Detailed information    

experimental Experimentally validated

Overview

Name   comE/comE2   Type   Regulator
Locus tag   H702_RS02385 Genome accession   NZ_AUZH01000012
Coordinates   12914..13648 (+) Length   244 a.a.
NCBI ID   WP_052071091.1    Uniprot ID   A0A091BRI3
Organism   Streptococcus equinus JB1     
Function   activate transcription of early competence genes   
Competence regulation

Function

The transcript level of comED was decreased by the disruption of comCD, suggesting that the transcription of comED might be stimulated by ComC.


Genomic Context

Location: 7914..18648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H702_RS10235 (H702_02440) comC/comC1 7951..8094 (-) 144 WP_160172983.1 hypothetical protein Regulator
  H702_RS02355 (H702_02445) - 8358..8507 (+) 150 WP_039696228.1 hypothetical protein -
  H702_RS02360 (H702_02450) - 8520..8825 (+) 306 WP_039696229.1 bacteriocin immunity protein -
  H702_RS02365 (H702_02455) - 8853..9146 (+) 294 WP_039696231.1 bacteriocin immunity protein -
  H702_RS02375 (H702_02465) comD/comD2 9933..10880 (+) 948 WP_160172984.1 sensor histidine kinase Regulator
  H702_RS02380 (H702_02470) comD/comD3 10877..12253 (+) 1377 WP_039696235.1 sensor histidine kinase Regulator
  H702_RS02385 (H702_02475) comE/comE2 12914..13648 (+) 735 WP_052071091.1 LytR/AlgR family response regulator transcription factor Regulator
  H702_RS02395 (H702_02485) rsgA 13966..14838 (+) 873 WP_039696236.1 ribosome small subunit-dependent GTPase A -
  H702_RS02400 (H702_02490) rpe 14845..15507 (+) 663 WP_006531895.1 ribulose-phosphate 3-epimerase -
  H702_RS02405 (H702_02495) - 15500..16132 (+) 633 WP_039696237.1 thiamine diphosphokinase -
  H702_RS02410 (H702_02500) rmuC 16134..17408 (+) 1275 WP_039696239.1 DNA recombination protein RmuC -
  H702_RS02415 (H702_02505) - 17398..18336 (+) 939 WP_039696240.1 3'-5' exoribonuclease YhaM family protein -

Sequence

Protein


Download         Length: 244 a.a.        Molecular weight: 28825.11 Da        Isoelectric Point: 6.4989

>NTDB_id=545 H702_RS02385 WP_052071091.1 12914..13648(+) (comE/comE2) [Streptococcus equinus JB1]
MIDIYVLEDNVNQQFRIEKMIGDLLETNQWETRHFDLFSEPDCLTNLVEESFPQIFFLDIDIHGDNTKGIEVAKAIREKS
RTATIVFVTTHSEFMLLTYRAQVSALDFIDKTDKDDAIKDNLKKCLQKVFEDQGFNIPKEIFVFENNKTKIRIPLEDILY
FETAESHRLKLITKTGQRHFYGSIKEVMQANQKLFRCHKSYLINLNNIVRLDKKEGLVYFDDDKACYVSRKYIKDLRSKM
ENLK

Nucleotide


Download         Length: 735 bp        

>NTDB_id=545 H702_RS02385 WP_052071091.1 12914..13648(+) (comE/comE2) [Streptococcus equinus JB1]
ATGATTGATATCTATGTATTAGAAGATAATGTAAATCAGCAGTTCCGTATTGAAAAAATGATTGGAGATTTACTCGAGAC
AAATCAGTGGGAAACTCGTCATTTTGATTTATTTAGTGAACCTGATTGTTTAACTAACCTAGTTGAGGAGTCTTTTCCTC
AAATTTTTTTCCTGGATATTGATATTCATGGAGATAATACTAAAGGAATTGAAGTGGCTAAGGCGATAAGGGAAAAATCA
CGCACAGCGACAATTGTTTTTGTGACGACTCACTCTGAATTCATGTTATTAACTTATCGAGCGCAAGTTAGTGCACTTGA
TTTTATTGATAAAACAGATAAAGATGATGCGATAAAAGATAACTTAAAAAAATGCTTGCAAAAGGTCTTTGAGGACCAAG
GTTTTAACATTCCTAAGGAAATATTTGTTTTTGAAAATAACAAAACGAAAATCCGCATTCCCTTGGAAGATATCCTTTAT
TTTGAAACCGCGGAAAGTCATCGCCTGAAGTTGATTACAAAAACTGGACAACGTCATTTTTATGGTTCAATCAAAGAAGT
GATGCAAGCTAATCAGAAATTATTTAGATGTCATAAATCATATCTGATTAACCTAAATAATATTGTTAGGTTAGATAAAA
AAGAAGGTTTGGTTTATTTTGACGATGATAAAGCCTGTTATGTTTCTAGAAAATATATCAAAGACTTAAGGTCAAAAATG
GAAAATCTGAAATAG

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(19-120)

LytTr DNA-binding domain (LytTR)

(151-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A091BRI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE/comE1 Streptococcus equinus JB1

45.041

99.588

0.449
  comE/blpR Streptococcus mutans UA159

40.664

98.77

0.402
  comE/comE2 Streptococcus gordonii strain NCTC7865

36.255

100

0.373
  comE/comE1 Streptococcus gordonii str. Challis substr. CH1

36.255

100

0.373
  comE Streptococcus pneumoniae TIGR4

35.039

100

0.365
  comE Streptococcus mitis SK321

35.039

100

0.365
  comE Streptococcus pneumoniae Rx1

35.039

100

0.365
  comE Streptococcus mitis NCTC 12261

35.039

100

0.365
  comE Streptococcus pneumoniae D39

35.039

100

0.365
  comE Streptococcus pneumoniae R6

35.039

100

0.365

Multiple sequence alignment    



References

[1] Narito Asanuma et al. (2010) Involvement of two-component signal transduction system, ComDE, in the regulation of growth and genetic transformation, in the ruminal bacterium Streptococcus bovis. Anaerobe 16(4):405-11. [PMID: 20478389]