Detailed information NTG0000420

Experimentally validated

Overview

• Name: comE/comE2
• Type: Regulator
• Locus tag: DQL23_RS10740
• Genome accession: NZ_LS483341
• Coordinates: 2182135..2182902 (-)
• Length: 255 a.a.
• NCBI ID: WP_008808031.1
• Uniprot ID: P75031
• Organism: Streptococcus gordonii strain NCTC7865
• Function: activate transcription of early competence genes Competence regulation

Function

In S. gordonii, pre-CSP is a 50 aa polypeptide that is cleaved at a double glycine to generate the mature 19 aa residue CSP. This peptide is then exported out of the cell via an ABC-binding cassette transporter encoded by comA and comB. Once a critical concentration of environmental CSP is reached, it is detected by the two-component system ComDE, comprising a membrane-bound histidine kinase (ComD), which phosphorylates the intracellular response regulator ComE. ComE activates a signalling cascade that includes upregulation of the master regulator SigX (designated ComR in S. gordonii), an alternate sigma factor that controls expression of late competence genes encoding DNA uptake and recombination machinery.

Genomic Context

Location: 2177135..2187902

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL23_RS10720 (NCTC7865_02161)	pth	2180055..2180624 (-)	570	WP_060553299.1	aminoacyl-tRNA hydrolase	-
DQL23_RS10725 (NCTC7865_02162)	ychF	2180698..2181813 (-)	1116	WP_008808030.1	redox-regulated ATPase YchF	-
DQL23_RS10730 (NCTC7865_02162)	-	2181923..2181996 (-)	74	WP_008808030.1	tRNA-Asn	-
DQL23_RS10735 (NCTC7865_02162)	-	2182002..2182073 (-)	72	WP_008808030.1	tRNA-Glu	-
DQL23_RS10740 (NCTC7865_02165)	comE/comE2	2182135..2182902 (-)	768	WP_008808031.1	competence system response regulator transcription factor ComE	Regulator
DQL23_RS10745 (NCTC7865_02166)	comD/comD2	2182899..2184257 (-)	1359	WP_060553298.1	competence system sensor histidine kinase ComD	Regulator
DQL23_RS10750 (NCTC7865_02167)	comC/comC2	2184273..2184425 (-)	153	WP_008808033.1	bacteriocin	Regulator
DQL23_RS10755 (NCTC7865_02167)	-	2184596..2184669 (-)	74	WP_008808033.1	tRNA-Arg	-
DQL23_RS10760 (NCTC7865_02169)	rlmH	2184712..2185191 (-)	480	WP_060553297.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
DQL23_RS10765 (NCTC7865_02170)	htrA	2185385..2186578 (+)	1194	WP_060553296.1	S1C family serine protease	Regulator
DQL23_RS10770 (NCTC7865_02171)	spo0J	2186644..2187408 (+)	765	WP_045772585.1	ParB/RepB/Spo0J family partition protein	Regulator

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comE/comE2	comC/comC2	positive effect
comC/comC2	comD/comD2	positive effect
comD/comD2	comE/comE2	positive effect
comE/comE2	comD/comD2	positive effect

Sequence

Protein

• Length: 255 a.a.
• Molecular weight: 30225.48 Da
• Isoelectric Point: 7.0651

>NTDB_id=420 DQL23_RS10740 WP_008808031.1 2182135..2182902(-) (comE/comE2) [Streptococcus gordonii strain NCTC7865]
MKVLVLEDTIEHQVRIENVFEEISRELNLEIKAKVTGKIHEFKEYVESDEVNQLYFLDIDIKGEEQKGLEMAQFIRQHNP
YAIIVFVTSHSEFATLTFKYKVSALDFIDKDINDNSFKKRVKDCIVYTRNNLIENTDIVDFFEYSFRGNEIRIPFRDILY
IETTGSPYKLRVVGKNFFKEFYGTIAEIQEQDKELGYFFSPHKSYLSNISNISGYDKKTKDVLYYDGHRSPISRLRVRYL
KEILKAKSKKEENKK

Nucleotide

• Length: 768 bp

>NTDB_id=420 DQL23_RS10740 WP_008808031.1 2182135..2182902(-) (comE/comE2) [Streptococcus gordonii strain NCTC7865]
ATGAAAGTATTAGTGCTAGAAGATACTATTGAACATCAAGTGAGAATTGAAAATGTCTTCGAAGAAATTTCTCGCGAACT
TAATCTTGAAATTAAAGCAAAAGTAACAGGAAAAATTCATGAATTTAAAGAATATGTTGAGTCAGACGAGGTAAATCAAC
TCTATTTCTTAGATATAGATATCAAAGGAGAAGAACAAAAAGGTCTAGAAATGGCCCAATTTATACGCCAACATAATCCC
TATGCTATCATTGTTTTTGTTACGAGTCATTCAGAATTTGCGACCTTAACTTTTAAGTATAAAGTATCAGCGTTAGATTT
TATAGACAAAGACATAAATGATAATTCTTTTAAGAAAAGAGTTAAAGATTGCATCGTTTATACACGAAATAATTTAATTG
AAAATACAGATATTGTTGACTTTTTTGAGTATAGTTTTAGAGGAAACGAAATTCGAATTCCTTTTAGAGATATTTTATAT
ATTGAGACAACAGGAAGTCCTTATAAATTAAGAGTTGTTGGAAAAAATTTCTTTAAGGAATTCTATGGAACAATAGCAGA
AATTCAGGAACAAGATAAAGAACTTGGATATTTTTTCTCACCACACAAGTCATACCTATCTAATATTAGTAATATTAGTG
GATACGACAAAAAAACAAAAGATGTTTTGTATTATGATGGTCATCGTTCACCAATTTCTCGATTACGTGTCAGATATTTA
AAAGAAATACTGAAAGCAAAAAGTAAAAAAGAAGAAAATAAAAAATAA

Domains

Predicted by InterProScan.

• LytTr DNA-binding domain (LytTR) (151-244)

• Response regulator receiver domain (Response_reg) (3-121)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE/comE1	Streptococcus gordonii str. Challis substr. CH1	100	100	1
comE	Streptococcus mitis NCTC 12261	64	100	0.64
comE	Streptococcus infantis strain Atu-4	64	99.602	0.637
comE	Streptococcus mitis SK321	63.2	100	0.632
comE	Streptococcus pneumoniae R6	62.8	100	0.628
comE	Streptococcus pneumoniae D39	62.8	100	0.628
comE	Streptococcus pneumoniae Rx1	62.8	100	0.628
comE	Streptococcus pneumoniae TIGR4	62.8	100	0.628
comE/comE2	Streptococcus equinus JB1	36.255	100	0.373
comE/blpR	Streptococcus mutans UA159	37.551	98	0.368

References

• [1]: L S Håvarstein et al. (1996) Identification of the streptococcal competence-pheromone receptor. Molecular Microbiology 21(4):863-9. [PMID: 8878047]