Detailed information NTG0000358

Experimentally validated

Overview

• Name: comA/nlmT
• Type: Regulator
• Locus tag: SMU_RS01425
• Genome accession: NC_004350
• Coordinates: 270628..272910 (+)
• Length: 760 a.a.
• NCBI ID: WP_002263523.1
• Uniprot ID: Q8DW05
• Organism: Streptococcus mutans UA159
• Function: transport of ComC Competence regulation

Function

CSP and the mutacins are exported outside the cell by the same ABC transporter, NlmTE.

Genomic Context

Location: 265628..277910

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS01390 (SMU.277)	-	266388..266663 (+)	276	WP_025983783.1	hypothetical protein	-
SMU_RS01395 (SMU.278)	-	266877..267038 (+)	162	WP_162470319.1	hypothetical protein	-
SMU_RS01400 (SMU.279)	-	267523..267732 (+)	210	WP_002263944.1	hypothetical protein	-
SMU_RS01405 (SMU.281)	-	267970..268248 (+)	279	WP_002264456.1	hypothetical protein	-
SMU_RS01410	-	268857..269042 (+)	186	WP_002309971.1	hypothetical protein	-
SMU_RS01415 (SMU.283)	-	269323..269541 (+)	219	WP_002278706.1	Blp family class II bacteriocin	-
SMU_RS01420 (SMU.284)	-	269566..269973 (+)	408	WP_002263726.1	hypothetical protein	-
SMU_RS01425 (SMU.286)	comA/nlmT	270628..272910 (+)	2283	WP_002263523.1	peptide cleavage/export ABC transporter	Regulator
SMU_RS01430 (SMU.287)	comB/nlmE	272924..273826 (+)	903	WP_002263524.1	HlyD family efflux transporter periplasmic adaptor subunit	Regulator
SMU_RS01435 (SMU.289)	-	274183..275850 (+)	1668	WP_002263525.1	BglG family transcription antiterminator	-
SMU_RS01440 (SMU.290)	ulaG	276429..277520 (+)	1092	WP_002263526.1	L-ascorbate 6-phosphate lactonase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comA/nlmT	comC/blpC	positive effect
comC/blpC	comX/sigX	positive effect
comX/sigX	late competence genes	positive effect
comX/sigX	late competence genes	positive effect
scnR	comX/sigX	positive effect
hdrR	comX/sigX	positive effect
mecA	comX/sigX	negative effect
clpC	comX/sigX	negative effect
comR	comX/sigX	positive effect
clpP	comX/sigX	negative effect
XrpA	comX/sigX	negative effect
scnK	comX/sigX	positive effect
comS	comX/sigX	positive effect
comD/blpH	comX/sigX	positive effect
vicK	comX/sigX	negative effect
comE/blpR	comX/sigX	positive effect
scnC	comX/sigX	positive effect
brsR	comX/sigX	positive effect
cipB	comX/sigX	positive effect
vicR	comX/sigX	negative effect
hdrR	comR	positive effect
comR	comS	positive effect
cipB	comR	positive effect
XrpA	comR	negative effect
hdrM	hdrR	negative effect
comS	comS	positive effect
oppD	comS	positive effect
cipB	comS	positive effect
XrpA	comS	negative effect
comD/blpH	comE/blpR	positive effect
comE/blpR	comD/blpH	positive effect
comE/blpR	comC/blpC	positive effect
comE/blpR	comA/nlmT	positive effect
comE/blpR	comE/blpR	positive effect
comE/blpR	comB/nlmE	positive effect
vicR	comE/blpR	negative effect
vicK	comE/blpR	negative effect
vicR	comD/blpH	negative effect
vicR	comC/blpC	negative effect
vicK	comD/blpH	negative effect
vicK	comC/blpC	negative effect
comC/blpC	comD/blpH	positive effect
comB/nlmE	comC/blpC	positive effect
ciaH	comC/blpC	positive effect
sepM	comC/blpC	positive effect
brsM	brsR	negative effect

Sequence

Protein

• Length: 760 a.a.
• Molecular weight: 85096.79 Da
• Isoelectric Point: 8.9095

>NTDB_id=358 SMU_RS01425 WP_002263523.1 270628..272910(+) (comA/nlmT) [Streptococcus mutans UA159]
MKQVIYVVLIVIAVNILLEIIKRVTKRGGTVSSSNPLPDGQSKLFWRRHYKLVPQIDTRDCGPAVLASVAKHYGSNYSIA
YLRELSKTNKQGTTALGIVEAAKKLGFETRSIKADMTLFDYNDLTYPFIVHVIKGKRLQHYYVVYGSQNNQLIIGDPDPS
VKVTRMSKERFQSEWTGLAIFLAPQPNYKPHKGEKNGLSNFFPLIFKQKALMTYIIIASLIVTLIDIVGSYYLQGILDEY
IPDQLISTLGMITIGLIITYIIQQVMAFAKEYLLAVLSLRLVIDVILSYIKHIFTLPMSFFATRRTGEITSRFTDANQII
DAVASTIFSIFLDMTMVILVGGVLLAQNNNLFFLTLLSIPIYAIIIFAFLKPFEKMNHEVMESNAVVSSSIIEDINGMET
IKSLTSESARYQNIDSEFVDYLEKNFKLHKYSAIQTALKSGAKLILNVVILWYGSRLVMDNKISVGQLITFNALLSYFSN
PIENIINLQSKLQSARVANTRLNEVYLVESEFEKDGDLSENSFLDGDISFENLSYKYGFGRDTLSDINLSIKKGSKVSLV
GASGSGKTTLAKLIVNFYEPNKGIVRINGNDLKVIDKTALRRHISYLPQQAYVFSGSIMDNLVLGAKEGTSQEDIIRACE
IAEIRSDIEQMPQGYQTELSDGAGISGGQKQRIALARALLTQAPVLILDEATSSLDILTEKKIISNLLQMTEKTIIFVAH
RLSISQRTDEVIVMDQGKIVEQGTHKELLAKQGFYYNLFN

Nucleotide

• Length: 2283 bp

>NTDB_id=358 SMU_RS01425 WP_002263523.1 270628..272910(+) (comA/nlmT) [Streptococcus mutans UA159]
ATGAAACAAGTTATTTATGTTGTTTTAATCGTCATAGCCGTTAACATTCTCTTAGAGATTATCAAAAGAGTAACAAAAAG
GGGAGGGACAGTTTCGTCATCTAATCCTTTACCAGATGGGCAGTCTAAGTTGTTTTGGCGCAGACATTATAAGCTAGTAC
CTCAGATTGATACCAGAGACTGTGGGCCGGCAGTGCTGGCATCTGTTGCAAAGCATTACGGATCTAATTACTCTATCGCT
TATCTGCGGGAACTCTCAAAGACTAACAAGCAGGGAACAACAGCTCTTGGCATTGTTGAAGCTGCTAAAAAGTTAGGCTT
TGAAACACGCTCTATCAAGGCGGATATGACGCTTTTTGATTATAATGATTTGACCTATCCTTTTATCGTCCATGTGATTA
AAGGAAAACGTCTGCAGCATTATTATGTCGTCTATGGCAGCCAGAATAATCAGCTGATTATTGGAGATCCTGATCCTTCA
GTTAAGGTGACTAGGATGAGTAAGGAACGCTTTCAATCAGAGTGGACAGGCCTTGCAATTTTCCTAGCTCCTCAGCCTAA
CTATAAGCCTCATAAAGGTGAAAAAAATGGTTTGTCTAATTTCTTCCCGTTGATCTTTAAGCAGAAAGCTTTGATGACTT
ATATTATCATAGCTAGCTTGATTGTGACGCTCATTGATATTGTCGGATCATACTATCTCCAAGGAATATTGGACGAGTAC
ATTCCTGATCAGCTGATTTCAACTTTAGGAATGATTACGATTGGTCTGATAATAACCTATATTATCCAGCAGGTCATGGC
TTTTGCAAAAGAATACCTCTTGGCCGTACTCAGTTTGCGTTTAGTCATTGATGTTATCCTGTCTTATATCAAACATATTT
TTACGCTTCCTATGTCTTTCTTTGCGACAAGGCGAACAGGAGAAATCACGTCTCGTTTTACAGATGCCAATCAGATTATT
GATGCTGTAGCGTCAACCATCTTTTCAATCTTTTTAGATATGACTATGGTAATTTTGGTTGGTGGGGTTTTGTTGGCGCA
AAACAATAACCTTTTCTTTCTAACCTTGCTCTCCATTCCGATTTATGCCATCATTATTTTTGCTTTCTTGAAACCCTTTG
AGAAAATGAATCACGAAGTGATGGAAAGCAATGCTGTGGTAAGTTCTTCTATCATTGAAGATATCAATGGGATGGAAACC
ATTAAATCACTCACAAGTGAGTCCGCTCGTTATCAAAACATTGATAGTGAATTTGTTGATTATTTGGAGAAAAACTTTAA
GCTACACAAGTATAGTGCCATTCAAACCGCATTAAAAAGCGGTGCTAAGCTTATCCTCAATGTTGTCATTCTCTGGTATG
GCTCTCGTCTAGTTATGGATAATAAAATCTCAGTTGGTCAGCTTATCACCTTTAATGCTTTGCTGTCTTATTTCTCAAAT
CCAATTGAAAATATTATCAATCTGCAATCCAAACTGCAGTCAGCTCGCGTTGCCAATACACGTCTTAATGAGGTCTATCT
TGTCGAATCTGAATTTGAAAAAGACGGCGATTTATCAGAAAATAGCTTTTTAGATGGTGATATTTCGTTTGAAAATCTTT
CTTATAAATATGGATTTGGGCGAGATACCTTATCAGATATTAATTTATCAATCAAAAAAGGCTCCAAGGTCAGTCTAGTT
GGAGCCAGTGGTTCTGGTAAAACAACTTTGGCTAAACTGATTGTCAATTTCTACGAGCCTAACAAGGGGATTGTTCGAAT
CAATGGCAATGATTTAAAAGTTATTGATAAGACAGCTTTGCGGCGGCATATTAGCTATTTGCCGCAACAGGCCTATGTTT
TTAGTGGCTCTATTATGGATAATCTCGTTTTAGGAGCTAAAGAAGGAACGAGTCAGGAAGACATTATTCGTGCTTGTGAA
ATTGCTGAAATCCGCTCGGACATTGAACAAATGCCTCAGGGCTATCAGACAGAGTTATCAGATGGTGCCGGTATTTCTGG
CGGTCAAAAACAGCGGATTGCTTTAGCTAGGGCCTTATTAACACAGGCACCGGTTTTGATTCTGGATGAAGCCACCAGCA
GTCTTGATATTTTGACAGAAAAGAAAATTATCAGCAATCTCTTACAGATGACGGAGAAAACAATAATTTTTGTTGCCCAC
CGCTTAAGCATTTCACAGCGTACTGACGAAGTCATTGTCATGGATCAGGGAAAAATTGTTGAACAAGGCACTCATAAGGA
ACTTTTAGCTAAGCAAGGTTTCTATTATAACCTGTTTAATTGA

Domains

Predicted by InterproScan.

• Peptidase C39 family (Peptidase_C39) (50-186)

• ABC transporter (ABC_tran) (544-693)

• ABC transporter transmembrane region (ABC_membrane) (212-481)

3D structure

• PDB: 3K8U
• PDB: 3VX4
• PDB: 5XE8
• PDB: 5XE9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Streptococcus mitis NCTC 12261	65.411	100	0.654
comA	Streptococcus pneumoniae R6	64.854	100	0.649
comA	Streptococcus pneumoniae Rx1	64.854	100	0.649
comA	Streptococcus pneumoniae D39	64.854	100	0.649
comA	Streptococcus mitis SK321	64.714	100	0.647
comA	Streptococcus pneumoniae TIGR4	64.575	100	0.646
comA	Streptococcus gordonii str. Challis substr. CH1	64.435	100	0.644

References

• [1]: Mohammad Shahnoor Hossain et al. (2012) An extracelluar protease, SepM, generates functional competence-stimulating peptide in Streptococcus mutans UA159. Journal of Bacteriology 194(21):5886-96. [PMID: 22923597]
• [2]: John D F Hale et al. (2005) Identification of nlmTE, the locus encoding the ABC transport system required for export of nonlantibiotic mutacins in Streptococcus mutans. Journal of Bacteriology 187(14):5036-9. [PMID: 15995224]