Detailed information NTG0000376

Experimentally validated

Overview

• Name: clpP
• Type: Regulator
• Locus tag: SMU_RS07595
• Genome accession: NC_004350
• Coordinates: 1587236..1587850 (-)
• Length: 204 a.a.
• NCBI ID: WP_255262720.1
• Uniprot ID: -
• Organism: Streptococcus mutans UA159
• Function: degradation of ComX; require for competence development (at the early growth phase); repress competence development (at the mid-logarithmic growth phase) Competence regulation

Function

MecA/ClpCP prevents adventitious entry into competence by sequestering or intercepting low levels of ComX.

Genomic Context

Location: 1582236..1592850

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS07565 (SMU.1666)	-	1582473..1583237 (-)	765	WP_002262678.1	ABC transporter ATP-binding protein	-
SMU_RS07570 (SMU.1667)	-	1583237..1584193 (-)	957	WP_002262677.1	branched-chain amino acid ABC transporter permease	-
SMU_RS07575 (SMU.1668)	-	1584197..1585066 (-)	870	WP_002262676.1	branched-chain amino acid ABC transporter permease	-
SMU_RS07580 (SMU.1669)	-	1585150..1586322 (-)	1173	WP_002262675.1	ABC transporter substrate-binding protein	-
SMU_RS07585 (SMU.1670c)	-	1586425..1586673 (-)	249	WP_002262674.1	YlbG family protein	-
SMU_RS07590 (SMU.1671c)	-	1586680..1587120 (-)	441	WP_002262673.1	YlbF family regulator	-
SMU_RS07595 (SMU.1672)	clpP	1587236..1587850 (-)	615	WP_255262720.1	ATP-dependent Clp protease proteolytic subunit ClpP	Regulator
SMU_RS07600 (SMU.1673)	upp	1588358..1588987 (-)	630	WP_002262598.1	uracil phosphoribosyltransferase	-
SMU_RS07605 (SMU.1674)	-	1589300..1590463 (-)	1164	WP_002352351.1	MalY/PatB family protein	-
SMU_RS07610 (SMU.1675)	-	1590478..1591572 (-)	1095	WP_002262596.1	cystathionine gamma-synthase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
clpP	comX/sigX	negative effect
comX/sigX	late competence genes	positive effect
comX/sigX	late competence genes	positive effect
scnR	comX/sigX	positive effect
hdrR	comX/sigX	positive effect
hdrR	comR	positive effect
hdrM	hdrR	negative effect
mecA	comX/sigX	negative effect
clpC	comX/sigX	negative effect
comR	comX/sigX	positive effect
comR	comS	positive effect
cipB	comR	positive effect
XrpA	comR	negative effect
XrpA	comX/sigX	negative effect
XrpA	comS	negative effect
scnK	comX/sigX	positive effect
comS	comX/sigX	positive effect
comS	comS	positive effect
oppD	comS	positive effect
cipB	comS	positive effect
comD/blpH	comX/sigX	positive effect
comD/blpH	comE/blpR	positive effect
vicR	comD/blpH	negative effect
comE/blpR	comD/blpH	positive effect
vicK	comD/blpH	negative effect
comC/blpC	comD/blpH	positive effect
vicK	comX/sigX	negative effect
vicK	comC/blpC	negative effect
vicK	comE/blpR	negative effect
comE/blpR	comX/sigX	positive effect
comE/blpR	comC/blpC	positive effect
comE/blpR	comA/nlmT	positive effect
comE/blpR	comE/blpR	positive effect
comE/blpR	comB/nlmE	positive effect
vicR	comE/blpR	negative effect
scnC	comX/sigX	positive effect
brsR	comX/sigX	positive effect
brsM	brsR	negative effect
cipB	comX/sigX	positive effect
vicR	comX/sigX	negative effect
vicR	comC/blpC	negative effect
comC/blpC	comX/sigX	positive effect
comA/nlmT	comC/blpC	positive effect
comB/nlmE	comC/blpC	positive effect
ciaH	comC/blpC	positive effect
sepM	comC/blpC	positive effect

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22679.13 Da
• Isoelectric Point: 5.3673

>NTDB_id=376 SMU_RS07595 WP_255262720.1 1587236..1587850(-) (clpP) [Streptococcus mutans UA159]
MELKEKYSMIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSIIAQLLFLDAQDNTKDIYLYINSPGGSVSAG
LAIVDTMNFIKSDVQTIVMGIAASMGTIIASSGAKGKRFMLPNAEYLIHQPMGGTGGGTQQSDMAIAAEQLLKTRKKLEK
ILSDNSGKTIKQIHKDAERDYWMDAKETLKYGFIDEIMENNELK

Nucleotide

• Length: 615 bp

>NTDB_id=376 SMU_RS07595 WP_255262720.1 1587236..1587850(-) (clpP) [Streptococcus mutans UA159]
ATTGAATTAAAGGAGAAATACAGTATGATTCCTGTAGTTATTGAACAAACGAGCCGTGGTGAACGCTCTTATGATATTTA
TTCACGTCTATTAAAAGATCGTATTATTATGTTGACTGGTCCTGTTGAAGACAATATGGCAAATTCCATTATTGCTCAGT
TACTGTTTCTTGATGCACAAGATAATACCAAAGACATTTATCTTTATATTAATTCACCGGGTGGTTCTGTTTCAGCTGGT
CTTGCTATTGTTGATACGATGAACTTTATTAAGTCTGATGTTCAAACGATTGTTATGGGTATTGCTGCTTCCATGGGAAC
AATCATCGCTTCTAGCGGTGCCAAAGGAAAACGTTTTATGTTACCAAATGCAGAATATCTTATCCATCAGCCAATGGGCG
GTACAGGCGGTGGCACACAGCAGTCTGATATGGCTATTGCTGCCGAACAATTGCTTAAAACACGCAAAAAATTAGAAAAG
ATTCTATCGGATAATTCTGGAAAAACAATAAAACAAATTCATAAGGATGCTGAACGTGATTATTGGATGGATGCTAAGGA
AACATTAAAGTATGGCTTCATCGATGAAATCATGGAAAATAATGAATTAAAATAA

Domains

Predicted by InterproScan.

• Clp protease (CLP_protease) (19-200)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
clpP	Streptococcus pyogenes MGAS315	90.816	100	0.908
clpP	Streptococcus pyogenes JRS4	90.816	100	0.908
clpP	Streptococcus thermophilus LMG 18311	87.755	100	0.878
clpP	Streptococcus thermophilus LMD-9	87.755	100	0.878
clpP	Lactococcus lactis subsp. lactis strain DGCC12653	87.755	98.492	0.864
clpP	Lactococcus lactis subsp. cremoris KW2	87.245	98.492	0.859
clpP	Streptococcus pneumoniae TIGR4	85.714	100	0.857
clpP	Streptococcus pneumoniae R6	85.714	100	0.857
clpP	Streptococcus pneumoniae D39	85.714	100	0.857
clpP	Streptococcus pneumoniae Rx1	85.714	100	0.857
clpP	Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819	55.959	99.485	0.557
clpP	Bacillus subtilis subsp. subtilis str. 168	54.822	100	0.548

References

• [1]: Satya D Pandey et al. (2022) Clp ATPases differentially affect natural competence development in Streptococcus mutans. MicrobiologyOpen 11(3):e1288. [PMID: 35765180]
• [2]: M Son et al. (2018) Threshold regulation and stochasticity from the MecA/ClpCP proteolytic system in Streptococcus mutans competence. Molecular Microbiology 110(6):914-930. [PMID: 29873131]
• [3]: Gaofeng Dong et al. (2014) Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence. BMC Microbiology 14:183. [PMID: 25005884]