Detailed information NTG0000543

Experimentally validated

Overview

• Name: comD/comD2
• Type: Regulator
• Locus tag: H702_RS02375
• Genome accession: NZ_AUZH01000012
• Coordinates: 9933..10880 (+)
• Length: 315 a.a.
• NCBI ID: WP_160172984.1
• Uniprot ID: -
• Organism: Streptococcus equinus JB1
• Function: phosphorylation of ComE Competence regulation

Function

The transformation frequency was decreased by the disruption of comCD. Addition of recombinant ComC to S. bovis cultures increased the growth rate and transformation frequency.

Genomic Context

Location: 4933..15880

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H702_RS02345 (H702_02430)	comE/comE1	5873..6604 (+)	732	WP_039696227.1	response regulator transcription factor	Regulator
H702_RS10470	comD/comD1	7219..7941 (+)	723	WP_236726575.1	sensor histidine kinase	Regulator
H702_RS10235 (H702_02440)	comC/comC1	7951..8094 (-)	144	WP_160172983.1	hypothetical protein	Regulator
H702_RS02355 (H702_02445)	-	8358..8507 (+)	150	WP_039696228.1	hypothetical protein	-
H702_RS02360 (H702_02450)	-	8520..8825 (+)	306	WP_039696229.1	bacteriocin immunity protein	-
H702_RS02365 (H702_02455)	-	8853..9146 (+)	294	WP_039696231.1	bacteriocin immunity protein	-
H702_RS02375 (H702_02465)	comD/comD2	9933..10880 (+)	948	WP_160172984.1	sensor histidine kinase	Regulator
H702_RS02380 (H702_02470)	comD/comD3	10877..12253 (+)	1377	WP_039696235.1	sensor histidine kinase	Regulator
H702_RS02385 (H702_02475)	comE/comE2	12914..13648 (+)	735	WP_052071091.1	LytR/AlgR family response regulator transcription factor	Regulator
H702_RS02395 (H702_02485)	rsgA	13966..14838 (+)	873	WP_039696236.1	ribosome small subunit-dependent GTPase A	-
H702_RS02400 (H702_02490)	rpe	14845..15507 (+)	663	WP_006531895.1	ribulose-phosphate 3-epimerase	-

Sequence

Protein

• Length: 315 a.a.
• Molecular weight: 36106.15 Da
• Isoelectric Point: 4.8934

>NTDB_id=543 H702_RS02375 WP_160172984.1 9933..10880(+) (comD/comD2) [Streptococcus equinus JB1]
MYYLVSRWLNIDISNVSQSLKKHIEPKIFNIINILMVSYFLFMDAIAIVKPFHNADNDAIRGIVAFIYLILLFVFLFYLN
FRHGQEQKNELLRNQEMELAALANYSKHVESLYEEVRSFRHDYANVLMSLKMGIDQGNLDDIKKIYDEITADSTKLVKNN
KFDLTRLANITDSGVKSLMSAKFLHAENQGIENSLEVSEKMGTPSIPLISYIRILSILFDNAIEGALESAEPKISVANFY
QDGDFVFMIENSSKEKSVDLGKIFERGYSTRGENRGLGLATLMDFQDDYENLSVETHSSDYTFTQVVRIYEGDSR

Nucleotide

• Length: 948 bp

>NTDB_id=543 H702_RS02375 WP_160172984.1 9933..10880(+) (comD/comD2) [Streptococcus equinus JB1]
GTGTATTATCTGGTTTCACGATGGTTAAATATTGATATTTCAAATGTAAGTCAAAGTTTAAAAAAACACATCGAGCCAAA
AATTTTTAATATTATTAATATCTTGATGGTCTCTTATTTTTTATTCATGGATGCCATTGCCATTGTAAAACCTTTTCATA
ATGCTGATAATGATGCAATAAGGGGAATTGTTGCTTTCATTTATCTCATCCTTCTTTTTGTCTTTCTCTTCTATCTCAAT
TTCCGTCATGGGCAAGAGCAGAAAAACGAACTGCTTCGCAATCAGGAGATGGAGCTTGCGGCACTTGCAAATTATAGCAA
GCACGTGGAGAGTTTGTATGAGGAAGTACGCAGTTTCAGGCATGATTACGCTAACGTCCTGATGAGTTTAAAAATGGGGA
TTGACCAAGGCAATCTGGATGACATCAAGAAAATTTACGACGAAATCACAGCCGATTCGACGAAACTAGTCAAGAATAAT
AAATTTGATTTGACTCGTTTGGCTAATATCACAGATAGTGGTGTTAAAAGTCTCATGTCAGCTAAATTTTTGCATGCTGA
AAATCAGGGCATCGAGAATAGCCTAGAAGTATCAGAAAAAATGGGAACTCCTAGCATTCCATTGATTTCCTATATCCGAA
TTTTGTCGATTCTGTTTGATAATGCCATAGAAGGAGCTTTGGAATCTGCGGAGCCAAAAATCTCAGTCGCTAACTTTTAC
CAAGATGGTGATTTTGTTTTCATGATTGAAAATTCAAGTAAGGAAAAATCAGTGGATTTAGGCAAGATTTTTGAGCGAGG
TTACTCAACCAGGGGTGAAAATCGTGGCCTTGGTCTGGCAACACTAATGGATTTCCAAGATGACTACGAAAATCTGTCGG
TTGAAACCCACAGTTCAGATTACACATTCACACAAGTGGTGAGAATATATGAGGGAGATAGTAGATGA

Domains

Predicted by InterproScan.

• GHKL domain (HATPase_c_5) (212-309)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comD/comD3	Streptococcus equinus JB1	79.167	99.048	0.784
comD/comD1	Streptococcus equinus JB1	49.784	96.25	0.479
comD/blpH	Streptococcus mutans UA159	37.785	97.46	0.368

References

• [1]: Narito Asanuma et al. (2010) Involvement of two-component signal transduction system, ComDE, in the regulation of growth and genetic transformation, in the ruminal bacterium Streptococcus bovis. Anaerobe 16(4):405-11. [PMID: 20478389]