Detailed information NTG0000419

Experimentally validated

Overview

• Name: comD/comD2
• Type: Regulator
• Locus tag: DQL23_RS10745
• Genome accession: NZ_LS483341
• Coordinates: 2182899..2184257 (-)
• Length: 452 a.a.
• NCBI ID: WP_060553298.1
• Uniprot ID: -
• Organism: Streptococcus gordonii strain NCTC7865
• Function: phosphorylation of ComE Competence regulation

Function

In S. gordonii, pre-CSP is a 50 aa polypeptide that is cleaved at a double glycine to generate the mature 19 aa residue CSP. This peptide is then exported out of the cell via an ABC-binding cassette transporter encoded by comA and comB. Once a critical concentration of environmental CSP is reached, it is detected by the two-component system ComDE, comprising a membrane-bound histidine kinase (ComD), which phosphorylates the intracellular response regulator ComE. ComE activates a signalling cascade that includes upregulation of the master regulator SigX (designated ComR in S. gordonii), an alternate sigma factor that controls expression of late competence genes encoding DNA uptake and recombination machinery.

Genomic Context

Location: 2177899..2189257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL23_RS10720 (NCTC7865_02161)	pth	2180055..2180624 (-)	570	WP_060553299.1	aminoacyl-tRNA hydrolase	-
DQL23_RS10725 (NCTC7865_02162)	ychF	2180698..2181813 (-)	1116	WP_008808030.1	redox-regulated ATPase YchF	-
DQL23_RS10730 (NCTC7865_02162)	-	2181923..2181996 (-)	74	WP_008808030.1	tRNA-Asn	-
DQL23_RS10735 (NCTC7865_02162)	-	2182002..2182073 (-)	72	WP_008808030.1	tRNA-Glu	-
DQL23_RS10740 (NCTC7865_02165)	comE/comE2	2182135..2182902 (-)	768	WP_008808031.1	competence system response regulator transcription factor ComE	Regulator
DQL23_RS10745 (NCTC7865_02166)	comD/comD2	2182899..2184257 (-)	1359	WP_060553298.1	competence system sensor histidine kinase ComD	Regulator
DQL23_RS10750 (NCTC7865_02167)	comC/comC2	2184273..2184425 (-)	153	WP_008808033.1	bacteriocin	Regulator
DQL23_RS10755 (NCTC7865_02167)	-	2184596..2184669 (-)	74	WP_008808033.1	tRNA-Arg	-
DQL23_RS10760 (NCTC7865_02169)	rlmH	2184712..2185191 (-)	480	WP_060553297.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
DQL23_RS10765 (NCTC7865_02170)	htrA	2185385..2186578 (+)	1194	WP_060553296.1	S1C family serine protease	Regulator
DQL23_RS10770 (NCTC7865_02171)	spo0J	2186644..2187408 (+)	765	WP_045772585.1	ParB/RepB/Spo0J family partition protein	Regulator

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comD/comD2	comE/comE2	positive effect
comE/comE2	comC/comC2	positive effect
comC/comC2	comD/comD2	positive effect
comE/comE2	comD/comD2	positive effect

Sequence

Protein

• Length: 452 a.a.
• Molecular weight: 52956.68 Da
• Isoelectric Point: 6.0733

>NTDB_id=419 DQL23_RS10745 WP_060553298.1 2182899..2184257(-) (comD/comD2) [Streptococcus gordonii strain NCTC7865]
MILKIIIAFFPTIIQVVSYEIFHKKIMQSKKIDIKFIGLFFSIVFGISIISSILIEIPSFGDVISSIFHYIFLFIQPLIF
YKYFLKRKEYDNYLNLFLSFVIYLSVETSETFLSVIISSITGDYFVKQHYDIFYIIINLLSLFIILKVVDFFDFYFEYYK
EPIYKNDLYNVNKSYIVIHILLNISHWFSENAHLNSFASMIATIGFIMFLSTLFYLKSAREQHEKAKEIQQKKEEQRQLQ
LYTDEIVGLYNEIRGFRHDYAGMLISLQTGINSGDMKEVERIFHNVLSQANISLRSDDYTFFELNNVQDTALRSVLIQTI
FKARECGVEIVFEMKDVIETLPMKLLDLVRVASVLLNNAVEGAAESPSKTMNVSLVKLDKEIVFVIQNSRQSRYINLEEI
YEVGFSTKGENRGLGLNNVKEIIDKYDEVILETDIETNYFIQVVRFKRKEKI

Nucleotide

• Length: 1359 bp

>NTDB_id=419 DQL23_RS10745 WP_060553298.1 2182899..2184257(-) (comD/comD2) [Streptococcus gordonii strain NCTC7865]
ATGATTTTGAAGATAATAATTGCATTTTTTCCAACTATTATACAAGTTGTTTCTTATGAGATATTTCATAAGAAAATTAT
GCAGAGTAAAAAGATAGATATAAAATTTATTGGTTTATTCTTTAGTATTGTTTTTGGAATTTCAATTATTTCTTCAATTC
TTATTGAAATTCCAAGTTTTGGAGATGTAATAAGTAGTATTTTCCACTATATTTTCCTATTTATTCAACCCCTTATTTTC
TACAAATATTTTCTTAAAAGAAAAGAGTATGATAATTACTTGAATTTGTTTTTATCGTTTGTTATCTATTTATCTGTGGA
GACTTCCGAAACTTTTTTATCTGTCATTATTTCTTCAATAACAGGAGATTATTTTGTAAAACAACATTACGATATTTTTT
ACATTATAATTAATCTTTTATCTTTGTTTATTATTTTAAAGGTTGTTGATTTTTTTGACTTTTATTTTGAATATTATAAA
GAACCAATTTATAAAAATGATCTATATAATGTTAATAAGTCATATATAGTAATTCATATCTTATTAAATATTTCTCATTG
GTTTAGCGAAAACGCACATCTTAATAGTTTTGCTAGTATGATTGCCACTATAGGATTTATTATGTTTTTGTCGACTTTAT
TTTATTTAAAGTCTGCTCGTGAACAACATGAAAAAGCAAAAGAAATTCAGCAAAAAAAGGAAGAACAAAGACAACTACAG
CTTTATACAGATGAAATTGTAGGTCTTTATAACGAAATTCGAGGTTTTCGTCATGATTATGCTGGTATGTTAATAAGTTT
ACAAACAGGAATTAACTCAGGTGATATGAAAGAAGTAGAGAGAATCTTTCATAATGTTTTAAGTCAAGCTAATATTTCCC
TTCGATCAGATGACTATACTTTTTTTGAGTTAAATAATGTTCAAGACACTGCCTTGAGAAGTGTGCTAATTCAAACTATA
TTTAAGGCTCGAGAGTGTGGAGTAGAGATCGTATTTGAAATGAAGGATGTTATTGAGACATTACCTATGAAACTTTTAGA
TTTAGTTCGAGTAGCTAGTGTATTGCTCAATAATGCGGTTGAAGGGGCAGCTGAAAGTCCCTCAAAAACAATGAACGTGT
CTTTAGTAAAGCTAGATAAAGAAATTGTTTTTGTAATTCAGAATTCTCGTCAAAGCCGTTATATTAATTTAGAAGAAATT
TATGAAGTAGGCTTCTCAACAAAAGGAGAAAATCGAGGATTAGGTTTAAATAATGTCAAAGAAATTATTGACAAATATGA
TGAAGTTATTCTGGAAACAGATATAGAAACAAATTATTTTATACAAGTTGTAAGATTTAAAAGAAAGGAAAAAATATGA

Domains

Predicted by InterProScan.

• GHKL domain (HATPase_c_5) (343-444)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comD/comD1	Streptococcus gordonii str. Challis substr. CH1	89.845	100	0.9
comD/comD1	Streptococcus pneumoniae Rx1	47.42	90.044	0.427
comD/comD1	Streptococcus pneumoniae D39	47.42	90.044	0.427
comD/comD1	Streptococcus pneumoniae R6	47.42	90.044	0.427
comD/comD2	Streptococcus pneumoniae TIGR4	49.468	85.261	0.422
comD	Streptococcus mitis SK321	47.132	88.717	0.418
comD	Streptococcus mitis NCTC 12261	47.044	88.209	0.415

References

• [1]: L S Håvarstein et al. (1996) Identification of the streptococcal competence-pheromone receptor. Molecular Microbiology 21(4):863-9. [PMID: 8878047]