Detailed information NTG0000511

Experimentally validated

Overview

• Name: comA
• Type: Regulator
• Locus tag: SMSK321_RS10730
• Genome accession: NZ_AEDT01000033
• Coordinates: 3327..5480 (+)
• Length: 717 a.a.
• NCBI ID: WP_000668322.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: processing and transport of ComC Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 1..10480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS10705 (SMSK321_1304)	-	85..816 (+)	732	WP_001221870.1	response regulator transcription factor	-
SMSK321_RS10710 (SMSK321_1305)	-	1506..1703 (+)	198	WP_000180825.1	class IIb bacteriocin, lactobin A/cerein 7B family	-
SMSK321_RS10715 (SMSK321_1306)	-	1917..2072 (+)	156	WP_000357248.1	smi_0061 family bacteriocin-like peptide	-
SMSK321_RS12550	-	2076..2291 (+)	216	WP_080550793.1	bacteriocin immunity protein	-
SMSK321_RS10720 (SMSK321_1308)	-	2372..2551 (+)	180	WP_000846993.1	hypothetical protein	-
SMSK321_RS12555 (SMSK321_1309)	-	2980..3168 (+)	189	WP_000757184.1	hypothetical protein	-
SMSK321_RS10730 (SMSK321_1310)	comA	3327..5480 (+)	2154	WP_000668322.1	peptide cleavage/export ABC transporter ComA	Regulator
SMSK321_RS10735 (SMSK321_1311)	comB	5493..6842 (+)	1350	WP_000801634.1	competence pheromone export protein ComB	Regulator
SMSK321_RS10740 (SMSK321_1312)	purC	7012..7719 (+)	708	WP_000043295.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
SMSK321_RS12650	-	7721..7864 (+)	144	WP_079211609.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comA	comC	positive effect
comC	comD	positive effect
comD	comE	positive effect
comE	comE	positive effect
comE	comM	positive effect
comE	comX/sigX/comX1/sigX1	positive effect
comE	comC	positive effect
comE	comD	positive effect
comE	comA	positive effect
comE	comX/sigX/comX2/sigX2	positive effect
comE	comW	positive effect
comE	comB	positive effect
comM	cbpD	negative effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comW	comX/sigX/comX1/sigX1	positive effect
comB	comC	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comW	comX/sigX/comX2/sigX2	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect

Sequence

Protein

• Length: 717 a.a.
• Molecular weight: 80275.37 Da
• Isoelectric Point: 6.6494

>NTDB_id=511 SMSK321_RS10730 WP_000668322.1 3327..5480(+) (comA) [Streptococcus mitis SK321]
MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYSLAHLRELAKTTMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDL
TFPFVAHVLKEGKLLHYYVVTGQDKGSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKDQKNGLLSFIPI
LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQILSYAQEYLLLVLGQRLSID
VILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSIIDALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTV
IIFAFMKPFEKMNRDTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQKALKKVAHL
LLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRLNEVYLVTSEFEEKKTVEDLSLM
KGEMTFKQVYYKYGYGRDVLSDINLTIPQGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYI
NYLPQQPYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARALLTDA
PILILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEKVVVLNQGKIVEEGKHADLLAQGGFYAHLVNS

Nucleotide

• Length: 2154 bp

>NTDB_id=511 SMSK321_RS10730 WP_000668322.1 3327..5480(+) (comA) [Streptococcus mitis SK321]
ATGAAATTTGGGAAACGTCACTATCGTCCGCAAGTGGATCAGATGGACTGCGGTGTAGCTTCATTAGCCATGGTTTTTGG
ATACTATGGTAGTTATTATTCTTTGGCTCACTTGCGAGAATTGGCTAAGACGACCATGGATGGGACGACGGCCTTGGGCT
TGGTCAAGGTGGCAGAGGAGATTGGTTTTGAGACGCGAGCAATTAAGGCGGATATGACGCTTTTTGACTTGCCAGATTTG
ACCTTTCCTTTTGTTGCCCATGTGCTTAAGGAAGGAAAATTGCTCCACTACTATGTGGTGACTGGGCAGGATAAGGGTAG
CATTCATATTGCCGACCCAGATCCTGGGGTGAAATTGACCAAACTGCCACGTGAGCGTTTTGCGGAAGAATGGACAGGAG
TGACTCTGTTTATGGCACCTAGTCCAGACTACAAGCCTCATAAGGATCAAAAGAATGGTCTGCTCTCTTTTATCCCTATA
TTAGTGAAGCAGCGTGGCTTGATCGCTAATATCGTTTTGGCAACACTCTTGGTAACCGTGATTAACATTGTGGGTTCTTA
TTATCTGCAGTCTATCATTGATACCTATGTGCCAGATCAGATGCGTTCGACGCTTGGGATTATTTCTATTGGGCTGGTCA
TCGTCTATATCCTCCAGCAGATCTTGTCTTACGCTCAGGAGTATCTCTTGCTTGTTTTGGGGCAACGCTTGTCGATTGAT
GTGATTTTGTCCTATATCAAGCATGTTTTTCACCTGCCTATGTCCTTCTTTGCGACACGCAGGACAGGGGAAATCGTGTC
TCGTTTCACGGATGCTAACAGTATCATTGATGCGCTGGCTTCGACCATTCTTTCGATTTTCCTAGATGTGTCAACGGTTG
TCATTATTTCTCTTGTTTTATTTTCACAAAATACCAATCTCTTTTTCATGACCTTATTGGCACTTCCTATCTATACAGTG
ATTATCTTTGCCTTTATGAAGCCGTTTGAAAAGATGAATCGGGATACCATGGAAGCCAATGCGGTTCTGTCTTCTTCTAT
CATTGAGGACATCAACGGTATTGAGACTATCAAGTCTTTGACCAGTGAAAGTCAACGTTACCAAAAGATTGACAAGGAAT
TTGTAGATTATCTGAAAAAATCCTTTACCTATAGTCGGGCAGAGAGTCAGCAAAAGGCTCTGAAAAAAGTTGCCCATCTC
TTGCTCAATGTCGGCATTCTCTGGATGGGGGCTGTTCTGGTTATGGATGGCAAGATGAGTTTGGGGCAGTTGATTACCTA
TAATACCTTGCTGGTTTACTTTACCAATCCTTTGGAAAATATCATCAACCTGCAAACCAAACTTCAGACAGCGCAGGTCG
CCAATAACCGTCTAAATGAGGTTTATCTAGTAACTTCGGAGTTTGAGGAGAAGAAAACAGTTGAGGATTTGAGCTTGATG
AAGGGAGAGATGACTTTCAAGCAGGTTTACTATAAGTATGGCTATGGTCGAGATGTCTTGTCAGATATCAATTTGACCAT
TCCCCAAGGGTCTAAGGTGGCTTTTGTGGGAATTTCGGGGTCAGGTAAGACGACATTGGCCAAGATGATGGTTAATTTTT
ATGATCCAAGTCAGGGAGAGATTAGTCTGGGTGGTGTCAATCTCAATCAGATCGATAAAAAGGCCTTGCGCCAGTACATC
AACTACCTGCCTCAACAGCCCTATGTCTTTAACGGAACGATTTTGGAGAATCTTCTTTTGGGAGCCAAGGAGGGGACGAC
TCAAGAGGATATCTTACGGGCGGTCGAATTGGCAGAGATTCGAGAGGACATTGAGCGTATGCCACTGAATTACCAGACAG
AATTGACTTCGGATGGGGCAGGAATCTCAGGTGGTCAACGTCAGAGAATTGCTCTGGCGCGTGCTCTCTTAACAGATGCA
CCGATCTTGATTTTGGACGAGGCGACCAGCAGTCTGGATATCTTGACTGAGAAGCGAATTGTCGATAATCTTATGGCTTT
AGACAAGACCTTGATTTTCATTGCCCACCGTTTGACTATTGCTGAGCGGACAGAGAAGGTGGTTGTCTTGAATCAGGGCA
AGATTGTTGAAGAAGGAAAGCATGCTGATTTGCTTGCACAGGGTGGTTTTTACGCCCATTTGGTCAATAGCTAG

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (500-650)

• ABC transporter transmembrane region (ABC_membrane) (170-438)

• Peptidase C39 family (Peptidase_C39) (7-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Streptococcus pneumoniae Rx1	98.466	100	0.985
comA	Streptococcus pneumoniae R6	98.466	100	0.985
comA	Streptococcus pneumoniae D39	98.466	100	0.985
comA	Streptococcus mitis NCTC 12261	98.466	100	0.985
comA	Streptococcus pneumoniae TIGR4	98.047	100	0.98
comA	Streptococcus gordonii str. Challis substr. CH1	80.335	100	0.803
comA/nlmT	Streptococcus mutans UA159	64.714	100	0.647

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]