [1]: Narito Asanuma et al. (2010) Involvement of two-component signal transduction system, ComDE, in the regulation of growth and genetic transformation, in the ruminal bacterium Streptococcus bovis. Anaerobe 16(4):405-11. [PMID: 20478389]