Detailed information NTG0000515

Experimentally validated

Overview

• Name: comE
• Type: Regulator
• Locus tag: SMSK321_RS0102895
• Genome accession: NZ_AEDT01000032
• Coordinates: 233174..233926 (-)
• Length: 250 a.a.
• NCBI ID: WP_000866074.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: activate transcription of early competence genes Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 228174..238926

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS10605 (SMSK321_1632)	-	229619..232261 (-)	2643	WP_000472077.1	YhgE/Pip domain-containing protein	-
SMSK321_RS10610 (SMSK321_1633)	-	232389..232931 (+)	543	WP_001158273.1	TetR/AcrR family transcriptional regulator	-
SMSK321_RS0102895	comE	233174..233926 (-)	753	WP_000866074.1	competence system response regulator transcription factor ComE	Regulator
SMSK321_RS10615 (SMSK321_1634)	comD	233923..235248 (-)	1326	WP_001048129.1	competence system sensor histidine kinase ComD	Regulator
SMSK321_RS0102900	comC	235269..235394 (-)	126	WP_000799685.1	competence-stimulating peptide ComC	Regulator
SMSK321_RS10620 (SMSK321_1635)	rlmH	235677..236156 (-)	480	WP_000695928.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
SMSK321_RS10625 (SMSK321_1636)	htrA	236340..237521 (+)	1182	WP_000681589.1	S1C family serine protease	Regulator
SMSK321_RS10630 (SMSK321_1637)	spo0J	237579..238337 (+)	759	WP_000410371.1	ParB/RepB/Spo0J family partition protein	Regulator

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comE	comE	positive effect
comE	comM	positive effect
comE	comX/sigX/comX1/sigX1	positive effect
comE	comC	positive effect
comE	comD	positive effect
comE	comA	positive effect
comE	comX/sigX/comX2/sigX2	positive effect
comE	comW	positive effect
comE	comB	positive effect
comD	comE	positive effect
comM	cbpD	negative effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comW	comX/sigX/comX1/sigX1	positive effect
comC	comD	positive effect
comB	comC	positive effect
comA	comC	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comW	comX/sigX/comX2/sigX2	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect

Sequence

Protein

• Length: 250 a.a.
• Molecular weight: 29939.34 Da
• Isoelectric Point: 6.9221

>NTDB_id=515 SMSK321_RS0102895 WP_000866074.1 233174..233926(-) (comE) [Streptococcus mitis SK321]
MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFKEYIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNP
YAIIVFITSRSEFATLTYKYQVSALDFVDKDINDELFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKNLEIVFYEDHRCPISRLKIRKL
KDILEKKSQK

Nucleotide

• Length: 753 bp

>NTDB_id=515 SMSK321_RS0102895 WP_000866074.1 233174..233926(-) (comE) [Streptococcus mitis SK321]
ATGAAAGTTTTAATTTTAGAAGATGTTATTGAACATCAAGTGAGACTAGAGAGAATATTGGATGAAATTTCGAAAGAATC
GAATATTCCAATATCATACAAGACAACGGGAAAAGTTCGTGAGTTTAAGGAATATATCGAAAATGATGAAGTAAACCAGC
TTTATTTTCTAGATATTGATATTCATGGAATTGAGAAAAAGGGATTTGAAGTGGCTCAGCTCATTCGTCATTACAATCCT
TACGCTATTATCGTCTTTATCACCAGTCGATCAGAGTTTGCGACTCTAACATATAAATACCAGGTATCAGCCCTAGATTT
TGTTGATAAGGATATCAATGATGAGTTATTTAAGAAGAGAATTGAGCAAAATATCTTCTACACGAAGAGTATGTTACTTG
AAAATGAAGATGTGGTAGATTATTTTGACTACAATTACAAGGGAAATGATTTAAAAATTCCTTATCATGACATTTTGTAT
ATTGAAACGACAGGAGTTTCTCATAAATTGCGCATTATTGGTAAGAATTTTGCAAAAGAATTTTATGGTACCATGACAGA
TATTCAGGAAAAGGACAAACATACTCAGCGATTTTATTCTCCTCATAAGTCATTTTTGGTAAATATAGGTAATATTAGAG
AAATTGATCGAAAAAACCTAGAAATTGTTTTCTATGAAGATCATCGTTGTCCTATTTCAAGATTAAAAATTAGAAAATTG
AAAGATATTCTAGAGAAAAAATCTCAAAAGTAA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (3-121)

• LytTr DNA-binding domain (LytTR) (151-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Streptococcus mitis NCTC 12261	99.2	100	0.992
comE	Streptococcus pneumoniae Rx1	99.2	100	0.992
comE	Streptococcus pneumoniae D39	99.2	100	0.992
comE	Streptococcus pneumoniae R6	99.2	100	0.992
comE	Streptococcus pneumoniae TIGR4	99.2	100	0.992
comE	Streptococcus infantis strain Atu-4	92	100	0.92
comE/comE1	Streptococcus gordonii str. Challis substr. CH1	63.2	100	0.632
comE/comE2	Streptococcus gordonii strain NCTC7865	63.2	100	0.632
comE/blpR	Streptococcus mutans UA159	41.296	98.8	0.408
comE/comE2	Streptococcus equinus JB1	35.039	100	0.365

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]